The Resource Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow

Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow

Label
Molecular biology techniques : a classroom laboratory manual
Title
Molecular biology techniques
Title remainder
a classroom laboratory manual
Statement of responsibility
Susan Carson, Heather Miller, D. Scott Witherow
Creator
Contributor
Subject
Genre
Language
eng
Summary
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein-students can actually visualize positive clones following IPTG induction. *Cover basic concepts and techniques used in molecular biology research labs *Student-tested labs proven successful in a real classroom laboratories *Exercises simulate a cloning project that would be performed in a real research lab *"Project" approach to experiments gives students an overview of the entire process *Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Member of
Cataloging source
E7B
http://library.link/vocab/creatorName
Carson, Sue
Illustrations
illustrations
Index
index present
LC call number
QH506
LC item number
.C36 2012eb
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • handbooks
NLM call number
QU 25
http://library.link/vocab/relatedWorkOrContributorName
  • Miller, Heather B
  • Witherow, D. Scott
Series statement
ClinicalKey
http://library.link/vocab/subjectName
  • Molecular biology
  • Molecular biology
Label
Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
Link
https://ezproxy.lib.ou.edu/login?url=https://www.sciencedirect.com/science/book/9780123855442
Instantiates
Publication
Note
Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xxvi, 200 pages)
Form of item
online
Isbn
9780123855459
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
  • ebs 2016
  • Elsevier
Other physical details
color illustrations
Specific material designation
remote
System control number
  • (OCoLC)763161431
  • (OCoLC)ocn763161431
Label
Molecular biology techniques : a classroom laboratory manual, Susan Carson, Heather Miller, D. Scott Witherow
Link
https://ezproxy.lib.ou.edu/login?url=https://www.sciencedirect.com/science/book/9780123855442
Publication
Note
Rev. of: Manipulation and expression of recombinant DNA / Susan Carson, Dominique Robertson. 2nd ed. c2006
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions
Dimensions
unknown
Edition
3rd ed.
Extent
1 online resource (xxvi, 200 pages)
Form of item
online
Isbn
9780123855459
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
  • ebs 2016
  • Elsevier
Other physical details
color illustrations
Specific material designation
remote
System control number
  • (OCoLC)763161431
  • (OCoLC)ocn763161431

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