The Resource The Nuclear Envelope : Methods and Protocols, edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer, (electronic resource)

The Nuclear Envelope : Methods and Protocols, edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer, (electronic resource)

Label
The Nuclear Envelope : Methods and Protocols
Title
The Nuclear Envelope
Title remainder
Methods and Protocols
Statement of responsibility
edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer
Contributor
Editor
Editor
Subject
Language
eng
Summary
This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nuclear Envelope: Methods and Protocols is divided into five sections: Part I – Nuclear Envelope Isolation; Part II – Nuclear Envelope Protein Interactions, Localization, and Dynamics; Part III – Nuclear Envelope Interactions with the Cytoskeleton; Part IV – Nuclear Envelope-Chromatin Interactions; and Part V – Nucleo-Cytoplasmic Transport. Many of the modifications discussed in this book have only been circulated within laboratories that have conducted research in this field for many years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field
Member of
Dewey number
571.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • kdkSfoIwbwI
  • iXPUllybdq4
  • 8L_YbnqfFdM
Image bit depth
0
LC call number
QH573-671
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Shackleton, Sue.
  • Collas, Philippe.
  • Schirmer, Eric C.
Series statement
Methods in Molecular Biology,
Series volume
1411
http://library.link/vocab/subjectName
  • Cytology
  • Cell Biology
Label
The Nuclear Envelope : Methods and Protocols, edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer, (electronic resource)
Instantiates
Publication
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome -- Exploring the Protein Composition of the Plant Nuclear Envelope -- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes -- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins -- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) -- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP -- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro -- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID -- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners -- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components -- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation -- Analysis of Meiotic Telomere Behavior in the Mouse -- Identification and Validation of Putative Nesprin Variants -- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting -- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties -- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling -- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts -- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells -- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila -- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins -- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion -- DamID Analysis of Nuclear Organization in Caenorhabditis elegans -- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells -- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization -- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System -- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina -- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex -- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane -- Nuclear Protein Transport in Digitonin Permeabilized Cells -- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells -- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport
Dimensions
unknown
Edition
1st ed. 2016.
Extent
1 online resource (XIV, 523 p. 103 illus., 77 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781493935307
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-3530-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000717951
  • (DE-He213)978-1-4939-3530-7
  • (EXLCZ)993710000000717951
Label
The Nuclear Envelope : Methods and Protocols, edited by Sue Shackleton, Philippe Collas, Eric C. Schirmer, (electronic resource)
Publication
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
not applicable
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome -- Exploring the Protein Composition of the Plant Nuclear Envelope -- High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes -- Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins -- Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) -- Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP -- The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro -- Identifying Protein-Protein Associations at the Nuclear Envelope with BioID -- In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners -- Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components -- Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation -- Analysis of Meiotic Telomere Behavior in the Mouse -- Identification and Validation of Putative Nesprin Variants -- Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting -- The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties -- Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling -- Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts -- Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells -- Imaging Approaches to Investigate Myonuclear Positioning in Drosophila -- Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins -- Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion -- DamID Analysis of Nuclear Organization in Caenorhabditis elegans -- The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells -- Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization -- Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System -- Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina -- High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex -- An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane -- Nuclear Protein Transport in Digitonin Permeabilized Cells -- Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells -- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport
Dimensions
unknown
Edition
1st ed. 2016.
Extent
1 online resource (XIV, 523 p. 103 illus., 77 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781493935307
Level of compression
uncompressed
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-3530-7
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000717951
  • (DE-He213)978-1-4939-3530-7
  • (EXLCZ)993710000000717951

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