The Resource Small Non-Coding RNAs : Methods and Protocols, edited by Mathieu Rederstorff, (electronic resource)

Small Non-Coding RNAs : Methods and Protocols, edited by Mathieu Rederstorff, (electronic resource)

Label
Small Non-Coding RNAs : Methods and Protocols
Title
Small Non-Coding RNAs
Title remainder
Methods and Protocols
Statement of responsibility
edited by Mathieu Rederstorff
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular, and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs
Member of
Dewey number
572.8/8
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
f-ZK9F2V8OM
Image bit depth
0
Language note
English
LC call number
  • RB155-155.8
  • QH431
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Rederstorff, Mathieu.
Series statement
Methods in Molecular Biology,
Series volume
1296
http://library.link/vocab/subjectName
  • Human genetics
  • Human Genetics
Label
Small Non-Coding RNAs : Methods and Protocols, edited by Mathieu Rederstorff, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror -- Alcoholic Precipitation of Small Non-Coding RNAs -- Quantification and Quality Control of a Small Non-Coding RNA Preparation -- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting -- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes -- Direct Cloning of Double Stranded RNAs -- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation -- Fluorescence in situ Hybridization of Small Non-Coding RNAs -- RT-qPCR Based Quantification of Small Non-Coding RNAs -- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs -- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs -- Probing Small Non-Coding RNAs Structures -- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing -- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells -- Microarray Analysis of Small Non-Coding RNAs -- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes -- Dual Luciferase Gene Reporter Assays to Study miRNA Function -- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells -- Efficient and Selective Knockdown of Small Non-Coding RNAs -- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands -- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics
Dimensions
unknown
Edition
1st ed. 2015.
Extent
1 online resource (XI, 238 p. 35 illus., 15 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781493925476
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-4939-2547-6
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000379552
  • (SSID)ssj0001546248
  • (PQKBManifestationID)16136416
  • (PQKBTitleCode)TC0001546248
  • (PQKBWorkID)14792561
  • (PQKB)10288695
  • (DE-He213)978-1-4939-2547-6
  • (EXLCZ)993710000000379552
Label
Small Non-Coding RNAs : Methods and Protocols, edited by Mathieu Rederstorff, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror -- Alcoholic Precipitation of Small Non-Coding RNAs -- Quantification and Quality Control of a Small Non-Coding RNA Preparation -- Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting -- Improved Northern Blot Detection of Small RNAs Using EDC Crosslinking and DNA/LNA Probes -- Direct Cloning of Double Stranded RNAs -- Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation -- Fluorescence in situ Hybridization of Small Non-Coding RNAs -- RT-qPCR Based Quantification of Small Non-Coding RNAs -- Stem-Loop RT-PCR Based Quantification of Small Non-Coding RNAs -- miR-RACE: An Effective Approach to Accurately Determine the Sequence of Computationally Identified miRNAs -- Probing Small Non-Coding RNAs Structures -- cDNA Library Generation for the Analysis of Small RNAs by High Throughput Sequencing -- CLIP-seq to Discover Transcriptome-Wide Imprinting of RNA Binding Proteins in Living Cells -- Microarray Analysis of Small Non-Coding RNAs -- RLM-RACE, PPM-RACE and qRT-PCR, an Integrated Strategy to Accurately Validate miRNA Target Genes -- Dual Luciferase Gene Reporter Assays to Study miRNA Function -- Gene Expression Knockdown by Transfection of siRNAs into Mammalian Cells -- Efficient and Selective Knockdown of Small Non-Coding RNAs -- Cell-SELEX: In Vitro Selection of Synthetic Small Specific Ligands -- Small Non-Coding RNAs and Aptamers in Diagnostics and Therapeutics
Dimensions
unknown
Edition
1st ed. 2015.
Extent
1 online resource (XI, 238 p. 35 illus., 15 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781493925476
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-4939-2547-6
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3710000000379552
  • (SSID)ssj0001546248
  • (PQKBManifestationID)16136416
  • (PQKBTitleCode)TC0001546248
  • (PQKBWorkID)14792561
  • (PQKB)10288695
  • (DE-He213)978-1-4939-2547-6
  • (EXLCZ)993710000000379552

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