The Resource T Cell Protocols : Development and Activation, edited by Kelly P. Kearse, (electronic resource)

T Cell Protocols : Development and Activation, edited by Kelly P. Kearse, (electronic resource)

Label
T Cell Protocols : Development and Activation
Title
T Cell Protocols
Title remainder
Development and Activation
Statement of responsibility
edited by Kelly P. Kearse
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
In T Cell Protocols: Development and Activation, Kelly P. Kearse and a panel of laboratory experts describe the step-by-step execution of a wide variety of basic and specialized techniques for the study of T cell developmental biology and of their subsequent activation. Readily reproducible, the techniques are explained concisely, but in such detail that they are suitable for both the novice and experienced investigator. Coverage includes treatment of both fundamental and novel experimental protocols for the analysis of the role T cells play in immune function and in the prevention and cure of human diseases. Practical and timely, T Cell Protocols: Development and Activation offers basic scientists and clinical investigators a valuable compendium of key protocols that will improve skills with existing methods, as well as afford rapid mastery of powerful new techniques
Member of
Is Subseries of
Dewey number
616.07/97
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
HsVLpSdJ__o
Language note
English
LC call number
QR180-189.5
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Kearse, Kelly P.
Series statement
Methods in Molecular Biology,
Series volume
134
http://library.link/vocab/subjectName
  • Immunology
  • Immunology
Label
T Cell Protocols : Development and Activation, edited by Kelly P. Kearse, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Abbreviated title
  • T CELL PROTOCOLS
  • T CELL PROTOCOLS: DEVELOPMENT & ACTIVATION
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Overview: For Chapters 2 – 15 -- Insights into T-Cell Development Using Transgenic and Knockout Mice -- T Cell Development -- Isolation and Characterization of Murine Early Intrathymic Precursor Populations -- Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture -- Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody -- Transfection and Transcription of Genes in Developing Thymocytes -- Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay -- Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses -- Quantitative Analysis of the Usage of Human T-Cell Receptor ? and ? Chain Variable Regions by Reverse Dot Blot Hybridization -- Flow Cytometric Detection of Cytoplasmic and Surface CD3-? Expression in Developing T Cells -- Assessing Apoptosis of Developing T Cells by Flow Cytometry -- Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS) -- Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning -- Flow Cytometric Analysis of Murine T Lymphocytes -- Production and Characterization of Immature Murine T-Lymphoma Cell Lines -- Production and Characterization of T-Cell Hybridomas -- Overview for Chapters 17–29 -- Something Happens; A Brief History of TCR Signal Transduction -- T-Cell Activation -- Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes -- Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation -- Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry -- Digital Image Analysis of Lymphocyte Activation -- Induction of T-Cell Signaling by Immobilized Integrin Ligands -- Cytotoxic T-Cell Adherence Assay (CAA) -- Detecting Ubiquitinated T-Cell Antigen Receptor Subunits by Immunoblotting -- Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes -- Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry -- Biochemical Analysis of Activated T Lymphocytes -- Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement -- Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4+ T Cells -- Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA)
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (381 p.)
Form of item
online
Isbn
9781280831577
Media category
computer
Media type code
  • c
Other control number
10.1385/1592596827
Specific material designation
remote
System control number
  • (CKB)1000000000240165
  • (EBL)3037165
  • (SSID)ssj0000255022
  • (PQKBManifestationID)11213146
  • (PQKBTitleCode)TC0000255022
  • (PQKBWorkID)10211179
  • (PQKB)10440277
  • (MiAaPQ)EBC3037165
  • (DE-He213)978-1-59259-682-9
  • (EXLCZ)991000000000240165
Label
T Cell Protocols : Development and Activation, edited by Kelly P. Kearse, (electronic resource)
Publication
Note
Description based upon print version of record
Abbreviated title
  • T CELL PROTOCOLS
  • T CELL PROTOCOLS: DEVELOPMENT & ACTIVATION
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Overview: For Chapters 2 – 15 -- Insights into T-Cell Development Using Transgenic and Knockout Mice -- T Cell Development -- Isolation and Characterization of Murine Early Intrathymic Precursor Populations -- Differentiation of Mouse Thymocytes in Fetal Thymus Organ Culture -- Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody -- Transfection and Transcription of Genes in Developing Thymocytes -- Analysis of CD4/CD8 Lineage Commitment by Pronase Treatment and Reexpression Assay -- Intrathymic Delivery of MHC Genes Using Recombinant Adenoviruses -- Quantitative Analysis of the Usage of Human T-Cell Receptor ? and ? Chain Variable Regions by Reverse Dot Blot Hybridization -- Flow Cytometric Detection of Cytoplasmic and Surface CD3-? Expression in Developing T Cells -- Assessing Apoptosis of Developing T Cells by Flow Cytometry -- Isolation of T-Cell Subsets by Magnetic Cell Sorting (MACS) -- Isolation of T-Cell Antigens by Retrovirus-Mediated Expression Cloning -- Flow Cytometric Analysis of Murine T Lymphocytes -- Production and Characterization of Immature Murine T-Lymphoma Cell Lines -- Production and Characterization of T-Cell Hybridomas -- Overview for Chapters 17–29 -- Something Happens; A Brief History of TCR Signal Transduction -- T-Cell Activation -- Measurement of Phosphatidylinositol (PI) Hydrolysis in Activated T Lymphocytes -- Fluorescence Polarization as an Early Measure of T-Lymphocyte Stimulation -- Measurement of Lymphoproliferation at the Single-Cell Level by Flow Cytometry -- Digital Image Analysis of Lymphocyte Activation -- Induction of T-Cell Signaling by Immobilized Integrin Ligands -- Cytotoxic T-Cell Adherence Assay (CAA) -- Detecting Ubiquitinated T-Cell Antigen Receptor Subunits by Immunoblotting -- Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes -- Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry -- Biochemical Analysis of Activated T Lymphocytes -- Activation of Heterotrimeric GTP-Binding Proteins Upon TCR/CD3 Engagement -- Generation of Human T Helper 1 and T Helper 2 Subsets from Peripheral Blood-Derived Naive CD4+ T Cells -- Transcriptional Expression Analysis of T-Cell Activation by Multiplex Messenger Assay (MMA)
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (381 p.)
Form of item
online
Isbn
9781280831577
Media category
computer
Media type code
  • c
Other control number
10.1385/1592596827
Specific material designation
remote
System control number
  • (CKB)1000000000240165
  • (EBL)3037165
  • (SSID)ssj0000255022
  • (PQKBManifestationID)11213146
  • (PQKBTitleCode)TC0000255022
  • (PQKBWorkID)10211179
  • (PQKB)10440277
  • (MiAaPQ)EBC3037165
  • (DE-He213)978-1-59259-682-9
  • (EXLCZ)991000000000240165

