The Resource Transgenesis techniques : principles and protocols, (electronic resource)

Transgenesis techniques : principles and protocols, (electronic resource)

Label
Transgenesis techniques : principles and protocols
Title
Transgenesis techniques
Title remainder
principles and protocols
Contributor
Subject
Genre
Language
eng
Summary
One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques
Member of
Cataloging source
CUS
Dewey number
660.6/5
Illustrations
illustrations
Index
index present
LC call number
QH442.6
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Cartwright, Elizabeth J
Series statement
  • Springer protocols
  • Methods in molecular biology
Series volume
561
http://library.link/vocab/subjectName
  • Transgenic animals
  • Animal genetic engineering
  • Animals, Genetically Modified
  • Genetic Engineering
  • Genetic Engineering
  • Transformation, Genetic
  • Animal genetic engineering
  • Transgenic animals
Label
Transgenesis techniques : principles and protocols, (electronic resource)
Link
http://dx.doi.org/10.1007/978-1-60327-019-9
Instantiates
Publication
Note
Earlier edition published 2002
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Transgenesis in Drosophila melanogaster / L. Ringrose -- Transgenesis in Caenorhabditis elegans / M. Rieckher [and others] -- Transgenesis in zebrafish with the tol2 transposon system / M.L. Suster [and others] -- Generation of transgenic frogs / J. Loeber, F.C. Pan and T. Pieler -- Pronuclear DNA injection for the production of transgenic rats / J. Cozzi [and others] -- Cell-type-specific transgenesis in the mouse / J. Gulick and J. Robbins -- Transgene design and delivery into the mouse genome: keys to success / L. Teboul -- Overexpression transgenesis in mouse: pronuclear injection / W.J. Gardiner and L. Teboul -- Gene-targeting vectors / J. Simon, C. Arthur and V.A. McGuire -- Gene trap: knockout on the fast lane / M. Ullrich and K. Schuh -- Culture of murine embryonic stem cells / I. Barbaric and T.N. Dear -- Targeting embryonic stem cells / R.H. Friedel -- Generation of chimeras by microinjection / A. Pluck and C. Klasen -- Generation of chimeras by morula aggregation / A. Pluck and C. Klasen -- Surgical techniques for the generation of mutant mice / A. Pluck and C. Klasen -- Site-specific recombinases for manipulation of the mouse genome / M.C. Birling, F. Gofflot and X. Warot -- Cre transgenic mouse lines / X. Wang -- Large-scale mouse mutagenesis / E.J. Cartwright -- Dedicated mouse production and husbandry / L. Vizor and S. Wells -- Biological methods for archiving and maintaining mutant laboratory mice. Part I: conserving mutant strains / M.D. Fray -- Biological methods for archiving and maintaining mutant laboratory mice. Part II: recovery and distribution of conserved mutant strains / M.D. Fray
Dimensions
unknown
Edition
  • 3rd ed. /
  • edited by Elizabeth J. Cartwright.
Extent
1 online resource (xii, 335 pages)
Form of item
online
Isbn
9781603270199
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)434488215
  • (OCoLC)ocn434488215
Label
Transgenesis techniques : principles and protocols, (electronic resource)
Link
http://dx.doi.org/10.1007/978-1-60327-019-9
Publication
Note
Earlier edition published 2002
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Transgenesis in Drosophila melanogaster / L. Ringrose -- Transgenesis in Caenorhabditis elegans / M. Rieckher [and others] -- Transgenesis in zebrafish with the tol2 transposon system / M.L. Suster [and others] -- Generation of transgenic frogs / J. Loeber, F.C. Pan and T. Pieler -- Pronuclear DNA injection for the production of transgenic rats / J. Cozzi [and others] -- Cell-type-specific transgenesis in the mouse / J. Gulick and J. Robbins -- Transgene design and delivery into the mouse genome: keys to success / L. Teboul -- Overexpression transgenesis in mouse: pronuclear injection / W.J. Gardiner and L. Teboul -- Gene-targeting vectors / J. Simon, C. Arthur and V.A. McGuire -- Gene trap: knockout on the fast lane / M. Ullrich and K. Schuh -- Culture of murine embryonic stem cells / I. Barbaric and T.N. Dear -- Targeting embryonic stem cells / R.H. Friedel -- Generation of chimeras by microinjection / A. Pluck and C. Klasen -- Generation of chimeras by morula aggregation / A. Pluck and C. Klasen -- Surgical techniques for the generation of mutant mice / A. Pluck and C. Klasen -- Site-specific recombinases for manipulation of the mouse genome / M.C. Birling, F. Gofflot and X. Warot -- Cre transgenic mouse lines / X. Wang -- Large-scale mouse mutagenesis / E.J. Cartwright -- Dedicated mouse production and husbandry / L. Vizor and S. Wells -- Biological methods for archiving and maintaining mutant laboratory mice. Part I: conserving mutant strains / M.D. Fray -- Biological methods for archiving and maintaining mutant laboratory mice. Part II: recovery and distribution of conserved mutant strains / M.D. Fray
Dimensions
unknown
Edition
  • 3rd ed. /
  • edited by Elizabeth J. Cartwright.
Extent
1 online resource (xii, 335 pages)
Form of item
online
Isbn
9781603270199
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)434488215
  • (OCoLC)ocn434488215

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