The Resource Plant Reverse Genetics : Methods and Protocols, edited by Andy Pereira, (electronic resource)

Plant Reverse Genetics : Methods and Protocols, edited by Andy Pereira, (electronic resource)

Label
Plant Reverse Genetics : Methods and Protocols
Title
Plant Reverse Genetics
Title remainder
Methods and Protocols
Statement of responsibility
edited by Andy Pereira
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view
Member of
Is Subseries of
Dewey number
572.862
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
ZmLTeNBoarM
Image bit depth
0
Language note
English
LC call number
QK1-989
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
Pereira, Andy.
Series statement
Methods in Molecular Biology,
Series volume
678
http://library.link/vocab/subjectName
  • Botany
  • Plant genetics
  • Medical genetics
  • Plant Sciences
  • Plant Genetics and Genomics
  • Gene Function
Label
Plant Reverse Genetics : Methods and Protocols, edited by Andy Pereira, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Analysis of High-Throughput Sequencing Data -- Identification of Plant MicroRNAs Using Expressed Sequence Tag Analysis -- Microarray Data Analysis -- Setting Up Reverse Transcription Quantitative-PCR Experiments -- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species -- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses -- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System) -- Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis -- Activation Tagging and Insertional Mutagenesis in Barley -- Methods for Rice Phenomics Studies -- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice -- Transposon Insertional Mutagenesis in Rice -- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants -- Screening Arabidopsis Genotypes for Drought Stress Resistance -- Protein Tagging for Chromatin Immunoprecipitation (ChIP) from Arabidopsis -- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions -- Plant Metabolomics by GC-MS and Differential Analysis -- Gramene Database: A Hub for Comparative Plant Genomics
Dimensions
unknown
Edition
1st ed. 2011.
Extent
1 online resource (XI, 282 p. 63 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607616825
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-60761-682-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000057446
  • (SSID)ssj0000450110
  • (PQKBManifestationID)11282785
  • (PQKBTitleCode)TC0000450110
  • (PQKBWorkID)10433769
  • (PQKB)10532893
  • (DE-He213)978-1-60761-682-5
  • (EXLCZ)992670000000057446
Label
Plant Reverse Genetics : Methods and Protocols, edited by Andy Pereira, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
  • cr
Color
not applicable
Content category
text
Content type code
  • txt
Contents
Analysis of High-Throughput Sequencing Data -- Identification of Plant MicroRNAs Using Expressed Sequence Tag Analysis -- Microarray Data Analysis -- Setting Up Reverse Transcription Quantitative-PCR Experiments -- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species -- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses -- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System) -- Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis -- Activation Tagging and Insertional Mutagenesis in Barley -- Methods for Rice Phenomics Studies -- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice -- Transposon Insertional Mutagenesis in Rice -- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants -- Screening Arabidopsis Genotypes for Drought Stress Resistance -- Protein Tagging for Chromatin Immunoprecipitation (ChIP) from Arabidopsis -- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions -- Plant Metabolomics by GC-MS and Differential Analysis -- Gramene Database: A Hub for Comparative Plant Genomics
Dimensions
unknown
Edition
1st ed. 2011.
Extent
1 online resource (XI, 282 p. 63 illus., 2 illus. in color.)
File format
multiple file formats
Form of item
online
Isbn
9781607616825
Level of compression
uncompressed
Media category
computer
Media type code
  • c
Other control number
10.1007/978-1-60761-682-5
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)2670000000057446
  • (SSID)ssj0000450110
  • (PQKBManifestationID)11282785
  • (PQKBTitleCode)TC0000450110
  • (PQKBWorkID)10433769
  • (PQKB)10532893
  • (DE-He213)978-1-60761-682-5
  • (EXLCZ)992670000000057446

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