The Resource PCR cloning protocols, edited by Bing-Yuan Chen and Harry W. Janes, (electronic resource)

PCR cloning protocols, edited by Bing-Yuan Chen and Harry W. Janes, (electronic resource)

Label
PCR cloning protocols
Title
PCR cloning protocols
Statement of responsibility
edited by Bing-Yuan Chen and Harry W. Janes
Contributor
Subject
Genre
Language
eng
Summary
Annotation
Member of
Action
digitized
Cataloging source
N$T
Dewey number
572.8/6
Illustrations
illustrations
Index
index present
LC call number
QH442.2
LC item number
.P37 2002eb
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 192
Nature of contents
  • standards specifications
  • bibliography
NLM call number
  • W1
  • QH 450.3
NLM item number
  • ME9616J v.192 2002
  • P3473 2002
http://library.link/vocab/relatedWorkOrContributorName
  • Chen, Bing-Yuan
  • Janes, Harry W
Series statement
Methods in molecular biology
Series volume
192
http://library.link/vocab/subjectName
  • Molecular cloning
  • Polymerase chain reaction
  • Polymerase Chain Reaction
  • Cloning, Molecular
  • Clonamiento molecular
  • Clonage moléculaire
  • Réaction en chaîne de la polymérase
  • SCIENCE
  • Molecular cloning
  • Polymerase chain reaction
  • Polymerase kettingreactie
  • Genetische manipulatie
  • Wetenschappelijke technieken
  • Kloneren
Summary expansion
PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment
Label
PCR cloning protocols, edited by Bing-Yuan Chen and Harry W. Janes, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591779
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for PCR primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step PCR optimization using touchdown and stepdown PCR programming / K.H. Roux -- Xl PCR amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / J.T. Aatsinki -- Long distance reverse-transcription PCR / V. Thiel, J. Herold and S.G. Siddell -- Increasing PCR sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects / A. Moreau [and others] -- PCR procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific PCR / H. Ohashi -- Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning PCR products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable PCR products / K. Wang -- Enzyme-free cloning of PCR products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of PCR products into vectors / G.J. Chen -- Autosticky PCR. Directional cloning of PCR products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of PCR products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- PCR approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using PCR inserts / J.C. Pierce -- Megaprimer PCR / S. Barik -- PCR method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- PCR screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid PCR amplification / D. Kim and F.P. Guengerich -- PCR-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette PCR (ldv PCR) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse PCR. cDNA cloning / S.H. Huang -- Inverse PCR. Genomic DNA cloning / A.Y. Jong [and others] -- Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking TN5 transposon insertions by inverse PCR / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored PCR / Z.N. Yang and T.E. Mirkov -- A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of PCR in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture PCR / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by PCR / M.W. King -- Rapid cDNA cloning by PCR screening (RC-PCR) / T. Takumi -- Generation and PCR screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- PCR-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput PCR product purification method / R. Smith and K. Wang
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xiv, 439 pages)
Form of item
online
Isbn
9780585403342
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)50175114
  • (OCoLC)ocm50175114
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
PCR cloning protocols, edited by Bing-Yuan Chen and Harry W. Janes, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591779
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Polymerase chain reaction. Basic principles and routine practice / L.A. Kolmodin and D.E. Birch -- Computer programs for PCR primer design and analysis / B.Y. Chen, H.W. Janes and S. Chen -- Single-step PCR optimization using touchdown and stepdown PCR programming / K.H. Roux -- Xl PCR amplification of long targets from genomic DNA / L.A. Kolmodin -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / J.T. Aatsinki -- Long distance reverse-transcription PCR / V. Thiel, J. Herold and S.G. Siddell -- Increasing PCR sensitivity for amplification from paraffin-embedded tissues / A. Akalu and J.K. Reichardt -- GC-rich template amplification by inverse PCR. DNA polymerase and solvent effects / A. Moreau [and others] -- PCR procedure for the isolation of trinucleotide repeats / T. Tozaki -- Methylation-specific PCR / H. Ohashi -- Direct cloning of full-length cell differentially expressed genes by multiple rounds of subtractive hybridization based on long-distance PCR and magnetic beads / X. Huang, Z. Yuan and X. Cao -- Cloning PCR products. An overview / B. Guo and Y. Bi -- Using t4 DNA polymerase to generate clonable PCR products / K. Wang -- Enzyme-free cloning of PCR products and fusion protein expression / B.A. Neilan and D. Tillett -- Directional restriction site-free insertion of PCR products into vectors / G.J. Chen -- Autosticky PCR. Directional cloning of PCR products with performed 5' overhangs / J. Gal and M. Kalman -- A rapid and simple procedure for direct cloning of PCR products into baculoviruses / T.S. Gritsun, M.V. Mikhailov and E.A. Gould -- PCR approaches to DNA mutagenesis and recombination. An overview / B. Shen -- In-frame cloning of synthetic genes using PCR inserts / J.C. Pierce -- Megaprimer PCR / S. Barik -- PCR method for generating multiple mutations at adjacent sites / J. Adamec -- A fast polymerase chain reaction-mediated strategy for introducing repeat expansions into cag-repeat containing genes / F. Laccone -- PCR screening in signature-tagged mutagenesis of essential genes / D.E. Lehoux and R.C. Levesque -- Staggered extension process (step) in vitro recombination / A.M. Aguinaldo and F. Arnold -- Random mutagenesis by whole-plasmid PCR amplification / D. Kim and F.P. Guengerich -- PCR-based strategies to clone unknown DNA regions from known foreign integrants. An overview / E.K. Hui, P.C. Wang and S.J. Lo -- Long distance vectorette PCR (ldv PCR) / J.A. Fenton, G. Pratt and G.J. Morgan -- Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("cold-start method") / M. Lardelli -- Inverse PCR. cDNA cloning / S.H. Huang -- Inverse PCR. Genomic DNA cloning / A.Y. Jong [and others] -- Gene cloning and expression profiling by rapid amplification of gene inserts with universal vector primers / S.H. Huang, H.Y. Wu and A.Y. Jong -- The isolation of DNA sequences flanking TN5 transposon insertions by inverse PCR / V.J. Martin and W.W. Mohn -- Rapid amplification of genomic DNA sequences tagged by insertional mutagenesis / M. Celerin and K.T. Chun -- Isolation of large-terminal sequences of bac inserts based on double-restriction-enzyme digestion followed by anchored PCR / Z.N. Yang and T.E. Mirkov -- A "step down" PCR-based technique for walking into and the subsequent direct-sequence analysis of flanking genomic DNA / Z. Zhang and S.J. Gurr -- Use of PCR in library screening. An overview / J. Zhu -- Cloning of homologous genes by gene-capture PCR / R. Mastrangeli and S. Donini -- Rapid and nonradioactive screening of recombinant libraries by PCR / M.W. King -- Rapid cDNA cloning by PCR screening (RC-PCR) / T. Takumi -- Generation and PCR screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method") / M. Lardelli -- PCR-based screening for bacterial artificial chromosome libraries / Y. Yasukochi -- A 384-well microtiter-plate-based template preparation and sequencing method / L. He and K. Wang -- A microtiter-plate-based high throughput PCR product purification method / R. Smith and K. Wang
Dimensions
unknown
Edition
2nd ed.
Extent
1 online resource (xiv, 439 pages)
Form of item
online
Isbn
9780585403342
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)50175114
  • (OCoLC)ocm50175114
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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