The Resource Plant chemical genomics : methods and protocols, edited by Glenn R. Hicks, Stephanie Robert

Plant chemical genomics : methods and protocols, edited by Glenn R. Hicks, Stephanie Robert

Label
Plant chemical genomics : methods and protocols
Title
Plant chemical genomics
Title remainder
methods and protocols
Statement of responsibility
edited by Glenn R. Hicks, Stephanie Robert
Contributor
Editor
Subject
Genre
Language
eng
Summary
Plant Chemical Genomics: Methods and Protocols , expert researchers in the field detail many of the techniques used for identification of bioactive compounds from a large selection of fields in plant biology including plant pathogenesis, immune responses, small RNA processing, endomembrane trafficking, lipids, plant hormone signaling and cell wall. The presentation of these and other examples as well as synthetic chemistry, computation and target identification provides a comprehensive overview of the practical aspects of chemical biology that are possible in plant systems. Small molecules and natural products that produce a phenotype in plants have been known for decades. The agrichemical industry has utilized small molecules based on known natural molecules such as auxins, cytokinins, abscissic acid and other growth regulators. In recent years, the screening and characterization of novel small molecules has enhanced our understanding of protein function, metabolic, signaling and endomembrane pathways, and their interactions in basic plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Member of
Cataloging source
GW5XE
Dewey number
576.5
Illustrations
illustrations
Index
index present
LC call number
QK981
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Hicks, Glenn R.
  • Robert, Stephanie
Series statement
Methods in Molecular Biology
Series volume
1056
http://library.link/vocab/subjectName
  • Plant genetics
  • Plants
Label
Plant chemical genomics : methods and protocols, edited by Glenn R. Hicks, Stephanie Robert
Link
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Fully-automated Compound Screening in Arabidopsis thaliana Seedlings -- Time-profiling Fluorescent Reporters in the Arabidopsis Root -- Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana -- Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes -- High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses -- Using a Reverse Genetics Approach to Investigate Small Molecule Activity -- Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics -- Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta -- Forward Chemical Screening of Small RNA Pathways -- Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening -- High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen -- Plant Chemical Genomics: Gravity Sensing and Response -- Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole -- The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery -- Cheminformatic Analysis of High-Throughput Compound Screens -- Endomembrane Dissection Using Chemically-Induced Bioactive Clusters -- Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule Screening -- Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification -- Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System -- Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry -- 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments -- Determination of Auxin Transport Parameters on the Cellular Level -- Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters
Dimensions
unknown
Extent
1 online resource (xi, 269 pages)
File format
unknown
Form of item
online
Isbn
9781627035927
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-592-7
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4432269-01okla_normanlaw
  • (SIRSI)4432269
  • (Sirsi) o865092817
  • (OCoLC)865092817
Label
Plant chemical genomics : methods and protocols, edited by Glenn R. Hicks, Stephanie Robert
Link
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Fully-automated Compound Screening in Arabidopsis thaliana Seedlings -- Time-profiling Fluorescent Reporters in the Arabidopsis Root -- Screening for Bioactive Small Molecules by in vivo Monitoring of Luciferase-based Reporter Gene Expression in Arabidopsis thaliana -- Application of Yeast-two Hybrid Assay to Chemical Genomic Screens: A High-throughput System to Identify Novel Molecules Modulating Plant Hormone Receptor Complexes -- High-throughput Screening of Small Molecule Libraries for Inducers of Plant Defense Responses -- Using a Reverse Genetics Approach to Investigate Small Molecule Activity -- Investigating the Phytohormone Ethylene Response Pathway by Chemical Genetics -- Screening for Inhibitors of Chloroplast Galactolipid Synthesis Acting in membrano and in Planta -- Forward Chemical Screening of Small RNA Pathways -- Identification and Use of Fluorescent Dyes for Plant Cell Wall Imaging Using High-Throughput Screening -- High-throughput Identification of Chemical Endomembrane Cycling Disruptors Utilizing Tobacco Pollen -- Plant Chemical Genomics: Gravity Sensing and Response -- Screening Chemical Libraries for Compounds that Affect Protein Sorting to the Yeast Vacuole -- The use of Multi-drug Approach to Uncover New Players of the Endomembrane System Trafficking Machinery -- Cheminformatic Analysis of High-Throughput Compound Screens -- Endomembrane Dissection Using Chemically-Induced Bioactive Clusters -- Statistical Molecular Design: A Tool to Follow Up Hits From Small Molecule Screening -- Early Stage Hit Triage for Plant Chemical Genetic Screens and Target Site Identification -- Screening for Gene Function using the FOX (Full-length cDNA OvereXpressor gene) Hunting System -- Quantification of Stable Isotope Label in Metabolites via Mass Spectrometry -- 1H NMR Based Metabolomics Methods for Chemical Genomics Experiments -- Determination of Auxin Transport Parameters on the Cellular Level -- Analyzing the in vivo Status of Exogenously Applied Auxins: A HPLC-based Method to Characterize the Intracellularly Localized Auxin Transporters
Dimensions
unknown
Extent
1 online resource (xi, 269 pages)
File format
unknown
Form of item
online
Isbn
9781627035927
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-592-7
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4432269-01okla_normanlaw
  • (SIRSI)4432269
  • (Sirsi) o865092817
  • (OCoLC)865092817

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