The Resource Laboratory techniques in biochemistry and molecular biology, Volume 14, A guidebook to lipoprotein technique, G.L. Mills and Patricia A. Lane and P.K. Weech, (electronic resource)

Laboratory techniques in biochemistry and molecular biology, Volume 14, A guidebook to lipoprotein technique, G.L. Mills and Patricia A. Lane and P.K. Weech, (electronic resource)

Label
Laboratory techniques in biochemistry and molecular biology, Volume 14, A guidebook to lipoprotein technique
Title
Laboratory techniques in biochemistry and molecular biology
Title number
Volume 14
Title part
A guidebook to lipoprotein technique
Statement of responsibility
G.L. Mills and Patricia A. Lane and P.K. Weech
Creator
Contributor
Subject
Genre
Language
  • eng
  • eng
Summary
This volume is a comprehensive collection of methods for the isolation, characterization, analysis and estimation of soluble lipoproteins. It surveys each aspect of lipoprotein technology in a critical manner which will enable the investigator to select the methods most relevant to his requirements. Having made his choice, he will then find a detailed description of how to perform the technique
Member of
Is Subseries of
Cataloging source
MiAaPQ
http://library.link/vocab/creatorName
Mills, G. L
Dewey number
  • 574.19/2/028 s 612/.12
  • 574.192028 s612.12
Illustrations
illustrations
Index
no index present
Language note
English
LC call number
QP519.7
LC item number
.L3314 1984
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Lane, Patricia A
  • Weech, P. K
Series statement
Laboratory techniques in biochemistry and molecular biology
Series volume
14
http://library.link/vocab/subjectName
  • Biochemistry
  • Molecular biology
Label
Laboratory techniques in biochemistry and molecular biology, Volume 14, A guidebook to lipoprotein technique, G.L. Mills and Patricia A. Lane and P.K. Weech, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; A Guidebook to Lipoprotein Technique; Copyright Page; Contents; Preface; Glossary of lipoprotein nomenclature as used in this manual; Glossary of apo-lipoprotein nomenclature as used in this manual; Chapter 1. Brief introduction to the plasma lipoproteins; 1.1. Nomenclature of the lipoproteins; 1.2. Nomenclature of apo-lipoproteins; Chapter 2. The isolation and purification of plasma lipoproteins; 2.1. Isolation by ultracentrifugation; 2.2. Isolation by precipitation; 2.3. Isolation by chromatography; 2.4. Isolation by preparative electrophoresis
  • 2.5. The isolation of Lp(a) and LP-X2.6. The preservation of isolated lipoproteins; Chapter 3. The characterisation of plasma lipoproteins: intro: duction to principles and methods; 3.1. The purity of lipoprotein preparations; 3.2. Physical and chemical characterisation; 3.3. Heterogeneity and polydispersity; Chapter 4. Physical characterisation of lipoproteins; 4.1. The analytical ultracentrifuge; 4.2. Partial specific volume (hydrated density); 4.3. The analysis of polydispersity in the preparative ultracentrifuge; 4.4. Gel filtration; 4.5. Electron microscopy
  • Chapter 5. Electrophoretic analysis of intact lipoproteins5.1. Preparation of samples; 5.2. Electrophoresis on paper; 5.3. Electrophoresis on cellulose acetate membranes; 5.4. Electrophoresis in agarose gel; 5.5. Electrophoresis in polyacrylamide gel; 5.6. Immunoelectrophoresis; 5.7. Two-dimensial immuno-electrophoresis; 5.8. Isoelectric focussing; Chapter 6. The chemical characterisation of lipoproteins; 6.1. Estimation of total lipoprotein; 6.2. Separation of the lipid and protein moieties; 6.3. Analysis of the lipid moiety; 6.4. Analysis of the protein moiety
  • 6.5. Qualitative analysis of apo-lipoproteins by immunodiffusion6.6. Identification and estimation of carbohydrates; 6.7. Quantitative analysis of lipoproteins by micro-scale thin-layer chromatography; Chapter 7. The estimation of lipoproteins; 7.1. Estimation of lipoproteins by refractometry; 7.2. Nephelometry; 7.3. The estimation of lipoproteins by chemical methods; 7.4. Estimation of LP-X; 7.5. Immunoassay of lipoproteins; Chapter 8. Isolation and assay of apo-lipoproteins; 8.1. Isolation of apo-lipoprotein A-1; 8.2. Isolation of apo-lipoprotein A-II; 8.3. Isolation of apo-lipoprotein A-IV
