The Resource Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases, Matthew Jenner

Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases, Matthew Jenner

Label
Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases
Title
Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases
Statement of responsibility
Matthew Jenner
Creator
Author
Subject
Genre
Language
eng
Summary
This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully studied the specificity of a range of KS domains from the bacillaene and psymberin PKSs with regard to the initial acylation step of KS-catalysis. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. The documentation of this research is a useful reference and guideline for students starting a PhD in this field
Member of
Cataloging source
N$T
http://library.link/vocab/creatorName
Matthew, Jenner
Dewey number
572/.45
Illustrations
illustrations
Index
no index present
LC call number
QP752.P65
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Series statement
Springer theses
http://library.link/vocab/subjectName
  • Polyketides
  • Mass spectrometry
  • SCIENCE
  • Mass spectrometry
  • Enzymology
  • Biochemical engineering
  • Biochemistry
  • Spectrum analysis, spectrochemistry, mass spectrometry
Label
Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases, Matthew Jenner
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-3-319-32723-5
Instantiates
Publication
Note
"Doctoral thesis accepted by the University of Nottingham, UK."
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Parts of this thesis have been published in the following journal articles:; Supervisor's Foreword; Abstract; Acknowledgements; Contents; Abbreviations; 1 Introduction; 1.1 Natural Products; 1.1.1 Polyketides and Non-ribosomal Peptides; 1.2 Polyketide Synthases; 1.2.1 Polyketide Biosynthesis; 1.2.2 Polyketide Synthase Classification; 1.2.2.1 Type I PKSs; Iterative Type I PKSs; Modular Type I PKSs; The Principle of Colinearity; cis- and trans-AT PKSs; KS Specificity-Based Assignment of trans-AT PKSs; 1.2.2.2 Type II PKSs; 1.2.2.3 Type III PKSs; 1.2.2.4 Non-ribosomal Peptide Synthases
  • 1.2.2.5 Hybrid NRPS-PKS Systems1.2.2.6 Bacillaene: Properties, Structure and Biosynthesis; 1.2.2.7 Psymberin: Properties, Structure and Biosynthesis; 1.2.3 Structure and Mechanisms of PKS Domains; 1.2.3.1 The Ketosynthase Domain; 1.2.3.2 Loading and Non-elongating Ketosynthase Domains; 1.2.3.3 The Acyltransferase Domain; 1.2.3.4 The Acyl Carrier Protein; 1.2.3.5 The Ketoreductase, Dehydratase and Enoylreductase Domains; 1.2.3.6 The Thioesterase Domain; 1.2.4 Architechture of the 'Megasynthase'; 1.2.5 In Vitro Techniques for Studying PKSs; 1.3 Mass Spectrometry; 1.3.1 Ionisation Methods
  • 1.3.1.1 Electrospray Ionisation1.3.1.2 Nano-Electrospray Ionisation; 1.3.1.3 Analysing Proteins by ESI-MS; 1.3.2 Mass Analysers; 1.3.2.1 Quadrupole Analysers; 1.3.2.2 Time-of-Flight Analysers; 1.3.2.3 Hybrid Mass Analyser Instruments; 1.3.2.4 Tandem Mass Spectrometry; Collision Induced Dissociation; References; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments; 2.1.1.1 Protein Production, Purification and Mutagenesis; 2.1.1.2 Mass Spectrometry; Waters SYNAPT HDMS; Nanospray Capillaries; 2.1.2 Buffers and Reagents; 2.1.2.1 Reagents; 2.1.2.2 Transformation and Expression Media
  • Cloning of PsyA ACP1-KS1 Didomain2.2.2 Synthesis of N-Acetylcysteamine Thioesters; 2.2.3 Ketosynthase Acylation Assay; 2.2.4 Synthesis of Acyl-Acyl Carrier Proteins; 2.2.5 Acyl-ACP Ketosynthase Loading Assay; 2.2.6 PedCPedD(R97Q) Hydrolase Assays; 2.2.7 Acyltransferase Extender Unit Specificity Assays; 2.2.8 PedD Malonyl Loading and Unloading of ACP; 2.2.9 Ketosynthase Elongation Assay; 2.2.9.1 Synthesis of Malonyl-ACP; 2.2.9.2 Synthesis of Alkyl-ACP; 2.2.9.3 Ketosynthase Elongation Reactions and MS Analysis; 2.2.10 Monitoring Acyl-Transfer in PsyA ACP1-KS1