Library Locations

  • Architecture LibraryBorrow it
    Gould Hall 830 Van Vleet Oval Rm. 105, Norman, OK, 73019, US
    35.205706 -97.445050
  • Bizzell Memorial LibraryBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Boorstin CollectionBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Chinese Literature Translation ArchiveBorrow it
    401 W. Brooks St., RM 414, Norman, OK, 73019, US
    35.207487 -97.447906
  • Engineering LibraryBorrow it
    Felgar Hall 865 Asp Avenue, Rm. 222, Norman, OK, 73019, US
    35.205706 -97.445050
  • Fine Arts LibraryBorrow it
    Catlett Music Center 500 West Boyd Street, Rm. 20, Norman, OK, 73019, US
    35.210371 -97.448244
  • Harry W. Bass Business History CollectionBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • History of Science CollectionsBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • John and Mary Nichols Rare Books and Special CollectionsBorrow it
    401 W. Brooks St., Rm. 509NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • Library Service CenterBorrow it
    2601 Technology Place, Norman, OK, 73019, US
    35.185561 -97.398361
  • Price College Digital LibraryBorrow it
    Adams Hall 102 307 West Brooks St., Norman, OK, 73019, US
    35.210371 -97.448244
  • Western History CollectionsBorrow it
    Monnet Hall 630 Parrington Oval, Rm. 300, Norman, OK, 73019, US
    35.209584 -97.445414
Processing Feedback ...