The Resource Receptor Binding Techniques, edited by Mary Keen, (electronic resource)

Receptor Binding Techniques, edited by Mary Keen, (electronic resource)

Label
Receptor Binding Techniques
Title
Receptor Binding Techniques
Statement of responsibility
edited by Mary Keen
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
In Receptor Binding Techniques, detailed experimental protocols devised by expert practitioners demonstrate the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein-coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also take into account the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations. Receptor Binding Techniques shows clearly how these techniques can be adapted to many different applications and fully utilized in drug discovery programs. It will be of high value not only to researchers new to the field of receptor binding, but also to more experienced workers, serving as an authoritative bench manual replete with optimalized practical methods and protocols. Pharmacologists, neuroscientists, cell biologists, molecular biologists, and biochemists
Member of
Dewey number
571.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
cP-MfSZ-NbE
Image bit depth
0
Language note
English
LC call number
RM1-950
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Keen, Mary.
Series statement
Methods in Molecular Biology,
Series volume
106
http://library.link/vocab/subjectName
  • Toxicology
  • Pharmacology/Toxicology
Label
Receptor Binding Techniques, edited by Mary Keen, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Overview of Ligand-Receptor Binding Techniques -- Radioligand Binding in Different Preparations -- [3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes -- Characterization of Imidazoline Receptors by Radioligand Binding -- Radioligand Binding to Solubilized 5-HT3 Receptors -- Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization -- Radioligand Binding in Intact Cells -- Autoradiography of Peptide Receptors -- Measurement of Brain Gaba-Benzodiazepine Receptor Levels In Vivo Using Emission Tomography -- General Considerations -- Analysis of Binding Data -- Definition of Receptors in Radioligand-Binding Experiments -- The Use of Binding to Study Nonreceptor Sites -- Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels -- Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitors -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes -- mRNA: Detection by In Situ and Northern Hybridization
Dimensions
unknown
Edition
1st ed. 1999.
Extent
1 online resource (304 p.)
File format
multiple file formats
Form of item
online
Isbn
9781592595792
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1385/0896035301
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000240139
  • (SSID)ssj0000326172
  • (PQKBManifestationID)11242580
  • (PQKBTitleCode)TC0000326172
  • (PQKBWorkID)10296214
  • (PQKB)11662695
  • (DE-He213)978-1-59259-579-2
  • (EXLCZ)991000000000240139
Label
Receptor Binding Techniques, edited by Mary Keen, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Overview of Ligand-Receptor Binding Techniques -- Radioligand Binding in Different Preparations -- [3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes -- Characterization of Imidazoline Receptors by Radioligand Binding -- Radioligand Binding to Solubilized 5-HT3 Receptors -- Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization -- Radioligand Binding in Intact Cells -- Autoradiography of Peptide Receptors -- Measurement of Brain Gaba-Benzodiazepine Receptor Levels In Vivo Using Emission Tomography -- General Considerations -- Analysis of Binding Data -- Definition of Receptors in Radioligand-Binding Experiments -- The Use of Binding to Study Nonreceptor Sites -- Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels -- Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitors -- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes -- mRNA: Detection by In Situ and Northern Hybridization
Dimensions
unknown
Edition
1st ed. 1999.
Extent
1 online resource (304 p.)
File format
multiple file formats
Form of item
online
Isbn
9781592595792
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1385/0896035301
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000240139
  • (SSID)ssj0000326172
  • (PQKBManifestationID)11242580
  • (PQKBTitleCode)TC0000326172
  • (PQKBWorkID)10296214
  • (PQKB)11662695
  • (DE-He213)978-1-59259-579-2
  • (EXLCZ)991000000000240139

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