The Resource Microbial Gene Essentiality: Protocols and Bioinformatics, edited by Andrei L. Osterman, Svetlana Y. Gerdes, (electronic resource)

Microbial Gene Essentiality: Protocols and Bioinformatics, edited by Andrei L. Osterman, Svetlana Y. Gerdes, (electronic resource)

Label
Microbial Gene Essentiality: Protocols and Bioinformatics
Title
Microbial Gene Essentiality: Protocols and Bioinformatics
Statement of responsibility
edited by Andrei L. Osterman, Svetlana Y. Gerdes
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Microbial Gene Essentiality: Protocols and Bioinformatics contains a comprehensive collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies, developed and presented by the leading groups in the field. In addition to wet-lab protocols, the book describes (i) statistical methods essential for planning successful large-scale essentiality screens, as well for data evaluation and analysis; (ii) in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and (iii) data integration and comparative analysis in the context of genomic databases. This volume provides researchers with a first-stop guide for choosing the most appropriate strategy for planned essentiality studies. Experimental and computational aspects are equally important in genome-scale gene essentiality analysis, as in all other genomic technologies, and Microbial Gene Essentiality: Protocols and Bioinformatics reflects both of these aspects. All protocols follow the successful Methods in Molecular BiologyTM series format, each offering an introduction outlining the principles behind the techniques, step-by-step instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls, and are intended for both novice and expert scientists
Member of
Dewey number
579/.135
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • TETrNBd4vyc
  • xLpLuujpEaM
Image bit depth
0
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Osterman, Andrei L.
  • Gerdes, Svetlana Y.
Series statement
Methods in Molecular Biology,
Series volume
416
http://library.link/vocab/subjectName
  • Biochemistry
  • Cytology
  • Human genetics
  • Biochemistry, general
  • Cell Biology
  • Human Genetics
Label
Microbial Gene Essentiality: Protocols and Bioinformatics, edited by Andrei L. Osterman, Svetlana Y. Gerdes, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Overview of Whole-Genome Essentiality Analysis -- Experimental Protocols -- Transposon-Based Strategies for the Identification of Essential Bacterial Genes -- Identification and Analysis of Essential Genes in Haemophilus influenzae -- Transposon Site Hybridization in Mycobacterium tuberculosis -- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis -- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting -- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis -- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast -- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1 -- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality -- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12 -- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12 -- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12 -- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains -- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray -- Scarless Engineering of the Escherichia coli Genome -- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System -- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome -- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library -- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus -- of Conditional Lethal Amber Mutations in Escherichia coli -- Bioinformatics -- Statistical Methods for Building Random Transposon Mutagenesis Libraries -- Statistical Evaluation of Genetic Footprinting Data -- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results? -- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays -- Profiling of Escherichia coli Chromosome Database -- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes -- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis -- Design and Application of Genome-Scale Reconstructed Metabolic Models -- Predicting Gene Essentiality Using Genome-Scale in Silico Models -- Comparative Approach to Analysis of Gene Essentiality
Dimensions
unknown
Edition
1st ed. 2008.
Extent
1 online resource (XIV, 486 p.)
File format
multiple file formats
Form of item
online
Isbn
9781597453219
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-321-9
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000393132
  • (SSID)ssj0000324826
  • (PQKBManifestationID)11282676
  • (PQKBTitleCode)TC0000324826
  • (PQKBWorkID)10320154
  • (PQKB)10204335
  • (DE-He213)978-1-59745-321-9
  • (EXLCZ)991000000000393132
Label
Microbial Gene Essentiality: Protocols and Bioinformatics, edited by Andrei L. Osterman, Svetlana Y. Gerdes, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Overview of Whole-Genome Essentiality Analysis -- Experimental Protocols -- Transposon-Based Strategies for the Identification of Essential Bacterial Genes -- Identification and Analysis of Essential Genes in Haemophilus influenzae -- Transposon Site Hybridization in Mycobacterium tuberculosis -- Essential Genes in the Infection Model of Pseudomonas aeruginosa PCR-Based Signature-Tagged Mutagenesis -- Whole-Genome Detection of Conditionally Essential and Dispensable Genes in Escherichia coli via Genetic Footprinting -- Generating a Collection of Insertion Mutations in the Staphylococcus aureus Genome Using bursa aurealis -- Multipurpose Transposon Insertion Libraries for Large-Scale Analysis of Gene Function in Yeast -- How to Make a Defined Near-Saturation Mutant Library. Case 1: Pseudomonas aeruginosa PAO1 -- Comparing Insertion Libraries in Two Pseudomonas aeruginosa Strains to Assess Gene Essentiality -- The Construction of Systematic In-Frame, Single-Gene Knockout Mutant Collection in Escherichia coli K-12 -- The Applications of Systematic In-Frame, Single-Gene Knockout Mutant Collection of Escherichia coli K-12 -- A Novel, Simple, High-Throughput Method for Isolation of Genome-Wide Transposon Insertion Mutants of Escherichia coli K-12 -- High-Throughput Creation of a Whole-Genome Collection of Yeast Knockout Strains -- Analysis of Genetic Interactions on a Genome-Wide Scale in Budding Yeast: Diploid-Based Synthetic Lethality Analysis by Microarray -- Scarless Engineering of the Escherichia coli Genome -- Minimization of the Escherichia coli Genome Using the Tn5-Targeted Cre/loxP Excision System -- Construction of Long Chromosomal Deletion Mutants of Escherichia coli and Minimization of the Genome -- Identification of Essential Genes in Staphylococcus aureus by Construction and Screening of Conditional Mutant Library -- Techniques for the Isolation and Use of Conditionally Expressed Antisense RNA to Achieve Essential Gene Knockdowns in Staphylococcus aureus -- of Conditional Lethal Amber Mutations in Escherichia coli -- Bioinformatics -- Statistical Methods for Building Random Transposon Mutagenesis Libraries -- Statistical Evaluation of Genetic Footprinting Data -- Modeling Competitive Outgrowth of Mutant Populations: Why Do Essentiality Screens Yield Divergent Results? -- Statistical Analysis of Fitness Data Determined by TAG Hybridization on Microarrays -- Profiling of Escherichia coli Chromosome Database -- Gene Essentiality Analysis Based on DEG, a Database of Essential Genes -- Detection of Essential Genes in Streptococcus pneumoniae Using Bioinformatics and Allelic Replacement Mutagenesis -- Design and Application of Genome-Scale Reconstructed Metabolic Models -- Predicting Gene Essentiality Using Genome-Scale in Silico Models -- Comparative Approach to Analysis of Gene Essentiality
Dimensions
unknown
Edition
1st ed. 2008.
Extent
1 online resource (XIV, 486 p.)
File format
multiple file formats
Form of item
online
Isbn
9781597453219
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-59745-321-9
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000393132
  • (SSID)ssj0000324826
  • (PQKBManifestationID)11282676
  • (PQKBTitleCode)TC0000324826
  • (PQKBWorkID)10320154
  • (PQKB)10204335
  • (DE-He213)978-1-59745-321-9
  • (EXLCZ)991000000000393132

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