The Resource Genetic transformation systems in fungi, Volume 1, Marco A. van den Berg, Karunakaran Maruthachalam, editors

Genetic transformation systems in fungi, Volume 1, Marco A. van den Berg, Karunakaran Maruthachalam, editors

Label
Genetic transformation systems in fungi, Volume 1
Title
Genetic transformation systems in fungi
Title number
Volume 1
Statement of responsibility
Marco A. van den Berg, Karunakaran Maruthachalam, editors
Contributor
Editor
Subject
Genre
Language
eng
Summary
Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalia
Member of
Cataloging source
N$T
Dewey number
579/.135
Illustrations
illustrations
Index
index present
LC call number
QK602
LC item number
.G45 2015eb
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Berg, Marco A. van den
  • Maruthachalam, Karunakaran
Series statement
Fungal Biology,
http://library.link/vocab/subjectName
  • Fungi
  • SCIENCE
  • SCIENCE
  • Fungi
  • Botany & plant sciences
  • Plant pathology & diseases
  • Plant reproduction & propagation
  • Life sciences: general issues
  • Developmental biology
Label
Genetic transformation systems in fungi, Volume 1, Marco A. van den Berg, Karunakaran Maruthachalam, editors
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-3-319-10142-2
Instantiates
Publication
Copyright
Note
Includes index
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Preface; Contents; Contributors; Part I: Introduction; 1: Fungal Transformation: From Protoplasts to Targeted Recombination Systems; 1.1 Introduction; 1.1.1 A Historical Perspective of Transformation of Fungi; 1.2 Early Development of Basic Tools for Transformation; 1.2.1 Protoplasts and Lytic Enzymes; 1.2.2 Osmotic Stabilization and Protoplasts Regeneration; 1.3 Alternative Transformation Procedures That Do Not Require Protoplasts; 1.3.1 Electroporation; 1.3.2 Agrobacterium-Mediated Transformation; 1.3.3 Biolistic Transformation; 1.4 Selective Markers; 1.4.1 Nutritional Markers
  • 1.4.2 Metabolic Fitness of Auxotrophic Host Strains: Growth and Secondary Metabolites Production1.4.3 Positive Selection Resistance Markers; 1.5 Autonomously Replicating Plasmids: Stability Problems of Gene Libraries; 1.6 Homologous and Nonhomologous Recombination: Integration of Exogenous Genes; 1.6.1 Targeted Monocopy Integrations at Specific Loci; 1.6.2 The "Two Markers" Selection Strategy for Gene Disruption; 1.6.3 The Nonhomologous End-Joining Mechanism; 1.6.4 Metabolic Fitness of NHEJ-ƯDeficient Mutants; 1.7 Future Outlook; References
  • 2.3.1.1 Cell Type and Growth Phase2.3.1.2 Growth Conditions; 2.3.1.3 Optimal Enzyme Combination for Lysing the Cell Wall; 2.3.1.4 Stabilizing Protoplasts During and After Removal of the Cell Wall; 2.3.1.5 Incubation Time and Temperature for Lysing the Cell Wall; 2.3.1.6 Evaluation and Storage of Protoplasts; 2.3.1.7 Uptake of DNA/Transformation; 2.3.1.8 Incubation Time and Medium for Protoplast Regeneration; 2.4 Concluding Remarks; References; 3: Trichoderma Transformation Methods; 3.1 General Methods; 3.2 Detailed Procedure Description
  • 3.2.1 Transformation of Trichoderma Mediated by Protoplasts3.2.1.1 Growth Conditions and Protoplasts Formation; 3.2.1.2 Protoplasts Transformation; 3.2.2 Transformation of Trichoderma Mediated by A. tumefaciens; 3.3 Alternative Trichoderma Transformation Procedures; 3.3.1 Protoplast Electroporation (S©Łnchez-Torres et al. 1994); 3.3.2 Biolistic (Lorito et al. 1993); 3.3.3 Shock Waves (Maga©ła-Ort©Ưz et al. 2013); 3.4 Notes; References; 4: Transformation of Mucor circinelloides f. lusitanicus Protoplasts; 4.1 Introduction; 4.2 Materials; 4.3 Methods; 4.3.1 M. circinelloides Cell-Wall Isolation
