The Resource Protein analysis and purification : benchtop techniques, Ian M. Rosenberg, (electronic resource)

Protein analysis and purification : benchtop techniques, Ian M. Rosenberg, (electronic resource)

Label
Protein analysis and purification : benchtop techniques
Title
Protein analysis and purification
Title remainder
benchtop techniques
Statement of responsibility
Ian M. Rosenberg
Creator
Subject
Genre
Language
eng
Summary
New technologies and the manner in which scientific research is conducted have been fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS AND PURIFICATION. Among the many topics introduced to this new edition are the use of robotics in the lab, the impact of the codification of the human genome, functional proteomics, mass spectrometry, and the expanding use of bioinformatics as a tool to improve efficiency and productivity. The author has also included an annotated appendix of the educational materials and technical databases available online to enhance the researcher's benchside access to the latest resources. With solid explanations of the science asking the "why" and literally dozens of step-by-step protocols providing the "how," this new edition is a complete resource for the benchside
Cataloging source
COO
http://library.link/vocab/creatorDate
1949-
http://library.link/vocab/creatorName
Rosenberg, Ian M.
Dewey number
572/.6
Illustrations
illustrations
Index
index present
LC call number
QP551
LC item number
.R785 2004
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • 2004 M-606
  • QU 25
NLM item number
R813p 2005
http://library.link/vocab/subjectName
  • Proteins
  • Proteins
  • Proteins
  • Proteins
  • Protéines
  • Protéines
  • Biomédecine
  • Sciences de la vie
  • Proteins
  • Proteins
  • Proteínas (análise)
  • Proteine
  • Proteine
  • Trennverfahren
Label
Protein analysis and purification : benchtop techniques, Ian M. Rosenberg, (electronic resource)
Link
http://dx.doi.org/10.1007/b138330
Instantiates
Publication
Note
Originally published: Boston : Birkhäuser, ©1996
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1 An Overview of This Manual -- Kits, Cores, and Computers -- How to Use This Book -- Basic Laboratory Equipment -- Laboratory Automation -- Beyond Protein Analysis and Purification -- 2 Protein Structure -- A. The Amino Acids -- B. The Four Levels of Protein Structure -- C. Chemical Characteristics of Proteins -- 3 Tracking the Target Protein -- A. Labeling Cells and Proteins -- B. LysisPreparation of the Cell Free Extract -- C. Principles of Immunoprecipitation -- D. Additional Methods to Identify Associated Proteins -- 4 Electrophoretic Techniques -- to Polyacrylamide Gel Electrophoresis (PAGE) -- A. Preparation of SDS-Polyacrylamide Gels -- B. 2-Dimensional (2-D) Gel Systems -- D. Recovery of Proteins from the Gel -- E. Identification of Enzyme Activity in Polyacrylamide Gels -- 5 Getting Started with Protein Purification -- A. Making a Cell Free Extract -- B. Protein Quantitation -- C. Manipulating Proteins in Solution -- D. Precipitation Techniques -- 6 Membrane Proteins -- A. Peripheral Membrane Proteins -- B. Integral Membrane Proteins -- C. Detergents -- 7 Transfer and Detection of Proteins on -- Membrane Supports -- A. Transfer of Proteins to Membrane Supports -- B. Staining the Blot -- C. Recovery of Proteins from the Blot -- D. Detection of the Target Protein -- 8 Identification of the Target Protein -- A. Peptide Mapping -- B. Enzymatic Cleavage of Proteins -- C. Chemical Cleavage -- D. Microsequencing from PVDF Membranes -- MS Basics -- Protein Identification by MS -- Protein Database Searches -- The Rise of Biological Databases -- 9 Identifying and Analyzing Posttranslational -- Modifications -- A. Glycosylation -- B. Phosphorylation -- C. Lipid Modification