The Resource Protein sequencing protocols, edited by Bryan John Smith, (electronic resource)

Protein sequencing protocols, edited by Bryan John Smith, (electronic resource)

Label
Protein sequencing protocols
Title
Protein sequencing protocols
Statement of responsibility
edited by Bryan John Smith
Contributor
Subject
Genre
Language
eng
Member of
Action
digitized
Cataloging source
COO
Dewey number
572.8/633
Illustrations
illustrations
Index
index present
LC call number
QP625.N89
LC item number
P76 2002
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 211
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.211 2003
  • P9675 2003
http://library.link/vocab/relatedWorkOrContributorName
Smith, Bryan John
Series statement
Methods in molecular biology
Series volume
v. 211
http://library.link/vocab/subjectName
  • Nucleotide sequence
  • Séquence nucléotidique
  • SCIENCE
  • Nucleotide sequence
  • Sequence Analysis, Protein
  • Proteins
Label
Protein sequencing protocols, edited by Bryan John Smith, (electronic resource)
Link
http://dx.doi.org/10.1385/1592593429
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J.C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xii, 493 pages)
Form of item
online
Isbn
9780896039759
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)170652685
  • (OCoLC)ocn170652685
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Protein sequencing protocols, edited by Bryan John Smith, (electronic resource)
Link
http://dx.doi.org/10.1385/1592593429
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J.C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xii, 493 pages)
Form of item
online
Isbn
9780896039759
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)170652685
  • (OCoLC)ocn170652685
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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