The Resource Molecular methods in developmental biology : Xenopus and zebrafish, edited by Matthew Guille, (electronic resource)

Molecular methods in developmental biology : Xenopus and zebrafish, edited by Matthew Guille, (electronic resource)

Label
Molecular methods in developmental biology : Xenopus and zebrafish
Title
Molecular methods in developmental biology
Title remainder
Xenopus and zebrafish
Statement of responsibility
edited by Matthew Guille
Contributor
Subject
Genre
Language
eng
Summary
In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development
Member of
Cataloging source
N$T
Dewey number
571.8/616
Illustrations
illustrations
Index
index present
LC call number
QL959
LC item number
.M668 1999eb
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 127
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1
  • QH 506
NLM item number
  • ME9616J v.127 1999
  • M719253 1999
http://library.link/vocab/relatedWorkOrContributorName
Guille, Matthew
Series statement
Methods in molecular biology
Series volume
v. 127
http://library.link/vocab/subjectName
  • Vertebrates
  • Molecular biology
  • Xenopus
  • Zebra danio
  • Developmental biology
  • Cells
  • Zebra danio
  • Embryology
  • Vertebrates
  • Molecular Biology
  • Xenopus
  • Zebrafish
  • Xenopus iaevis
  • SCIENCE
  • Wetenschappelijke technieken
Label
Molecular methods in developmental biology : Xenopus and zebrafish, edited by Matthew Guille, (electronic resource)
Link
http://dx.doi.org/10.1385/1592596789
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The animal cap assay / Jeremy Green -- Cell and tissue transplantation in zebrafish embryos / Toshiro Mizuno, Minori Shinya, and Hiroyuki Takeda -- Ribonuclease protection analysis of gene expression in Xenopus / Craig S. Newman and Paul A. Krieg -- Quantitative analysis of mRNA levels in Xenopus embryos by reverse transcriptase-polymerase chain reaction (RT-PCR) / Oliver C. Steinbach and Ralph A.W. Rupp -- Wholemount in situ hybridization of Xenopus and zebrafish embryos / Joanne Broadbent and E. Mary Read -- In situ hybridization to sections of Xenopus embryos / David Bertwistle -- Zebrafish immunohistochemistry / Rachel Macdonald -- Immunohistochemistry of Xenopus embryos / Carl Robinson and Matt Guille -- Preparation and testing of synthetic mRNA for microinjection / Wendy Moore and Matt Guille -- Microinjection into Xenopus oocytes and embryos / Matt Guille -- Microinjection into zebrafish embryos / Qiling Xu -- Expression from DNA injected into Xenopus embryos / Ondine Cleaver and Paul A. Krieg -- Promoter analysis in zebrafish embryos / Jos Joore -- Transient transgenesis in Xenopus laevis facilitated by AAV-ITRs / Yuchang Fu, Donghui Kan, and Sylvia Evans -- Band-shift analysis using crude oocyte and embryo extracts from Xenopus laevis / Rob Orford and Matt Guille -- DNA-footprinting using crude embryonic extracts from Xenopus laevis / Rob Orford, Darren Mernagh, and Matt Guille -- Mapping protein-DNA interactions using in vivo footprinting / David Warshawsky and Leo Miller
Dimensions
unknown
Extent
1 online resource (xii, 217 pages)
File format
unknown
Form of item
online
Isbn
9780896037908
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)170931971
  • (OCoLC)ocn170931971
Label
Molecular methods in developmental biology : Xenopus and zebrafish, edited by Matthew Guille, (electronic resource)
Link
http://dx.doi.org/10.1385/1592596789
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
The animal cap assay / Jeremy Green -- Cell and tissue transplantation in zebrafish embryos / Toshiro Mizuno, Minori Shinya, and Hiroyuki Takeda -- Ribonuclease protection analysis of gene expression in Xenopus / Craig S. Newman and Paul A. Krieg -- Quantitative analysis of mRNA levels in Xenopus embryos by reverse transcriptase-polymerase chain reaction (RT-PCR) / Oliver C. Steinbach and Ralph A.W. Rupp -- Wholemount in situ hybridization of Xenopus and zebrafish embryos / Joanne Broadbent and E. Mary Read -- In situ hybridization to sections of Xenopus embryos / David Bertwistle -- Zebrafish immunohistochemistry / Rachel Macdonald -- Immunohistochemistry of Xenopus embryos / Carl Robinson and Matt Guille -- Preparation and testing of synthetic mRNA for microinjection / Wendy Moore and Matt Guille -- Microinjection into Xenopus oocytes and embryos / Matt Guille -- Microinjection into zebrafish embryos / Qiling Xu -- Expression from DNA injected into Xenopus embryos / Ondine Cleaver and Paul A. Krieg -- Promoter analysis in zebrafish embryos / Jos Joore -- Transient transgenesis in Xenopus laevis facilitated by AAV-ITRs / Yuchang Fu, Donghui Kan, and Sylvia Evans -- Band-shift analysis using crude oocyte and embryo extracts from Xenopus laevis / Rob Orford and Matt Guille -- DNA-footprinting using crude embryonic extracts from Xenopus laevis / Rob Orford, Darren Mernagh, and Matt Guille -- Mapping protein-DNA interactions using in vivo footprinting / David Warshawsky and Leo Miller
Dimensions
unknown
Extent
1 online resource (xii, 217 pages)
File format
unknown
Form of item
online
Isbn
9780896037908
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)170931971
  • (OCoLC)ocn170931971

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