of Proteins -- D. Selected Modifications -- 10 Chromatography -- A. Important Terminology Used in Chromatography -- B. Gel Filtration Chromatography -- C. Introduction to HPLC -- D. Ion Exchange Chromatography: Separation on the Basis of Charge -- E. Hydrophobic Interaction Chromatography (HIC) -- Flow Rate -- 11 Recombinant Protein Techniques -- Recombinant Protein for Antibody Production -- Protein for Biochemical or Cell Biological Studies -- A. In vitro Transcription and Translation -- B. Recombinant Gene Products in E. coli: Expression, Identification and Characterization -- D. Expression of Foreign Proteins in Eukaryotic Cells -- Appendices -- A. Safety Considerations -- First Aid: Emergency Procedures -- B. Antibody Preparation -- Production of Polyclonal Antisera in Rabbits -- Purification of Antibody Using Protein A Affinity Columns -- Numbering Mice -- Molarity -- Choosing and Preparing Buffers -- Common Laboratory Solutions -- Extinction Coefficients -- D. Nucleic Acids -- Spectrophotometric Conversions -- DNA/Protein Conversions -- Oligonucleotide Concentrations -- RNA Precipitation -- E. Modifications and Motifs -- Nomenclature -- Protein Modification Sequences -- Protein Kinase Recognition Sequence Motifs -- Subcellular Localization Motifs -- Protein Databases -- F. Centrifugation -- Nomogram -- General Purpose Centrifuge Rotors -- Ultracentrifuge Rotors -- G. Proteases and Proteolytic Enzyme Inhibitors -- Commonly Used Proteases -- Protease Inhibitors -- H. Radioactivity -- Manual and Machine Film Processing -- L Tissue Culture -- Transwell Permeable Supports -- J. Miscellaneous -- Unit Prefixes -- The Greek Alphabet -- Abbreviations -- HPLC Pump Pressure Conversion -- Dipeptide Masses -- Mass Differences Considered in Molecular Weight Analysis of Proteins -- K. List of Suppliers, Vendors, Manufacturers
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xxvi, 520 pages)
Form of item
online
Isbn
9781461220565
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Stock number
133573
System control number
  • (OCoLC)288195462
  • (OCoLC)ocn288195462
Label
Protein analysis and purification : benchtop techniques, Ian M. Rosenberg, (electronic resource)
Link
http://dx.doi.org/10.1007/b138330
Publication
Note
Originally published: Boston : Birkhäuser, ©1996
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
1 An Overview of This Manual -- Kits, Cores, and Computers -- How to Use This Book -- Basic Laboratory Equipment -- Laboratory Automation -- Beyond Protein Analysis and Purification -- 2 Protein Structure -- A. The Amino Acids -- B. The Four Levels of Protein Structure -- C. Chemical Characteristics of Proteins -- 3 Tracking the Target Protein -- A. Labeling Cells and Proteins -- B. LysisPreparation of the Cell Free Extract -- C. Principles of Immunoprecipitation -- D. Additional Methods to Identify Associated Proteins -- 4 Electrophoretic Techniques -- to Polyacrylamide Gel Electrophoresis (PAGE) -- A. Preparation of SDS-Polyacrylamide Gels -- B. 2-Dimensional (2-D) Gel Systems -- D. Recovery of Proteins from the Gel -- E. Identification of Enzyme Activity in Polyacrylamide Gels -- 5 Getting Started with Protein Purification -- A. Making a Cell Free Extract -- B. Protein Quantitation -- C. Manipulating Proteins in Solution -- D. Precipitation Techniques -- 6 Membrane Proteins -- A. Peripheral Membrane Proteins -- B. Integral Membrane Proteins -- C. Detergents -- 7 Transfer and Detection of Proteins on -- Membrane Supports -- A. Transfer of Proteins to Membrane Supports -- B. Staining the Blot -- C. Recovery of Proteins from the Blot -- D. Detection of the Target Protein -- 8 Identification of the Target Protein -- A. Peptide Mapping -- B. Enzymatic Cleavage of Proteins -- C. Chemical Cleavage -- D. Microsequencing from PVDF