The Resource Protein kinase protocols, edited by Alastair D. Reith, (electronic resource)

Protein kinase protocols, edited by Alastair D. Reith, (electronic resource)

Label
Protein kinase protocols
Title
Protein kinase protocols
Statement of responsibility
edited by Alastair D. Reith
Contributor
Subject
Genre
Language
eng
Summary
As key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the protocols to work in related systems. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets
Member of
Cataloging source
COO
Dewey number
572/.76
Illustrations
illustrations
Index
index present
LC call number
QP606.P76
LC item number
.P737 2001
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 124
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QU 25
NLM item number
  • ME9616J v.124 2001
  • P9666 2001
http://library.link/vocab/relatedWorkOrContributorName
Reith, Alastair D
Series statement
Methods in molecular biology
Series volume
v. 124
http://library.link/vocab/subjectName
  • Protein kinases
  • Protéine kinase
  • SCIENCE
  • Wetenschappelijke technieken
  • Protein kinases
  • Protein Kinases
Label
Protein kinase protocols, edited by Alastair D. Reith, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590594
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Two-dimensional phosphopeptide mapping of receptor tyrosine kinases
  • Jeremy M. Tavar[226]e, Tarik Issad
  • Identification of the sites of phosphorylation in proteins using high performance liquid chromatography and mass spectrometry
  • A. Grey Craig
  • Phosphorylation of small signaling proteins by receptor serine/threonine kinases
  • Serhiy Souchelnytskyi [and others]
  • Assays for mitogen-activated protein kinases (MAPK) subtypes and MAPK activating protein kinase-2 (MAPKAP K-2) using a common cell lysate
  • Rozen Le Panse, Navita Rampersaud, Louis C. Mahadevan
  • JAK-mediated phosphorylation and activation of STAT signaling proteins: analysis by phosphotyrosine blotting and EMSA
  • Nicola Broughton, Mark S. Burfoot
  • Protein kinase-mediated signaling networks : regulation and functional characterization
  • Assays for glycogen synthase kinase-3 (GSK-3)
  • Darren Cross
  • Cyclin-dependent kinases and cyclin-dependent kinase inhibitors: detection methods and activity measurements
  • Gavom Brooks
  • Protein histidine kinase
  • Harry R. Matthews, Karina Chan
  • Cloning and expression of recombinant HEXA-HIS tagged ZAP-70 using the baculovirus system
  • Tracey Purton, Sandra Wilkinson, Edward J. Murray
  • Analysis of protein kinase subcellular localization by visualization of GTF fusion proteins
  • Ralph A. Zirngibi, Peter Greer
  • Alastair D. Reith
  • Detection of phosphorylation-dependent interactions by far-western gel overlay
  • Mary Rose Burnham, Regina DeBerry, Amy H. Bouton
  • Purification of tyrosine-phorylated proteins by immunoaffinity chromatography and direct cloning of their DNAs from bacterial expression libraries
  • Pier Paolo Di Fiore, Francesca Fazidi
  • Identification of receptor tyrosine kinase (RTK) substrates by the cloning of receptor targets (CORT) strategy
  • Roger J. Daley
  • Identification of receptor tyrosine kinase associating proteins using the yeast two-hybrid system
  • Sally A. Prigent
  • cDNA expression cloning and characterization of phosphorylation dependent protein interactors using the yeast tribid system
  • Christoph Volpers [and others] -- tAnalysis of SH2 domain -- phosphopeptide interactions by isothermal titration calorimetry and surface plasmon resonance
  • Cloning protein tyrosine kinases by screening cDNA libraries with antiphosphotyrosine antibodies
  • George Panayotou, John Ladbury
  • Cloning and characterization of RTK ligands using receptor-alkaline phosphatase fusion proteins
  • Hwai-Jong Cheng, John G. Flanagan
