The Resource Plant microRNAs : methods and protocols, edited by Blake C. Meyers and Pamela J. Green, (electronic resource)

Plant microRNAs : methods and protocols, edited by Blake C. Meyers and Pamela J. Green, (electronic resource)

Label
Plant microRNAs : methods and protocols
Title
Plant microRNAs
Title remainder
methods and protocols
Statement of responsibility
edited by Blake C. Meyers and Pamela J. Green
Contributor
Subject
Genre
Language
eng
Summary
MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike
Member of
Cataloging source
CUS
Dewey number
572.882
Illustrations
illustrations
Index
index present
LC call number
QK981.4
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 25
http://library.link/vocab/relatedWorkOrContributorName
  • Meyers, Blake C
  • Green, Pamela J
Series statement
  • Springer protocols
  • Methods in molecular biology,
Series volume
592
http://library.link/vocab/subjectName
  • Small interfering RNA
  • Plant molecular genetics
  • MicroRNAs
  • RNA, Small Interfering
  • Plants
Label
Plant microRNAs : methods and protocols, edited by Blake C. Meyers and Pamela J. Green, (electronic resource)
Link
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Piecing the puzzle together : genetic requirements for miRNA biogenesis in Arabidopsis thaliana / Zhixin Xie -- Prediction of plant miRNA genes / Matthew W. Jones-Rhoades -- Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species / Monica Accerbi [and others] -- miRNA target prediction in plants / Noah Fahlgren and James C. Carrington -- A method to discover phased siRNA loci / Michael J. Axtell -- Directed gene silencing with artificial microRNAs / Rebecca Schwab [and others] -- Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies / Kan Nobuta [and others] -- High-throughput approaches for miRNA expression analysis / Cheng Lu and Frédéric Souret -- In situ detection of miRNAs using LNA probes / Zoltán Havelda -- Analysis of miRNA modifications / Bin Yu and Xuemei Chen -- MicroRNA promoter analysis / Molly Megraw and Artemis G. Hatzigeorgiou -- Computational methods for comparative analysis of plant small RNAs / Gayathri Mahalingam and Blake C. Meyers -- Biotic stress-associated microRNAs : identification, detection, regulation, and functional analysis / Florence Jay [and others] -- Abiotic stress-associated miRNAs : detection and functional analysis / Dong-Hoon Jeong [and others] -- Processing of miRNA precursors / Yukio Kurihara and Yuichiro Watanabe -- Purification of Arabidopsis argonaute complexes and associated small RNAs / Yijun Qi and Shijun Mi -- Transient assays for the analysis of miRNA processing and function / Felipe F. de Felippes and Detlef Weigel
Dimensions
unknown
Extent
1 online resource (xi, 267 pages)
Form of item
online
Isbn
9781603270052
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)457241739
  • (OCoLC)ocn457241739
Label
Plant microRNAs : methods and protocols, edited by Blake C. Meyers and Pamela J. Green, (electronic resource)
Link
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Piecing the puzzle together : genetic requirements for miRNA biogenesis in Arabidopsis thaliana / Zhixin Xie -- Prediction of plant miRNA genes / Matthew W. Jones-Rhoades -- Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species / Monica Accerbi [and others] -- miRNA target prediction in plants / Noah Fahlgren and James C. Carrington -- A method to discover phased siRNA loci / Michael J. Axtell -- Directed gene silencing with artificial microRNAs / Rebecca Schwab [and others] -- Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies / Kan Nobuta [and others] -- High-throughput approaches for miRNA expression analysis / Cheng Lu and Frédéric Souret -- In situ detection of miRNAs using LNA probes / Zoltán Havelda -- Analysis of miRNA modifications / Bin Yu and Xuemei Chen -- MicroRNA promoter analysis / Molly Megraw and Artemis G. Hatzigeorgiou -- Computational methods for comparative analysis of plant small RNAs / Gayathri Mahalingam and Blake C. Meyers -- Biotic stress-associated microRNAs : identification, detection, regulation, and functional analysis / Florence Jay [and others] -- Abiotic stress-associated miRNAs : detection and functional analysis / Dong-Hoon Jeong [and others] -- Processing of miRNA precursors / Yukio Kurihara and Yuichiro Watanabe -- Purification of Arabidopsis argonaute complexes and associated small RNAs / Yijun Qi and Shijun Mi -- Transient assays for the analysis of miRNA processing and function / Felipe F. de Felippes and Detlef Weigel
Dimensions
unknown
Extent
1 online resource (xi, 267 pages)
Form of item
online
Isbn
9781603270052
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)457241739
  • (OCoLC)ocn457241739

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