Dimensions
unknown
Extent
1 online resource (xv, 318 pages)
File format
unknown
Form of item
online
Isbn
9783319101422
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
SpringerLink
Other control number
10.1007/978-3-319-10142-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)894509236
  • (OCoLC)ocn894509236
Label
Genetic transformation systems in fungi, Volume 1, Marco A. van den Berg, Karunakaran Maruthachalam, editors
Link
https://ezproxy.lib.ou.edu/login?url=http://link.springer.com/10.1007/978-3-319-10142-2
Publication
Copyright
Note
Includes index
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
txt
Content type MARC source
rdacontent
Contents
  • Preface; Contents; Contributors; Part I: Introduction; 1: Fungal Transformation: From Protoplasts to Targeted Recombination Systems; 1.1 Introduction; 1.1.1 A Historical Perspective of Transformation of Fungi; 1.2 Early Development of Basic Tools for Transformation; 1.2.1 Protoplasts and Lytic Enzymes; 1.2.2 Osmotic Stabilization and Protoplasts Regeneration; 1.3 Alternative Transformation Procedures That Do Not Require Protoplasts; 1.3.1 Electroporation; 1.3.2 Agrobacterium-Mediated Transformation; 1.3.3 Biolistic Transformation; 1.4 Selective Markers; 1.4.1 Nutritional Markers
  • 1.4.2 Metabolic Fitness of Auxotrophic Host Strains: Growth and Secondary Metabolites Production1.4.3 Positive Selection Resistance Markers; 1.5 Autonomously Replicating Plasmids: Stability Problems of Gene Libraries; 1.6 Homologous and Nonhomologous Recombination: Integration of Exogenous Genes; 1.6.1 Targeted Monocopy Integrations at Specific Loci; 1.6.2 The "Two Markers" Selection Strategy for Gene Disruption; 1.6.3 The Nonhomologous End-Joining Mechanism; 1.6.4 Metabolic Fitness of NHEJ-ƯDeficient Mutants; 1.7 Future Outlook; References
  • 2.3.1.1 Cell Type and Growth Phase2.3.1.2 Growth Conditions; 2.3.1.3 Optimal Enzyme Combination for Lysing the Cell Wall; 2.3.1.4 Stabilizing Protoplasts During and After Removal of the Cell Wall; 2.3.1.5 Incubation Time and Temperature for Lysing the Cell Wall; 2.3.1.6 Evaluation and Storage of Protoplasts; 2.3.1.7 Uptake of DNA/Transformation; 2.3.1.8 Incubation Time and Medium for Protoplast Regeneration; 2.4 Concluding Remarks; References; 3: Trichoderma Transformation Methods; 3.1 General Methods; 3.2 Detailed Procedure Description
  • 3.2.1 Transformation of Trichoderma Mediated by Protoplasts3.2.1.1 Growth Conditions and Protoplasts Formation; 3.2.1.2 Protoplasts Transformation; 3.2.2 Transformation of Trichoderma Mediated by A. tumefaciens; 3.3 Alternative Trichoderma Transformation Procedures; 3.3.1 Protoplast Electroporation (S©Łnchez-Torres et al. 1994); 3.3.2 Biolistic (Lorito et al. 1993); 3.3.3 Shock Waves (Maga©ła-Ort©Ưz et al. 2013); 3.4 Notes; References; 4: Transformation of Mucor circinelloides f. lusitanicus Protoplasts; 4.1 Introduction; 4.2 Materials; 4.3 Methods; 4.3.1 M. circinelloides Cell-Wall Isolation
Dimensions
unknown
Extent
1 online resource (xv, 318 pages)
File format
unknown
Form of item
online
Isbn
9783319101422
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
c
Note
SpringerLink
Other control number
10.1007/978-3-319-10142-2
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)894509236
  • (OCoLC)ocn894509236

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