The Resource RT-PCR protocols, edited by Joe O'Connell, (electronic resource)

RT-PCR protocols, edited by Joe O'Connell, (electronic resource)

Label
RT-PCR protocols
Title
RT-PCR protocols
Statement of responsibility
edited by Joe O'Connell
Contributor
Subject
Genre
Language
eng
Summary
RT-PCR, the most widely used technique in biomedical research for analyzing gene expression, has proven a significant boon to the molecular investigation of disease pathogenesis. In RT-PCR Protocols, Joe O'Connell has assembled a panel of highly regarded molecular biologists and clinical researchers to describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Each protocol emphasizes the technical steps critical for experimental success and includes tips on avoiding potential pitfalls, notes explaining the method's scientific basis and its possible sources of error, and comments on how it might best be accommodated to various experimental circumstances. Diverse and wide-ranging, RT-PCR Protocols offers investigators powerful techniques to be used in the molecular investigation of disease pathogenesis and shows how RT-PCR can complement other technological advances in the design of today's new therapeutic strategies
Member of
Cataloging source
COO
Dewey number
572/.43
Illustrations
illustrations
Index
index present
LC call number
QP606.D46
LC item number
R8 2002
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 193
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QH 450.3
NLM item number
R893 2002
http://library.link/vocab/relatedWorkOrContributorName
O'Connell, Joe
Series statement
Methods in molecular biology
Series volume
v. 193
http://library.link/vocab/subjectName
  • Polymerase chain reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • SCIENCE
  • Polymerase chain reaction
Label
RT-PCR protocols, edited by Joe O'Connell, (electronic resource)
Link
http://dx.doi.org/10.1385/159259283X
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
RT-PCR in biomedicine. Opportunities arising from the new accessibility of mRNA / J. O'Connell -- The basics of RT-PCR. Some practical considerations / J. O'Connell -- Using the quantitative competitive RT-PCR technique to analyze minute amounts of different mRNAs in small tissue samples / S. Greber-Platzer [and others] -- Detection of mRNA expression and alteRNAtive splicing in a single cell / T. Kumazaki -- Nested RT-PCR. Sensitivity controls are essential to determine the biological significance of detected mRNA / T. Goode [and others] -- Quantitative RT-PCR. A review of current methodologies / C. Joyce -- Rapid development of a quantitative-competitive (qc) RT-PCR assay using a composite primer approach / J. O'Connell [and others] -- Quantitation of gene expression by RT-PCR and HPLC analysis of PCR products / F. Bachmair, C.G. Huber and G. Daxenbichler -- Time-resolved fluorometric detection of cytokine mRNAs amplified by RT-PCR / K. Nieminen [and others] -- Mimic-based RT-PCR quantitation of substance p mRNA in human mononuclear phagocytes and lymphocytes / J.P. Lai, S.D. Douglas and W.Z. Ho -- Detection and quantification of the hepatitis c viral genome / L.J. Fanning -- Semi-quantitative detection of hepatitis C virus RNA by "real-time" RT-PCR / J.F. Schlaak -- RT-PCR for the assessment of genetically heterogenous populations of the hepatitis C virus / B. Mullan [and others] -- In situ immuno-PCR. A newly developed method for highly sensitive antigen detection in situ / Y. Cao -- RT-PCR from laser-capture microdissected samples / T. Crnogorac-Jurcevic, T.O. Nielsen and N.R. Lemoine -- Mycobacterium paratuberculosis detected by nested PCR in intestinal granulomas isolated by LCM in cases of Crohn's disease / P. Ryan [and others] -- RT-PCR-based approaches to generate probes for mRNA detection by in situ hybridization / J. O'Connell -- Amplified RNA for gene array hybridizations / V.I. Shustova and S.J. Meltzer -- Semi-quantitative determination of differential gene expression in primary tumors and matched metastases by RT-PCR. Comparison with other methods / B. Mann and C. Hanski -- Detection of single nucleotide polymorphisms using a non-isotopic RNase cleavage assay / F. Waldron-Lynch [and others] -- Detection of clonally expanded T-cells by RT-PCR-SSCP and nucleotide sequencing of T-cell receptor [beta]-CDR3 regions / M.S. Kurokawa, K. Nishioka and T. Kato -- Generation of scFv from a phage display mini-library derived from tumor-infiltrating b-cells / N. Gruel [and others] -- Generation of murine SCFV intrabodies from B-cell hybridomas / C.H. Nam, S. Moutel and J.L. Teillaud -- Quantitation of mRNA levels by RT-PCR in cells purified by FACS. Application to peripheral cannabinoid receptors in leukocyte subsets / J. Marchand and P. Carayon -- Detection of anti-sense RNA transcripts by anti-sense RT-PCR / M.C. Yeung and A.S. Lau -- RT-PCR in cDNA library construction / V. Healy -- An RT-PCR-based protocol for the rapid generation of large, representative cDNA libraries for expression screening / J. O'Connell
Dimensions
unknown
Extent
1 online resource (xiv, 378 pages)
Form of item
online
Isbn
9780896038752
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)51979317
  • (OCoLC)ocm51979317
Label
RT-PCR protocols, edited by Joe O'Connell, (electronic resource)
Link
http://dx.doi.org/10.1385/159259283X
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
RT-PCR in biomedicine. Opportunities arising from the new accessibility of mRNA / J. O'Connell -- The basics of RT-PCR. Some practical considerations / J. O'Connell -- Using the quantitative competitive RT-PCR technique to analyze minute amounts of different mRNAs in small tissue samples / S. Greber-Platzer [and others] -- Detection of mRNA expression and alteRNAtive splicing in a single cell / T. Kumazaki -- Nested RT-PCR. Sensitivity controls are essential to determine the biological significance of detected mRNA / T. Goode [and others] -- Quantitative RT-PCR. A review of current methodologies / C. Joyce -- Rapid development of a quantitative-competitive (qc) RT-PCR assay using a composite primer approach / J. O'Connell [and others] -- Quantitation of gene expression by RT-PCR and HPLC analysis of PCR products / F. Bachmair, C.G. Huber and G. Daxenbichler -- Time-resolved fluorometric detection of cytokine mRNAs amplified by RT-PCR / K. Nieminen [and others] -- Mimic-based RT-PCR quantitation of substance p mRNA in human mononuclear phagocytes and lymphocytes / J.P. Lai, S.D. Douglas and W.Z. Ho -- Detection and quantification of the hepatitis c viral genome / L.J. Fanning -- Semi-quantitative detection of hepatitis C virus RNA by "real-time" RT-PCR / J.F. Schlaak -- RT-PCR for the assessment of genetically heterogenous populations of the hepatitis C virus / B. Mullan [and others] -- In situ immuno-PCR. A newly developed method for highly sensitive antigen detection in situ / Y. Cao -- RT-PCR from laser-capture microdissected samples / T. Crnogorac-Jurcevic, T.O. Nielsen and N.R. Lemoine -- Mycobacterium paratuberculosis detected by nested PCR in intestinal granulomas isolated by LCM in cases of Crohn's disease / P. Ryan [and others] -- RT-PCR-based approaches to generate probes for mRNA detection by in situ hybridization / J. O'Connell -- Amplified RNA for gene array hybridizations / V.I. Shustova and S.J. Meltzer -- Semi-quantitative determination of differential gene expression in primary tumors and matched metastases by RT-PCR. Comparison with other methods / B. Mann and C. Hanski -- Detection of single nucleotide polymorphisms using a non-isotopic RNase cleavage assay / F. Waldron-Lynch [and others] -- Detection of clonally expanded T-cells by RT-PCR-SSCP and nucleotide sequencing of T-cell receptor [beta]-CDR3 regions / M.S. Kurokawa, K. Nishioka and T. Kato -- Generation of scFv from a phage display mini-library derived from tumor-infiltrating b-cells / N. Gruel [and others] -- Generation of murine SCFV intrabodies from B-cell hybridomas / C.H. Nam, S. Moutel and J.L. Teillaud -- Quantitation of mRNA levels by RT-PCR in cells purified by FACS. Application to peripheral cannabinoid receptors in leukocyte subsets / J. Marchand and P. Carayon -- Detection of anti-sense RNA transcripts by anti-sense RT-PCR / M.C. Yeung and A.S. Lau -- RT-PCR in cDNA library construction / V. Healy -- An RT-PCR-based protocol for the rapid generation of large, representative cDNA libraries for expression screening / J. O'Connell
Dimensions
unknown
Extent
1 online resource (xiv, 378 pages)
Form of item
online
Isbn
9780896038752
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)51979317
  • (OCoLC)ocm51979317

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