The Resource Protein Electrophoresis : Methods and Protocols, edited by Biji T. Kurien, R. Hal Scofield, (electronic resource)

Protein Electrophoresis : Methods and Protocols, edited by Biji T. Kurien, R. Hal Scofield, (electronic resource)

Label
Protein Electrophoresis : Methods and Protocols
Title
Protein Electrophoresis
Title remainder
Methods and Protocols
Statement of responsibility
edited by Biji T. Kurien, R. Hal Scofield
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field
Member of
Dewey number
572.6
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • WJCy2EiUz3g
  • igVtxHl0qsQ
Image bit depth
0
Language note
English
LC call number
QD431-431.7
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Kurien, Biji T.
  • Scofield, R. Hal.
Series statement
Methods in Molecular Biology,
http://library.link/vocab/subjectName
  • Biochemistry
  • Proteomics
  • Electrophoresis
  • Protein Science
  • Proteomics
  • Electrophoresis
Label
Protein Electrophoresis : Methods and Protocols, edited by Biji T. Kurien, R. Hal Scofield, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
How It All Began -- A Personal History of Gel-Electrophoresis -- Introduction to Protein Electrophoresis -- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis -- Protein Solubilization: Attend to the Choice of Lysis Buffer -- Native Polyacrylamide Gels -- Cationic Electrophoresis -- SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins -- Tricine-SDS-PAGE -- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis -- Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins -- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis -- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples -- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies -- Microchip Electrophoresis, with respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease” -- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols -- Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE -- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass -- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting -- Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing -- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids -- Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution -- Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE -- One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis -- Two-Dimensional Difference Gel Electrophoresis -- Protein Identification on Archived 2-D Gels -- Diagonal Electrophoresis for the Detection of Protein Disulfides -- Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes -- Immunoelectrophoresis: A Method with Many Faces -- Cell Surface Protein Biotinylation for SDS PAGE Analysis -- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization -- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome -- Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test -- Extraction of Proteins from Gels - A Brief Review -- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets -- Isolation of Proteins from Polyacrylamide Gels -- Gel Drying Methods -- Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags -- A Brief Review of Other Notable Electrophoretic Methods -- Protein Stains and Applications -- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels -- Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat -- Silver Staining Techniques of Polyacrylamide Gels -- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis -- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry -- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE -- Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins -- Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping -- Fluorescent Staining of Gels -- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis -- Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain -- Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain -- In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin -- Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black -- Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels -- Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner -- A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels -- Ultra-Sensitive Protein Detection and Imaging: Comparison of LumiteinTM, ProteoSilverTM, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains -- Common Artifacts and Mistakes Made in Electrophoresis
Dimensions
unknown
Edition
1st ed. 2012.
Extent
1 online resource (XIV, 648 p. 151 illus., 31 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781617798214
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-61779-821-4
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3390000000031654
  • (SSID)ssj0000697596
  • (PQKBManifestationID)11426631
  • (PQKBTitleCode)TC0000697596
  • (PQKBWorkID)10692437
  • (PQKB)10515924
  • (DE-He213)978-1-61779-821-4
  • (EXLCZ)993390000000031654
Label
Protein Electrophoresis : Methods and Protocols, edited by Biji T. Kurien, R. Hal Scofield, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
How It All Began -- A Personal History of Gel-Electrophoresis -- Introduction to Protein Electrophoresis -- Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis -- Protein Solubilization: Attend to the Choice of Lysis Buffer -- Native Polyacrylamide Gels -- Cationic Electrophoresis -- SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins -- Tricine-SDS-PAGE -- Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis -- Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins -- Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis -- A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples -- Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies -- Microchip Electrophoresis, with respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease” -- A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols -- Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE -- Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass -- SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting -- Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing -- Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids -- Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution -- Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE -- One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis -- Two-Dimensional Difference Gel Electrophoresis -- Protein Identification on Archived 2-D Gels -- Diagonal Electrophoresis for the Detection of Protein Disulfides -- Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes -- Immunoelectrophoresis: A Method with Many Faces -- Cell Surface Protein Biotinylation for SDS PAGE Analysis -- A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization -- Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome -- Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test -- Extraction of Proteins from Gels - A Brief Review -- A New Algorithm for the Simulation of SDS 2D-PAGE Datasets -- Isolation of Proteins from Polyacrylamide Gels -- Gel Drying Methods -- Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags -- A Brief Review of Other Notable Electrophoretic Methods -- Protein Stains and Applications -- Coomassie-Brilliant Blue Staining of Polyacrylamide Gels -- Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat -- Silver Staining Techniques of Polyacrylamide Gels -- Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis -- Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry -- TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE -- Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins -- Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping -- Fluorescent Staining of Gels -- A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis -- Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain -- Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain -- In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin -- Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black -- Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels -- Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner -- A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels -- Ultra-Sensitive Protein Detection and Imaging: Comparison of LumiteinTM, ProteoSilverTM, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains -- Common Artifacts and Mistakes Made in Electrophoresis
Dimensions
unknown
Edition
1st ed. 2012.
Extent
1 online resource (XIV, 648 p. 151 illus., 31 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781617798214
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-61779-821-4
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3390000000031654
  • (SSID)ssj0000697596
  • (PQKBManifestationID)11426631
  • (PQKBTitleCode)TC0000697596
  • (PQKBWorkID)10692437
  • (PQKB)10515924
  • (DE-He213)978-1-61779-821-4
  • (EXLCZ)993390000000031654

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