Membranes -- MS Basics -- Protein Identification by MS -- Protein Database Searches -- The Rise of Biological Databases -- 9 Identifying and Analyzing Posttranslational -- Modifications -- A. Glycosylation -- B. Phosphorylation -- C. Lipid Modification of Proteins -- D. Selected Modifications -- 10 Chromatography -- A. Important Terminology Used in Chromatography -- B. Gel Filtration Chromatography -- C. Introduction to HPLC -- D. Ion Exchange Chromatography: Separation on the Basis of Charge -- E. Hydrophobic Interaction Chromatography (HIC) -- Flow Rate -- 11 Recombinant Protein Techniques -- Recombinant Protein for Antibody Production -- Protein for Biochemical or Cell Biological Studies -- A. In vitro Transcription and Translation -- B. Recombinant Gene Products in E. coli: Expression, Identification and Characterization -- D. Expression of Foreign Proteins in Eukaryotic Cells -- Appendices -- A. Safety Considerations -- First Aid: Emergency Procedures -- B. Antibody Preparation -- Production of Polyclonal Antisera in Rabbits -- Purification of Antibody Using Protein A Affinity Columns -- Numbering Mice -- Molarity -- Choosing and Preparing Buffers -- Common Laboratory Solutions -- Extinction Coefficients -- D. Nucleic Acids -- Spectrophotometric Conversions -- DNA/Protein Conversions -- Oligonucleotide Concentrations -- RNA Precipitation -- E. Modifications and Motifs -- Nomenclature -- Protein Modification Sequences -- Protein Kinase Recognition Sequence Motifs -- Subcellular Localization Motifs -- Protein Databases -- F. Centrifugation -- Nomogram -- General Purpose Centrifuge Rotors -- Ultracentrifuge Rotors -- G. Proteases and Proteolytic Enzyme Inhibitors -- Commonly Used Proteases -- Protease Inhibitors -- H. Radioactivity -- Manual and Machine Film Processing -- L Tissue Culture -- Transwell Permeable Supports -- J. Miscellaneous -- Unit Prefixes -- The Greek Alphabet -- Abbreviations -- HPLC Pump Pressure Conversion -- Dipeptide Masses -- Mass Differences Considered in Molecular Weight Analysis of Proteins -- K. List of Suppliers, Vendors, Manufacturers
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xxvi, 520 pages)
Form of item
online
Isbn
9781461220565
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations
Specific material designation
remote
Stock number
133573
System control number
  • (OCoLC)288195462
  • (OCoLC)ocn288195462

Library Locations

  • Architecture LibraryBorrow it
    Gould Hall 830 Van Vleet Oval Rm. 105, Norman, OK, 73019, US
    35.205706 -97.445050
  • Bizzell Memorial LibraryBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Boorstin CollectionBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Chinese Literature Translation ArchiveBorrow it
    401 W. Brooks St., RM 414, Norman, OK, 73019, US
    35.207487 -97.447906
  • Engineering LibraryBorrow it
    Felgar Hall 865 Asp Avenue, Rm. 222, Norman, OK, 73019, US
    35.205706 -97.445050
  • Fine Arts LibraryBorrow it
    Catlett Music Center 500 West Boyd Street, Rm. 20, Norman, OK, 73019, US
    35.210371 -97.448244
  • Harry W. Bass Business History CollectionBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • History of Science CollectionsBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • John and Mary Nichols Rare Books and Special CollectionsBorrow it
    401 W. Brooks St., Rm. 509NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • Library Service CenterBorrow it
    2601 Technology Place, Norman, OK, 73019, US
    35.185561 -97.398361
  • Price College Digital LibraryBorrow it
    Adams Hall 102 307 West Brooks St., Norman, OK, 73019, US
    35.210371 -97.448244
  • Western History CollectionsBorrow it
    Monnet Hall 630 Parrington Oval, Rm. 300, Norman, OK, 73019, US
    35.209584 -97.445414
Processing Feedback ...