The Resource SV40 protocols, edited by Leda Raptis, (electronic resource)

SV40 protocols, edited by Leda Raptis, (electronic resource)

Label
SV40 protocols
Title
SV40 protocols
Statement of responsibility
edited by Leda Raptis
Title variation
Simian virus 40 protocols
Contributor
Subject
Genre
Language
eng
Summary
Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources. Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors
Member of
Cataloging source
COO
Dewey number
579.2/445
Illustrations
illustrations
Index
index present
LC call number
QR406.2.S56
LC item number
S87 2001
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 165
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QW 165.5.P2
NLM item number
  • ME9616J v.165 2001
  • S969 2001
http://library.link/vocab/relatedWorkOrContributorName
Raptis, Leda
Series statement
Methods in molecular biology
Series volume
v. 165
http://library.link/vocab/subjectName
  • SV40 (Virus)
  • Simian virus 40
  • Genetic Techniques
  • SCIENCE
  • SCIENCE
  • SV40 (Virus)
Label
SV40 protocols, edited by Leda Raptis, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591175
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen
Dimensions
unknown
Extent
1 online resource (xi, 310 pages)
Form of item
online
Isbn
9780896036536
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)55684334
  • (OCoLC)ocm55684334
Label
SV40 protocols, edited by Leda Raptis, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591175
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen
Dimensions
unknown
Extent
1 online resource (xi, 310 pages)
Form of item
online
Isbn
9780896036536
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)55684334
  • (OCoLC)ocm55684334

Library Locations

  • Architecture LibraryBorrow it
    Gould Hall 830 Van Vleet Oval Rm. 105, Norman, OK, 73019, US
    35.205706 -97.445050
  • Bizzell Memorial LibraryBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Boorstin CollectionBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Chinese Literature Translation ArchiveBorrow it
    401 W. Brooks St., RM 414, Norman, OK, 73019, US
    35.207487 -97.447906
  • Engineering LibraryBorrow it
    Felgar Hall 865 Asp Avenue, Rm. 222, Norman, OK, 73019, US
    35.205706 -97.445050
  • Fine Arts LibraryBorrow it
    Catlett Music Center 500 West Boyd Street, Rm. 20, Norman, OK, 73019, US
    35.210371 -97.448244
  • Harry W. Bass Business History CollectionBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • History of Science CollectionsBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • John and Mary Nichols Rare Books and Special CollectionsBorrow it
    401 W. Brooks St., Rm. 509NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • Library Service CenterBorrow it
    2601 Technology Place, Norman, OK, 73019, US
    35.185561 -97.398361
  • Price College Digital LibraryBorrow it
    Adams Hall 102 307 West Brooks St., Norman, OK, 73019, US
    35.210371 -97.448244
  • Western History CollectionsBorrow it
    Monnet Hall 630 Parrington Oval, Rm. 300, Norman, OK, 73019, US
    35.209584 -97.445414
Processing Feedback ...