Dimensions
unknown
Extent
1 online resource (xviii, 176 pages)
File format
unknown
Form of item
online
Isbn
9783319327235
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
SpringerLink
Other control number
10.1007/978-3-319-32723-5
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)948297002
  • (OCoLC)ocn948297002
Label
Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases, Matthew Jenner
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-3-319-32723-5
Publication
Note
"Doctoral thesis accepted by the University of Nottingham, UK."
Antecedent source
unknown
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Parts of this thesis have been published in the following journal articles:; Supervisor's Foreword; Abstract; Acknowledgements; Contents; Abbreviations; 1 Introduction; 1.1 Natural Products; 1.1.1 Polyketides and Non-ribosomal Peptides; 1.2 Polyketide Synthases; 1.2.1 Polyketide Biosynthesis; 1.2.2 Polyketide Synthase Classification; 1.2.2.1 Type I PKSs; Iterative Type I PKSs; Modular Type I PKSs; The Principle of Colinearity; cis- and trans-AT PKSs; KS Specificity-Based Assignment of trans-AT PKSs; 1.2.2.2 Type II PKSs; 1.2.2.3 Type III PKSs; 1.2.2.4 Non-ribosomal Peptide Synthases
  • 1.2.2.5 Hybrid NRPS-PKS Systems1.2.2.6 Bacillaene: Properties, Structure and Biosynthesis; 1.2.2.7 Psymberin: Properties, Structure and Biosynthesis; 1.2.3 Structure and Mechanisms of PKS Domains; 1.2.3.1 The Ketosynthase Domain; 1.2.3.2 Loading and Non-elongating Ketosynthase Domains; 1.2.3.3 The Acyltransferase Domain; 1.2.3.4 The Acyl Carrier Protein; 1.2.3.5 The Ketoreductase, Dehydratase and Enoylreductase Domains; 1.2.3.6 The Thioesterase Domain; 1.2.4 Architechture of the 'Megasynthase'; 1.2.5 In Vitro Techniques for Studying PKSs; 1.3 Mass Spectrometry; 1.3.1 Ionisation Methods
  • 1.3.1.1 Electrospray Ionisation1.3.1.2 Nano-Electrospray Ionisation; 1.3.1.3 Analysing Proteins by ESI-MS; 1.3.2 Mass Analysers; 1.3.2.1 Quadrupole Analysers; 1.3.2.2 Time-of-Flight Analysers; 1.3.2.3 Hybrid Mass Analyser Instruments; 1.3.2.4 Tandem Mass Spectrometry; Collision Induced Dissociation; References; 2 Materials and Methods; 2.1 Materials; 2.1.1 Instruments; 2.1.1.1 Protein Production, Purification and Mutagenesis; 2.1.1.2 Mass Spectrometry; Waters SYNAPT HDMS; Nanospray Capillaries; 2.1.2 Buffers and Reagents; 2.1.2.1 Reagents; 2.1.2.2 Transformation and Expression Media
  • Cloning of PsyA ACP1-KS1 Didomain2.2.2 Synthesis of N-Acetylcysteamine Thioesters; 2.2.3 Ketosynthase Acylation Assay; 2.2.4 Synthesis of Acyl-Acyl Carrier Proteins; 2.2.5 Acyl-ACP Ketosynthase Loading Assay; 2.2.6 PedCPedD(R97Q) Hydrolase Assays; 2.2.7 Acyltransferase Extender Unit Specificity Assays; 2.2.8 PedD Malonyl Loading and Unloading of ACP; 2.2.9 Ketosynthase Elongation Assay; 2.2.9.1 Synthesis of Malonyl-ACP; 2.2.9.2 Synthesis of Alkyl-ACP; 2.2.9.3 Ketosynthase Elongation Reactions and MS Analysis; 2.2.10 Monitoring Acyl-Transfer in PsyA ACP1-KS1
Dimensions
unknown
Extent
1 online resource (xviii, 176 pages)
File format
unknown
Form of item
online
Isbn
9783319327235
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
SpringerLink
Other control number
10.1007/978-3-319-32723-5
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)948297002
  • (OCoLC)ocn948297002

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