The Resource Transplantation Immunology : Methods and Protocols, edited by Andrea A. Zachary, Mary S. Leffell, (electronic resource)

Transplantation Immunology : Methods and Protocols, edited by Andrea A. Zachary, Mary S. Leffell, (electronic resource)

Label
Transplantation Immunology : Methods and Protocols
Title
Transplantation Immunology
Title remainder
Methods and Protocols
Statement of responsibility
edited by Andrea A. Zachary, Mary S. Leffell
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
After decades of research in clinical transplantation, new techniques have been developed that permit a further understanding of the immune mechanisms underlying immune recognition of allografts and a more accurate and thorough evaluation of compatibility between donors and recipients. The second edition of Transplantation Immunology: Methods and Protocols expands upon the previous edition with current, detailed methods in transplantation immunology. The new methods chapters cover four major areas that are being applied in compatibility evaluations and ongoing transplantation immunology research. Seven overview chapters provide reviews of the molecular basis for alloreactivity, current understanding of humoral and cellular mechanisms, as well as new developments in thoracic organ transplantation, composite tissue transplantation and in the transplantation of sensitized patients. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transplantation Immunology: Methods and Protocol, Second Edition is devoted to transplantation immunology, both in the practice of compatibility testing and in transplantation research
Member of
Dewey number
616.07/9
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • NSURgUuV2qA
  • ciXxMbKJvoc
Image bit depth
0
Language note
English
LC call number
QR180-189.5
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Zachary, Andrea A.
  • Leffell, Mary S.
Series statement
Methods in Molecular Biology,
Series volume
1034
http://library.link/vocab/subjectName
  • Immunology
  • Transplantation of organs, tissu
  • Immunology
  • Transplant Surgery
Label
Transplantation Immunology : Methods and Protocols, edited by Andrea A. Zachary, Mary S. Leffell, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Alloreactivity -- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of Injury -- Cell Mediated Rejection -- Transplantation Tolerance -- Composite Tissue Transplantation -- Transplantation of the Sensitized Patient: Histocompatibility Testing -- Thoracic Organ Transplantation: Laboratory Methods -- HLA Typing by Sequence Specific Primers -- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing -- Next-Generation HLA Sequencing using the 454 GS FLX System -- Chimerism Testing by Quantitative PCR Using Indel Markers -- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR) -- Complement-dependent Cytotoxicity Crossmatch -- Lymphocyte Crossmatching by Flow Cytometry -- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies -- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens -- Tetramer Staining for the Detection of HLA-specific B cells -- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood -- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) -- Cylex ImmuKnow Cell Function Assay -- Detection of Intracellular Cytokines -- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients -- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA -- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing
Dimensions
unknown
Edition
2nd ed. 2013.
Extent
1 online resource (XIII, 411 p. 49 illus., 29 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627034937
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-62703-493-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3280000000033920
  • (SSID)ssj0000984053
  • (PQKBManifestationID)12401064
  • (PQKBTitleCode)TC0000984053
  • (PQKBWorkID)11011585
  • (PQKB)11523651
  • (DE-He213)978-1-62703-493-7
  • (EXLCZ)993280000000033920
Label
Transplantation Immunology : Methods and Protocols, edited by Andrea A. Zachary, Mary S. Leffell, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Alloreactivity -- Antibodies in Transplantation: The Effects of HLA and Non-HLA Antibody Binding and Mechanisms of Injury -- Cell Mediated Rejection -- Transplantation Tolerance -- Composite Tissue Transplantation -- Transplantation of the Sensitized Patient: Histocompatibility Testing -- Thoracic Organ Transplantation: Laboratory Methods -- HLA Typing by Sequence Specific Primers -- Human Leukocyte Antigen (HLA) Typing By DNA Sequencing -- Next-Generation HLA Sequencing using the 454 GS FLX System -- Chimerism Testing by Quantitative PCR Using Indel Markers -- Gene-specific PCR Typing of Killer Cell Immunoglobulin-like Receptors (KIR) -- Complement-dependent Cytotoxicity Crossmatch -- Lymphocyte Crossmatching by Flow Cytometry -- Solid Phase Assay Measuring C4d Deposition to Determine Complement Fixation by HLA Specific Antibodies -- C1q Assay for the Detection of Complement Fixing Antibody to HLA Antigens -- Tetramer Staining for the Detection of HLA-specific B cells -- A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood -- Detection of Antibodies to Self-antigens (K-alpha 1 Tubulin, Collagen I, II, IV and V Myosin and Vimentin) by Enzyme-Linked Immunosorbent Assay (ELISA) -- Cylex ImmuKnow Cell Function Assay -- Detection of Intracellular Cytokines -- Tolerogenic Dendritic Cells and Induction of T Suppresson Cells in Transplant Recipients -- Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA -- RNA Purification and Expression Analysis using Microarrays and RNA Deep Sequencing
Dimensions
unknown
Edition
2nd ed. 2013.
Extent
1 online resource (XIII, 411 p. 49 illus., 29 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627034937
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-62703-493-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3280000000033920
  • (SSID)ssj0000984053
  • (PQKBManifestationID)12401064
  • (PQKBTitleCode)TC0000984053
  • (PQKBWorkID)11011585
  • (PQKB)11523651
  • (DE-He213)978-1-62703-493-7
  • (EXLCZ)993280000000033920

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