  • Isolation and characterization of "orphan-RTK" ligands using an integrated biosensor approach
  • Martin Lackmann
  • Lisa D. Chong, Ira O. Daar
  • Immunoprecipitation and western blotting of phosphotyrosine-containing proteins
  • Kathleen M. Woods Ignatoski
  • Two-dimensional phosphoamino acid analysis
  • Peter Blume-Jensen, Tony Hunter
Dimensions
unknown
Extent
1 online resource (xi, 365 pages)
Form of item
online
Isbn
9780896037007
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)170922021
  • (OCoLC)ocn170922021
Label
Protein kinase protocols, edited by Alastair D. Reith, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590594
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Two-dimensional phosphopeptide mapping of receptor tyrosine kinases
  • Jeremy M. Tavar[226]e, Tarik Issad
  • Identification of the sites of phosphorylation in proteins using high performance liquid chromatography and mass spectrometry
  • A. Grey Craig
  • Phosphorylation of small signaling proteins by receptor serine/threonine kinases
  • Serhiy Souchelnytskyi [and others]
  • Assays for mitogen-activated protein kinases (MAPK) subtypes and MAPK activating protein kinase-2 (MAPKAP K-2) using a common cell lysate
  • Rozen Le Panse, Navita Rampersaud, Louis C. Mahadevan
  • JAK-mediated phosphorylation and activation of STAT signaling proteins: analysis by phosphotyrosine blotting and EMSA
  • Nicola Broughton, Mark S. Burfoot
  • Protein kinase-mediated signaling networks : regulation and functional characterization
  • Assays for glycogen synthase kinase-3 (GSK-3)
  • Darren Cross
  • Cyclin-dependent kinases and cyclin-dependent kinase inhibitors: detection methods and activity measurements
  • Gavom Brooks
  • Protein histidine kinase
  • Harry R. Matthews, Karina Chan
  • Cloning and expression of recombinant HEXA-HIS tagged ZAP-70 using the baculovirus system
  • Tracey Purton, Sandra Wilkinson, Edward J. Murray
  • Analysis of protein kinase subcellular localization by visualization of GTF fusion proteins
  • Ralph A. Zirngibi, Peter Greer
  • Alastair D. Reith
  • Detection of phosphorylation-dependent interactions by far-western gel overlay
  • Mary Rose Burnham, Regina DeBerry, Amy H. Bouton
  • Purification of tyrosine-phorylated proteins by immunoaffinity chromatography and direct cloning of their DNAs from bacterial expression libraries
  • Pier Paolo Di Fiore, Francesca Fazidi
  • Identification of receptor tyrosine kinase (RTK) substrates by the cloning of receptor targets (CORT) strategy
  • Roger J. Daley
  • Identification of receptor tyrosine kinase associating proteins using the yeast two-hybrid system
  • Sally A. Prigent
  • cDNA expression cloning and characterization of phosphorylation dependent protein interactors using the yeast tribid system
  • Christoph Volpers [and others] -- tAnalysis of SH2 domain -- phosphopeptide interactions by isothermal titration calorimetry and surface plasmon resonance
  • Cloning protein tyrosine kinases by screening cDNA libraries with antiphosphotyrosine antibodies
  • George Panayotou, John Ladbury
  • Cloning and characterization of RTK ligands using receptor-alkaline phosphatase fusion proteins
  • Hwai-Jong Cheng, John G. Flanagan
  • Isolation and characterization of "orphan-RTK" ligands using an integrated biosensor approach
  • Martin Lackmann
  • Lisa D. Chong, Ira O. Daar
  • Immunoprecipitation and western blotting of phosphotyrosine-containing proteins
  • Kathleen M. Woods Ignatoski
  • Two-dimensional phosphoamino acid analysis
  • Peter Blume-Jensen, Tony Hunter
Dimensions
unknown
Extent
1 online resource (xi, 365 pages)
Form of item
online
Isbn
9780896037007
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)170922021
  • (OCoLC)ocn170922021

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