The Resource Transgenesis techniques : principles and protocols, edited by David Murphy, David A. Carter, (electronic resource)

Transgenesis techniques : principles and protocols, edited by David Murphy, David A. Carter, (electronic resource)

Label
Transgenesis techniques : principles and protocols
Title
Transgenesis techniques
Title remainder
principles and protocols
Statement of responsibility
edited by David Murphy, David A. Carter
Contributor
Subject
Genre
Language
eng
Summary
Practical methods for the generation and analysis of transgenic animals, covering a comprehensive range of species, from invertebrates to lower vertebrates to higher vertebrates. Chapters examine ways in which transgenic techniques can be used to address many problems in biological systems present in-depth descriptions of analytical methods, and give step-by-step techniques that can be easily performed in your lab
Member of
Additional physical form
Also available in print.
Cataloging source
DAY
Dewey number
591.1/5/0724
Illustrations
illustrations
Index
index present
LC call number
QH442.6
LC item number
.T66 1993
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 442.6
NLM item number
  • ME9616J v.18 1993
  • T772 1993
http://library.link/vocab/relatedWorkOrContributorDate
  • 1957-
  • 1954-
http://library.link/vocab/relatedWorkOrContributorName
  • Murphy, David
  • Carter, David A.
Series statement
Methods in molecular biology
Series volume
v. 18
http://library.link/vocab/subjectName
  • Animal genetic engineering
  • Transgenic animals
  • Genetic transformation
  • Genetic Engineering
  • Transformation, Genetic
  • Animal genetic engineering
  • Genetic transformation
  • Transgenic animals
  • Animal genetic engineering -- Laboratory manuals
  • Electronic books. -- local
  • Transgenic animals -- Laboratory manuals
Target audience
adult
Label
Transgenesis techniques : principles and protocols, edited by David Murphy, David A. Carter, (electronic resource)
Link
http://dx.doi.org/10.1385/0896032450
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Introduction to transgenesis -- Transgenic rodents and the study of the central nervous system -- Transgenic animals and the study of cancer -- Transgenic animals and the study of the immune system -- Commercial and agricultural applications of animal transgenesis -- P element-mediated germ-line transformation of Drosophila -- Gene transfer by microinjection in the zebra fish Brachydanio rerio -- Transgenic induction in salmonid and tilapia fish -- Overview of transgenic mouse production -- Transgene design -- Isolation of DNA fragments for microinjection -- Establishing a colony for efficient production of transgenic mice -- Mating mice -- Anesthetizing mice -- Vasectomizing a mouse -- Preparation of culture media for fertilized one-cell mouse eggs -- Collection of fertilized one-cell mouse eggs for microinjection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs. 1, Manual injection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs. 2, Automatic injection -- Delivery of microinjected eggs to surrogate mothers by oviduct transfer -- Caesarean section and fostering -- In vitro fertilization of mouse eggs -- In vitro isolation of murine embryonic stem cells -- Production of chimeras derived from murine embryonic stem cells -- Cryopreservation of transgenic mouse lines -- Introduction to the physiology and husbandry of the rat -- Mating of rats -- Vasectomy of the male rat -- Harvest of fertilized one-cell rat eggs -- Implantation of microinjected eggs -- Cryopreservation of transgenic rat lines -- Production of transgenic sheep -- Cryopreservation of transgenic sheep lines -- Isolation of genomic DNA from tail tissue -- Slot blotting of genomic tail DNA -- Genomic analysis of transgenic animals by the polymerase chain reaction -- Genomic analysis of transgenic animals : Southern blotting -- The preparation of RNA from the tissues of transgenic animals -- Northern blotting -- RNA slot blotting -- Analysis of gene expression by PCR -- Nuclear run-on analysis of transcription -- S1 nuclease protection mapping -- Primer extension -- In situ hybridization analysis of transgenic tissues -- Immunohistochemical analysis of transgene expression -- Postmortem examination of transgenic mice -- Histological examination of transgenic mice -- Reporter enzyme assays -- Restriction endonuclease digestion of DNA -- Agarose gel electrophoresis -- Working with radioactivity in the transgenic animal laboratory -- Random primed labeling of DNA -- Filter hybridization -- DNA transfection
Dimensions
unknown
Extent
1 online resource (xii, 467 pages)
File format
multiple file formats
Form of item
online
Governing access note
Access restricted by licensing agreement
Isbn
9781592595051
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
  • (OCoLC)272405334
  • (OCoLC)ocn272405334
System details
  • System requirements: World Wide Web browser, internet connectivity, Adobe Acrobat reader
  • Mode of access: World Wide Web
Label
Transgenesis techniques : principles and protocols, edited by David Murphy, David A. Carter, (electronic resource)
Link
http://dx.doi.org/10.1385/0896032450
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
black and white
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Introduction to transgenesis -- Transgenic rodents and the study of the central nervous system -- Transgenic animals and the study of cancer -- Transgenic animals and the study of the immune system -- Commercial and agricultural applications of animal transgenesis -- P element-mediated germ-line transformation of Drosophila -- Gene transfer by microinjection in the zebra fish Brachydanio rerio -- Transgenic induction in salmonid and tilapia fish -- Overview of transgenic mouse production -- Transgene design -- Isolation of DNA fragments for microinjection -- Establishing a colony for efficient production of transgenic mice -- Mating mice -- Anesthetizing mice -- Vasectomizing a mouse -- Preparation of culture media for fertilized one-cell mouse eggs -- Collection of fertilized one-cell mouse eggs for microinjection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs. 1, Manual injection -- Microinjection of cloned DNA fragments into fertilized one-cell mouse eggs. 2, Automatic injection -- Delivery of microinjected eggs to surrogate mothers by oviduct transfer -- Caesarean section and fostering -- In vitro fertilization of mouse eggs -- In vitro isolation of murine embryonic stem cells -- Production of chimeras derived from murine embryonic stem cells -- Cryopreservation of transgenic mouse lines -- Introduction to the physiology and husbandry of the rat -- Mating of rats -- Vasectomy of the male rat -- Harvest of fertilized one-cell rat eggs -- Implantation of microinjected eggs -- Cryopreservation of transgenic rat lines -- Production of transgenic sheep -- Cryopreservation of transgenic sheep lines -- Isolation of genomic DNA from tail tissue -- Slot blotting of genomic tail DNA -- Genomic analysis of transgenic animals by the polymerase chain reaction -- Genomic analysis of transgenic animals : Southern blotting -- The preparation of RNA from the tissues of transgenic animals -- Northern blotting -- RNA slot blotting -- Analysis of gene expression by PCR -- Nuclear run-on analysis of transcription -- S1 nuclease protection mapping -- Primer extension -- In situ hybridization analysis of transgenic tissues -- Immunohistochemical analysis of transgene expression -- Postmortem examination of transgenic mice -- Histological examination of transgenic mice -- Reporter enzyme assays -- Restriction endonuclease digestion of DNA -- Agarose gel electrophoresis -- Working with radioactivity in the transgenic animal laboratory -- Random primed labeling of DNA -- Filter hybridization -- DNA transfection
Dimensions
unknown
Extent
1 online resource (xii, 467 pages)
File format
multiple file formats
Form of item
online
Governing access note
Access restricted by licensing agreement
Isbn
9781592595051
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
  • (OCoLC)272405334
  • (OCoLC)ocn272405334
System details
  • System requirements: World Wide Web browser, internet connectivity, Adobe Acrobat reader
  • Mode of access: World Wide Web

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