The Resource Hematopoietic stem cell protocols, edited by Christopher A. Klug and Craig T. Jordan, (electronic resource)

Hematopoietic stem cell protocols, edited by Christopher A. Klug and Craig T. Jordan, (electronic resource)

Label
Hematopoietic stem cell protocols
Title
Hematopoietic stem cell protocols
Statement of responsibility
edited by Christopher A. Klug and Craig T. Jordan
Contributor
Subject
Genre
Language
eng
Summary
Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration. In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laboratory methods for investigating this critically important cell type. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Many of the protocols are contributed by those researchers who first developed and introduced the procedures to the scientific community. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and such blood cancers as leukemia
Member of
Cataloging source
COO
Dewey number
611/.41
Illustrations
illustrations
Index
index present
LC call number
QP92
LC item number
.H4534 2002
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
WH 380
NLM item number
H48693 2002
http://library.link/vocab/relatedWorkOrContributorName
  • Klug, Christopher A
  • Jordan, Craig T
Series statement
Methods in molecular medicine
Series volume
63
http://library.link/vocab/subjectName
  • Hematopoietic stem cells
  • Hematopoietic Stem Cells
  • Cytological Techniques
  • Hematopoietic Stem Cells
  • Sistema hematopoyético
  • Cellules souches hématopoïétiques
  • SCIENCE
  • MEDICAL
  • Hematopoietic stem cells
  • Stamcellen
  • Bloedvorming
  • Wetenschappelijke technieken
Label
Hematopoietic stem cell protocols, edited by Christopher A. Klug and Craig T. Jordan, (electronic resource)
Link
http://dx.doi.org/10.1385/159259140X
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation and analysis of hematopoietic stem cells from mouse embryos / E. Dzierzak -- The purification of mouse hematopoietic stem cells at sequential stages of maturation / S.J. Morrison -- Flow cytometry and immunoselection of human stem cells / T.E. Thomas -- Method for purification of human hematopoietic stem cells by flow cytometry / M.J. Reitsma -- Noninvasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation / G.G. Bradford -- Isolation and characterization of primitive hematopoietic cells based on their position in the cell cycle / E.F. Srour -- Hematopoietic colony-forming cells / M. Ogawa -- Long-term culture-initiating cell assays for human and murine cells / C.L. Miller -- The cobblestone-area-forming cell assay / G. Haan -- CFU-S: an assay for pluripotent myelopoietic stem cells / E.A. McCulloch -- Intrathymic injection for analysis of T-cell progenitor activity / L. Jerabek -- Quantitation of murine and human hematopoietic stem cells by limiting dilution analysis in competitively repopulated hosts / S.J. Szilvassy -- Ex vivo expansion of human and murine hematopoietic stem cells / C.L. Miller -- Hematopoietic development of ES cells in culture / G.M. Keller -- Genetic modification of murine hematopoietic stem cells by retroviruses / C.P. Kalberer -- Retroviral transduction of FACS-purified hematopoietic stem cells / C.V. Cotta -- Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells / M.A. Dao -- Production of lentiviral vector supernatants and transduction of cellular targets / R.E. Sutton -- Reverse transcriptase-PCR analysis of gene expressiong in hematopoietic stem cells / D. Orlic -- Identification of differentially expressed genes in sorted cell populations by two-dimensional gene-expression fingerprinting / A.V. Belyavsky
Dimensions
unknown
Extent
1 online resource (xi, 332 pages)
Form of item
online
Isbn
9781592591404
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)81485183
  • (OCoLC)ocm81485183
Label
Hematopoietic stem cell protocols, edited by Christopher A. Klug and Craig T. Jordan, (electronic resource)
Link
http://dx.doi.org/10.1385/159259140X
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Isolation and analysis of hematopoietic stem cells from mouse embryos / E. Dzierzak -- The purification of mouse hematopoietic stem cells at sequential stages of maturation / S.J. Morrison -- Flow cytometry and immunoselection of human stem cells / T.E. Thomas -- Method for purification of human hematopoietic stem cells by flow cytometry / M.J. Reitsma -- Noninvasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation / G.G. Bradford -- Isolation and characterization of primitive hematopoietic cells based on their position in the cell cycle / E.F. Srour -- Hematopoietic colony-forming cells / M. Ogawa -- Long-term culture-initiating cell assays for human and murine cells / C.L. Miller -- The cobblestone-area-forming cell assay / G. Haan -- CFU-S: an assay for pluripotent myelopoietic stem cells / E.A. McCulloch -- Intrathymic injection for analysis of T-cell progenitor activity / L. Jerabek -- Quantitation of murine and human hematopoietic stem cells by limiting dilution analysis in competitively repopulated hosts / S.J. Szilvassy -- Ex vivo expansion of human and murine hematopoietic stem cells / C.L. Miller -- Hematopoietic development of ES cells in culture / G.M. Keller -- Genetic modification of murine hematopoietic stem cells by retroviruses / C.P. Kalberer -- Retroviral transduction of FACS-purified hematopoietic stem cells / C.V. Cotta -- Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells / M.A. Dao -- Production of lentiviral vector supernatants and transduction of cellular targets / R.E. Sutton -- Reverse transcriptase-PCR analysis of gene expressiong in hematopoietic stem cells / D. Orlic -- Identification of differentially expressed genes in sorted cell populations by two-dimensional gene-expression fingerprinting / A.V. Belyavsky
Dimensions
unknown
Extent
1 online resource (xi, 332 pages)
Form of item
online
Isbn
9781592591404
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)81485183
  • (OCoLC)ocm81485183

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