The Resource Protocols in molecular parasitology, edited by John E. Hyde, (electronic resource)

Protocols in molecular parasitology, edited by John E. Hyde, (electronic resource)

Label
Protocols in molecular parasitology
Title
Protocols in molecular parasitology
Statement of responsibility
edited by John E. Hyde
Contributor
Subject
Genre
Language
eng
Summary
Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation
Member of
Cataloging source
YNG
Dewey number
574.5/249/0724
Illustrations
illustrations
Index
index present
LC call number
QL757
LC item number
.P79 1993
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QX 25
NLM item number
  • ME9616J v.21 1993
  • P967 1993
http://library.link/vocab/relatedWorkOrContributorName
Hyde, John E
Series statement
Methods in molecular biology
Series volume
v. 21
http://library.link/vocab/subjectName
  • Molecular parasitology
  • Parasitology
  • Parasites
  • Molecular Biology
  • Parasites
  • Molecular parasitology
Target audience
adult
Label
Protocols in molecular parasitology, edited by John E. Hyde, (electronic resource)
Link
http://dx.doi.org/10.1385/0896032396
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum -- Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries -- Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- Immunoblotting and enzyme linked immunosorbent assay -- Immunofluorescence of parasites -- Immunoelectron microscopic localization of antigens in malaria parasites -- Preparation of blotted membrane for protein microsequencing -- Identification of T-cell epitopes
Dimensions
unknown
Extent
1 online resource (xiv, 470 pages)
File format
one file format
Form of item
online
Isbn
9781592595082
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
  • (OCoLC)260242061
  • (OCoLC)ocn260242061
Label
Protocols in molecular parasitology, edited by John E. Hyde, (electronic resource)
Link
http://dx.doi.org/10.1385/0896032396
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum -- Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries -- Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- Immunoblotting and enzyme linked immunosorbent assay -- Immunofluorescence of parasites -- Immunoelectron microscopic localization of antigens in malaria parasites -- Preparation of blotted membrane for protein microsequencing -- Identification of T-cell epitopes
Dimensions
unknown
Extent
1 online resource (xiv, 470 pages)
File format
one file format
Form of item
online
Isbn
9781592595082
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Quality assurance targets
unknown
Reformatting quality
access
Specific material designation
remote
System control number
  • (OCoLC)260242061
  • (OCoLC)ocn260242061

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