The Resource Skeletal Development and Repair : Methods and Protocols, edited by Matthew J. Hilton, (electronic resource)

Skeletal Development and Repair : Methods and Protocols, edited by Matthew J. Hilton, (electronic resource)

Label
Skeletal Development and Repair : Methods and Protocols
Title
Skeletal Development and Repair
Title remainder
Methods and Protocols
Statement of responsibility
edited by Matthew J. Hilton
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation, as well as, the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation, and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections, and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research
Member of
Dewey number
611.01816
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
zWw22AVr1iI
Image bit depth
0
Language note
English
LC call number
QH506
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Hilton, Matthew J.
Series statement
Methods in Molecular Biology,
Series volume
1130
http://library.link/vocab/subjectName
  • Medicine
  • Molecular Medicine
  • Regenerative Medicine/Tissue Engineering
Label
Skeletal Development and Repair : Methods and Protocols, edited by Matthew J. Hilton, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Generation of Closed Transverse Fractures in Small Animals -- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool -- Surgical Induction of Posttraumatic Osteoarthirits -- A Mouse Model of Flexor Tendon Repair -- Cartilage Explant Cultures -- Renal Capsule Transplantations to Assay Skeletal Angiogenesis -- Whole Mount Skeletal Staining -- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012) -- Demineralized Murine Skeletal Histology -- Beta-Galactosidase Staining in the Skeleton -- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues -- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections -- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections -- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections -- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis -- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme -- Isolation and Culture of Murine Primary Chondrocytes -- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays
Dimensions
unknown
Edition
1st ed. 2014.
Extent
1 online resource (X, 328 p. 78 illus., 40 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627039895
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-62703-989-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3390000000054038
  • (SSID)ssj0001260896
  • (PQKBManifestationID)12460644
  • (PQKBTitleCode)TC0001260896
  • (PQKBWorkID)11310934
  • (PQKB)10171537
  • (DE-He213)978-1-62703-989-5
  • (EXLCZ)993390000000054038
Label
Skeletal Development and Repair : Methods and Protocols, edited by Matthew J. Hilton, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Overview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Generation of Closed Transverse Fractures in Small Animals -- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool -- Surgical Induction of Posttraumatic Osteoarthirits -- A Mouse Model of Flexor Tendon Repair -- Cartilage Explant Cultures -- Renal Capsule Transplantations to Assay Skeletal Angiogenesis -- Whole Mount Skeletal Staining -- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012) -- Demineralized Murine Skeletal Histology -- Beta-Galactosidase Staining in the Skeleton -- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues -- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections -- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections -- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections -- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis -- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme -- Isolation and Culture of Murine Primary Chondrocytes -- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays
Dimensions
unknown
Edition
1st ed. 2014.
Extent
1 online resource (X, 328 p. 78 illus., 40 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781627039895
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-62703-989-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3390000000054038
  • (SSID)ssj0001260896
  • (PQKBManifestationID)12460644
  • (PQKBTitleCode)TC0001260896
  • (PQKBWorkID)11310934
  • (PQKB)10171537
  • (DE-He213)978-1-62703-989-5
  • (EXLCZ)993390000000054038

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