  • 8.4. Isolation of apo-lipoprotein B8.5. Isolation of the C apo-lipoproteins; 8.6. Isolation of apo-lipoprotein D; 8.7. Isolation of apo-lipoprotein E; 8.8. Isolation of apo-lipoprotein F; 8.9. The estimation of apo-lipoproteins; Appendix 1. The collection and preservation of blood plasma; Appendix 2. Methods of concentrating lipoprotein solutions; Appendix 3. The extraction of lipid from lipoproteins; Appendix 4. The preparation of stains
Dimensions
unknown
Extent
1 online resource (527 p.)
Form of item
online
Isbn
9780080858838
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (CKB)1000000000555035
  • (EBL)402689
  • (OCoLC)476211866
  • (SSID)ssj0000166509
  • (PQKBManifestationID)11924565
  • (PQKBTitleCode)TC0000166509
  • (PQKBWorkID)10160825
  • (PQKB)10944619
  • (MiAaPQ)EBC402689
  • (EXLCZ)991000000000555035
Label
Laboratory techniques in biochemistry and molecular biology, Volume 14, A guidebook to lipoprotein technique, G.L. Mills and Patricia A. Lane and P.K. Weech, (electronic resource)
Publication
Note
Description based upon print version of record
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
  • Front Cover; A Guidebook to Lipoprotein Technique; Copyright Page; Contents; Preface; Glossary of lipoprotein nomenclature as used in this manual; Glossary of apo-lipoprotein nomenclature as used in this manual; Chapter 1. Brief introduction to the plasma lipoproteins; 1.1. Nomenclature of the lipoproteins; 1.2. Nomenclature of apo-lipoproteins; Chapter 2. The isolation and purification of plasma lipoproteins; 2.1. Isolation by ultracentrifugation; 2.2. Isolation by precipitation; 2.3. Isolation by chromatography; 2.4. Isolation by preparative electrophoresis
  • 2.5. The isolation of Lp(a) and LP-X2.6. The preservation of isolated lipoproteins; Chapter 3. The characterisation of plasma lipoproteins: intro: duction to principles and methods; 3.1. The purity of lipoprotein preparations; 3.2. Physical and chemical characterisation; 3.3. Heterogeneity and polydispersity; Chapter 4. Physical characterisation of lipoproteins; 4.1. The analytical ultracentrifuge; 4.2. Partial specific volume (hydrated density); 4.3. The analysis of polydispersity in the preparative ultracentrifuge; 4.4. Gel filtration; 4.5. Electron microscopy
  • Chapter 5. Electrophoretic analysis of intact lipoproteins5.1. Preparation of samples; 5.2. Electrophoresis on paper; 5.3. Electrophoresis on cellulose acetate membranes; 5.4. Electrophoresis in agarose gel; 5.5. Electrophoresis in polyacrylamide gel; 5.6. Immunoelectrophoresis; 5.7. Two-dimensial immuno-electrophoresis; 5.8. Isoelectric focussing; Chapter 6. The chemical characterisation of lipoproteins; 6.1. Estimation of total lipoprotein; 6.2. Separation of the lipid and protein moieties; 6.3. Analysis of the lipid moiety; 6.4. Analysis of the protein moiety
  • 6.5. Qualitative analysis of apo-lipoproteins by immunodiffusion6.6. Identification and estimation of carbohydrates; 6.7. Quantitative analysis of lipoproteins by micro-scale thin-layer chromatography; Chapter 7. The estimation of lipoproteins; 7.1. Estimation of lipoproteins by refractometry; 7.2. Nephelometry; 7.3. The estimation of lipoproteins by chemical methods; 7.4. Estimation of LP-X; 7.5. Immunoassay of lipoproteins; Chapter 8. Isolation and assay of apo-lipoproteins; 8.1. Isolation of apo-lipoprotein A-1; 8.2. Isolation of apo-lipoprotein A-II; 8.3. Isolation of apo-lipoprotein A-IV
  • 8.4. Isolation of apo-lipoprotein B8.5. Isolation of the C apo-lipoproteins; 8.6. Isolation of apo-lipoprotein D; 8.7. Isolation of apo-lipoprotein E; 8.8. Isolation of apo-lipoprotein F; 8.9. The estimation of apo-lipoproteins; Appendix 1. The collection and preservation of blood plasma; Appendix 2. Methods of concentrating lipoprotein solutions; Appendix 3. The extraction of lipid from lipoproteins; Appendix 4. The preparation of stains
Dimensions
unknown
Extent
1 online resource (527 p.)
Form of item
online
Isbn
9780080858838
Media category
computer
Media type code
  • c
Specific material designation
remote
System control number
  • (CKB)1000000000555035
  • (EBL)402689
  • (OCoLC)476211866
  • (SSID)ssj0000166509
  • (PQKBManifestationID)11924565
  • (PQKBTitleCode)TC0000166509
  • (PQKBWorkID)10160825
  • (PQKB)10944619
  • (MiAaPQ)EBC402689
  • (EXLCZ)991000000000555035

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