The Resource Homing endonucleases : methods and protocols, edited by David R. Edgell

Homing endonucleases : methods and protocols, edited by David R. Edgell

Label
Homing endonucleases : methods and protocols
Title
Homing endonucleases
Title remainder
methods and protocols
Statement of responsibility
edited by David R. Edgell
Contributor
Editor
Subject
Genre
Language
eng
Summary
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases
Member of
Cataloging source
GW5XE
Dewey number
572.8
Illustrations
illustrations
Index
index present
LC call number
QP609.E44
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
Edgell, David R.
Series statement
Methods in Molecular Biology,
Series volume
1123
http://library.link/vocab/subjectName
  • Biomedicine
  • Human Genetics
  • Genetic Engineering
  • Endonucleases
  • Endonucleases
Label
Homing endonucleases : methods and protocols, edited by David R. Edgell
Link
Instantiates
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Mapping free-standing homing endonuclease promoters using 5'RLM-RACE
  • Ewan A. Gibb
  • PCR analysis of chloroplast double-strand break (DSB) repair products induced by I-CreII in chlamydomonas and arabidopsis
  • Taegun Kwon [and three others ]
  • A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases
  • Ning Sun and Huimin Zhao
  • Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection
  • Jason M. Wolfs, Benjamin P. Kleinstiver, and David R. Edgell
  • A yeast-based recombination assay for homing endonuclease activity
  • Jean-Charles Epinat
  • Homing endonucleases : from genetic anomalies to programmable genomic clippers
  • Rapid determination of homing endonuclease DNA binding specificity profile
  • Lei Zhao and Barry L. Stoddard
  • Quantifying the information content of homing endonuclease target sites by single base pair profiling
  • Joshua I. Friedman, Hui Li, and Raymond J. Monnat, Jr.
  • Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries/
  • Hui Li and Raymond J. Monnat Jr.
  • Homing endonuclease target determination Using SELEX adapted for yeast surface display
  • Kyle Jacoby and Andrew M. Scharenberg
  • Engineering and flow-cytometric analysis of chimeric LAGLIDADG homing endonucleases from homologous I-OnuI-family enzymes
  • Sarah K. Baxter, Andrew M. Scharenberg, and Abigail R. Lambert
  • Marlene Belfort and Richard P. Bonocora
  • Bioinformatics identification of coevolving residues
  • Russell J. Dickson and Gregory B. Gloor
  • Identification and analysis of genomic homing endonuclease target sites
  • Stefan Pellenz and Raymond J. Monnat Jr.
  • Redesigning the specificity of protein-DNA interactions with Rosetta
  • Summer Thyme and David Baker
  • Bioinformatic identification of homing endonucleases and their target sites
  • Eyal Privman
  • PCR-based bioprospecting for homing endonucleases in fungal mitochondrial rRNA genes
  • Mohamed Hafez [and four others.]
  • Mapping homing endonuclease cleavage sites using in vitro generated protein
  • Richard P. Bonocora and Marlene Belfort
Dimensions
unknown
Extent
1 online resource (x, 284 pages)
File format
unknown
Form of item
online
Isbn
9781627039680
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-968-0
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)878058669
  • (OCoLC)ocn878058669
Label
Homing endonucleases : methods and protocols, edited by David R. Edgell
Link
Publication
Copyright
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Mapping free-standing homing endonuclease promoters using 5'RLM-RACE
  • Ewan A. Gibb
  • PCR analysis of chloroplast double-strand break (DSB) repair products induced by I-CreII in chlamydomonas and arabidopsis
  • Taegun Kwon [and three others ]
  • A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases
  • Ning Sun and Huimin Zhao
  • Rapid screening of endonuclease target site preference using a modified bacterial two-plasmid selection
  • Jason M. Wolfs, Benjamin P. Kleinstiver, and David R. Edgell
  • A yeast-based recombination assay for homing endonuclease activity
  • Jean-Charles Epinat
  • Homing endonucleases : from genetic anomalies to programmable genomic clippers
  • Rapid determination of homing endonuclease DNA binding specificity profile
  • Lei Zhao and Barry L. Stoddard
  • Quantifying the information content of homing endonuclease target sites by single base pair profiling
  • Joshua I. Friedman, Hui Li, and Raymond J. Monnat, Jr.
  • Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries/
  • Hui Li and Raymond J. Monnat Jr.
  • Homing endonuclease target determination Using SELEX adapted for yeast surface display
  • Kyle Jacoby and Andrew M. Scharenberg
  • Engineering and flow-cytometric analysis of chimeric LAGLIDADG homing endonucleases from homologous I-OnuI-family enzymes
  • Sarah K. Baxter, Andrew M. Scharenberg, and Abigail R. Lambert
  • Marlene Belfort and Richard P. Bonocora
  • Bioinformatics identification of coevolving residues
  • Russell J. Dickson and Gregory B. Gloor
  • Identification and analysis of genomic homing endonuclease target sites
  • Stefan Pellenz and Raymond J. Monnat Jr.
  • Redesigning the specificity of protein-DNA interactions with Rosetta
  • Summer Thyme and David Baker
  • Bioinformatic identification of homing endonucleases and their target sites
  • Eyal Privman
  • PCR-based bioprospecting for homing endonucleases in fungal mitochondrial rRNA genes
  • Mohamed Hafez [and four others.]
  • Mapping homing endonuclease cleavage sites using in vitro generated protein
  • Richard P. Bonocora and Marlene Belfort
Dimensions
unknown
Extent
1 online resource (x, 284 pages)
File format
unknown
Form of item
online
Isbn
9781627039680
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-62703-968-0
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)878058669
  • (OCoLC)ocn878058669

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