The Resource Receptor Signal Transduction Protocols : Third Edition, edited by Gary B. Willars, R.A. John Challiss, (electronic resource)

Receptor Signal Transduction Protocols : Third Edition, edited by Gary B. Willars, R.A. John Challiss, (electronic resource)

Label
Receptor Signal Transduction Protocols : Third Edition
Title
Receptor Signal Transduction Protocols
Title remainder
Third Edition
Statement of responsibility
edited by Gary B. Willars, R.A. John Challiss
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues. Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field
Member of
Is Subseries of
Dewey number
572/.696
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • S8m9VgcBRFw
  • Z4uAqLzPbso
Image bit depth
0
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Willars, Gary B.
  • Challiss, R.A. John.
Series statement
Methods in Molecular Biology,
Series volume
746
http://library.link/vocab/subjectName
  • Biochemistry
  • Biochemistry, general
Label
Receptor Signal Transduction Protocols : Third Edition, edited by Gary B. Willars, R.A. John Challiss, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Inducible Expression of G Protein-Coupled Receptors in Transfected Cells -- Using the Flp-InTM T-RexTM System to Regulate GPCR Expression -- Viral Infection for GPCR Expression in Eukaryotic Cells -- Generation of Epitope-Tagged GPCRs -- The Use of Site-Directed Mutagenesis to Study GPCRs -- Approaches to Study GPCR Regulation in Native Systems -- Heterologous Expression of GPCRs in Fission Yeast -- Radioligand Binding Methods for Membrane Preparations and Intact Cells -- Quantification of GPCR mRNA Using Real-Time RT-PCR -- Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data -- Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells -- Examining Site-Specific GPCR Phosphorylation -- Ubiquitination of GPCRs -- [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs -- Using Calcium Imaging as a Readout of GPCR Activation -- Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors -- Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins -- The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events -- Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Study of GPCR-Protein Interactions by BRET -- Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization -- Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes -- Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes -- Identification of GPCR Localization in Detergent Resistant Membranes -- Analysis of GPCR Localization and Trafficking -- Statistical Methods in Research
Dimensions
unknown
Edition
3rd ed. 2011.
Extent
1 online resource (XI, 479p. 84 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781617791260
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-61779-126-0
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000084402
  • (SSID)ssj0000588085
  • (PQKBManifestationID)11363942
  • (PQKBTitleCode)TC0000588085
  • (PQKBWorkID)10646123
  • (PQKB)11030436
  • (DE-He213)978-1-61779-126-0
  • (EXLCZ)992670000000084402
Label
Receptor Signal Transduction Protocols : Third Edition, edited by Gary B. Willars, R.A. John Challiss, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Inducible Expression of G Protein-Coupled Receptors in Transfected Cells -- Using the Flp-InTM T-RexTM System to Regulate GPCR Expression -- Viral Infection for GPCR Expression in Eukaryotic Cells -- Generation of Epitope-Tagged GPCRs -- The Use of Site-Directed Mutagenesis to Study GPCRs -- Approaches to Study GPCR Regulation in Native Systems -- Heterologous Expression of GPCRs in Fission Yeast -- Radioligand Binding Methods for Membrane Preparations and Intact Cells -- Quantification of GPCR mRNA Using Real-Time RT-PCR -- Determining Allosteric Modulator Mechanism of Action: Integration of Radioligand Binding and Functional Assay Data -- Design and Use of Fluorescent Ligands to Study Ligand-Receptor Interactions in Single Living Cells -- Examining Site-Specific GPCR Phosphorylation -- Ubiquitination of GPCRs -- [35S]GTPγS Binding as an Index of Total G-Protein and Gα-Subtype-Specific Activation by GPCRs -- Using Calcium Imaging as a Readout of GPCR Activation -- Measuring Spatio-Temporal Dynamics of Cyclic AMP Signaling in Real-Time Using FRET-Based Biosensors -- Determining the Activation of Rho as an Index of Receptor Coupling to G12/13 Proteins -- The Use of Translocating Fluorescent Biosensors for Real-Time Monitoring of GPCR-Mediated Signaling Events -- Study of GPCR-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs -- Study of GPCR-Protein Interactions by BRET -- Time Resolved FRET Strategy with Fluorescent Ligands to Analyze Receptor Interactions in Native Tissues: Application to GPCR Oligomerization -- Peptide Affinity Purification for the Isolation and Identification of GPCR-Associated Protein Complexes -- Tandem Affinity Purification and Identification of GPCR-Associated Protein Complexes -- Identification of GPCR Localization in Detergent Resistant Membranes -- Analysis of GPCR Localization and Trafficking -- Statistical Methods in Research
Dimensions
unknown
Edition
3rd ed. 2011.
Extent
1 online resource (XI, 479p. 84 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781617791260
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-61779-126-0
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000084402
  • (SSID)ssj0000588085
  • (PQKBManifestationID)11363942
  • (PQKBTitleCode)TC0000588085
  • (PQKBWorkID)10646123
  • (PQKB)11030436
  • (DE-He213)978-1-61779-126-0
  • (EXLCZ)992670000000084402

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