The Resource Handbook of vascular biology techniques, Mark Slevin, Garry McDowell, editors

Handbook of vascular biology techniques, Mark Slevin, Garry McDowell, editors

Label
Handbook of vascular biology techniques
Title
Handbook of vascular biology techniques
Statement of responsibility
Mark Slevin, Garry McDowell, editors
Contributor
Editor
Subject
Genre
Language
eng
Summary
A wide range of research methods for the study of vascular development, from basic laboratory protocols to advanced technologies used in clinical practice, are covered in this work. A range of methodologies such as molecular imaging platforms and signalling analysis, along with tumour models are collated here. Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. Readers will discover basic methodologies used for analysis of endothelial cell growth in vitro, including co-culture models of vessel formation. Authors also explore isolation and purification of cells and methods for analysis of data and visualization of localized vasculature with modern imaging platforms. Both animal models and human disease are covered in this work. Each chapter contains helpful sections on trouble shooting, additional notes and links, supporting the reader to carry out protocols. This book will appeal to students, researchers and medical professionals working in all vascular-linked fields such as cardio- and cerebro-vascular, cancer and dementia
Member of
Cataloging source
N$T
Dewey number
612.1/3
Illustrations
illustrations
Index
index present
LC call number
QM178
LC item number
.H36 2015eb
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
  • handbooks
NLM call number
WG 500
http://library.link/vocab/relatedWorkOrContributorName
  • Slevin, Mark
  • McDowell, Garry
http://library.link/vocab/subjectName
  • Blood-vessels
  • Blood-vessels
  • MEDICAL
  • SCIENCE
  • Blood-vessels
  • Blood-vessels
  • Biomedicine
  • Cancer Research
  • Biological Techniques
  • Laboratory Medicine
  • Blood Vessels
Label
Handbook of vascular biology techniques, Mark Slevin, Garry McDowell, editors
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-94-017-9716-0
Instantiates
Publication
Note
Includes index
Antecedent source
unknown
Bibliography note
Includes bibliographical references at the end of each chapters and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preface -- Section 1 In vitro techniques -- In vitro angiogenesis assay -- endothelial migration, proliferation, and tube formation -- Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis -- Induction of hypoxia in vascular endothelial cell culture -- Evaluating In Vitro Angiogenesis using Live Cell Imaging -- Isolation of Endothelial Progenitor Cells (EPCs) -- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis -- Assessment of vascular function and contractility, ex vivo -- Measurement of AÝ uptake by cerebrovascular smooth muscle cells -- Measurement of Intracellular Ca2+ in Human Endothelial cells -- Section 2 In vivo and ex vivo manipulations -- Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion -- Sponge implant model of inflammatory angiogenesis -- The chick embryo chorioallantoic membrane assay -- Dorsal air sac assay -- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals -- Hypoxia-induced retinal angiogenesis in adult zebrafish -- Angiogenesis in regenerating adult zebrafish tail fin -- Methods for studying developmental angiogenesis in zebrafish -- Isolation and Expansion of Brain Microvascular Endothelial Cells -- Section 3 Imaging and histological analysis -- Adipose angiogenesis -- Assessing tumor angiogenesis in histological samples -- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea -- Computed Tomography Angiography: Fundamental techniques and data interpretation -- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation -- Single-Photon Emission Tomography of the brain in vascular pathology -- Section 4 Miscellaneous novel techniques in vascular biology -- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues -- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo -- Vascular Flow Modelling using Computational Fluid Dynamics -- Reverse Transcription Real-Time PCR Protocol for gene expression analyses -- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis -- Genomic microarray analysis -- Selection of appropriate housekeeping genes for quality control -- Endothelial transcriptomic analysis -- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers -- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification -- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood -- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry
Dimensions
unknown
Extent
1 online resource (xii, 477 pages)
File format
unknown
Form of item
online
Isbn
9789401797160
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
SpringerLink
Other control number
10.1007/978-94-017-9716-0
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)905902642
  • (OCoLC)ocn905902642
Label
Handbook of vascular biology techniques, Mark Slevin, Garry McDowell, editors
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-94-017-9716-0
Publication
Note
Includes index
Antecedent source
unknown
Bibliography note
Includes bibliographical references at the end of each chapters and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Preface -- Section 1 In vitro techniques -- In vitro angiogenesis assay -- endothelial migration, proliferation, and tube formation -- Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis -- Induction of hypoxia in vascular endothelial cell culture -- Evaluating In Vitro Angiogenesis using Live Cell Imaging -- Isolation of Endothelial Progenitor Cells (EPCs) -- Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis -- Assessment of vascular function and contractility, ex vivo -- Measurement of AÝ uptake by cerebrovascular smooth muscle cells -- Measurement of Intracellular Ca2+ in Human Endothelial cells -- Section 2 In vivo and ex vivo manipulations -- Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion -- Sponge implant model of inflammatory angiogenesis -- The chick embryo chorioallantoic membrane assay -- Dorsal air sac assay -- Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals -- Hypoxia-induced retinal angiogenesis in adult zebrafish -- Angiogenesis in regenerating adult zebrafish tail fin -- Methods for studying developmental angiogenesis in zebrafish -- Isolation and Expansion of Brain Microvascular Endothelial Cells -- Section 3 Imaging and histological analysis -- Adipose angiogenesis -- Assessing tumor angiogenesis in histological samples -- Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the embryonic mouse skin and adult mouse cornea -- Computed Tomography Angiography: Fundamental techniques and data interpretation -- Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation -- Single-Photon Emission Tomography of the brain in vascular pathology -- Section 4 Miscellaneous novel techniques in vascular biology -- Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues -- Preparation of liposomes with dual fluorophores to follow real-time content release in vivo -- Vascular Flow Modelling using Computational Fluid Dynamics -- Reverse Transcription Real-Time PCR Protocol for gene expression analyses -- Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis -- Genomic microarray analysis -- Selection of appropriate housekeeping genes for quality control -- Endothelial transcriptomic analysis -- Protocol for Multiplex Amplicon Sequencing using Barcoded Primers -- Flow cytometry Enumeration of Hematopoietic & Progenitor Stem Cells: Identification and Quantification -- A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood -- Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry
Dimensions
unknown
Extent
1 online resource (xii, 477 pages)
File format
unknown
Form of item
online
Isbn
9789401797160
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Note
SpringerLink
Other control number
10.1007/978-94-017-9716-0
Other physical details
illustrations (some color)
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)905902642
  • (OCoLC)ocn905902642

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