The Resource Gene Probes : Principles and Protocols, edited by Marilena Aquino do Muro, Ralph Rapley, (electronic resource)

Gene Probes : Principles and Protocols, edited by Marilena Aquino do Muro, Ralph Rapley, (electronic resource)

Label
Gene Probes : Principles and Protocols
Title
Gene Probes
Title remainder
Principles and Protocols
Statement of responsibility
edited by Marilena Aquino do Muro, Ralph Rapley
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today
Member of
Is Subseries of
Dewey number
572.8
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • O3Fv4OCiUdM
  • ceDe51MBd5s
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Aquino do Muro, Marilena.
  • Rapley, Ralph.
Series statement
Methods in Molecular Biology,
Series volume
179
http://library.link/vocab/subjectName
  • Biochemistry
  • Biochemistry, general
Label
Gene Probes : Principles and Protocols, edited by Marilena Aquino do Muro, Ralph Rapley, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Target Format and Hybridization Conditions -- End Labeling Procedures -- Photobiotin Labeling -- Nick Translation -- Random Primed Labeling -- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization -- Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors -- The ?-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites -- Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma -- Detection of Chlamydia trachomatis by DNA Amplification -- Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes -- Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction -- Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes -- Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 -- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) -- Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species -- Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes -- In Situ Hybridization of Burkholderia cepacia Cells -- Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil -- Detection of Single-Copy Genes in DNA from Transgenic Plants -- Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission -- Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food -- Polymerase Chain Reaction for Detection of Listeria monocytogenes
Dimensions
unknown
Edition
1st ed. 2002.
Extent
1 online resource (284 p.)
Form of item
online
Isbn
9786610835959
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592384
Specific material designation
remote
System control number
  • (CKB)1000000000239938
  • (EBL)3036893
  • (SSID)ssj0000161309
  • (PQKBManifestationID)11161307
  • (PQKBTitleCode)TC0000161309
  • (PQKBWorkID)10191010
  • (PQKB)10748885
  • (MiAaPQ)EBC3036893
  • (DE-He213)978-1-59259-238-8
  • (EXLCZ)991000000000239938
Label
Gene Probes : Principles and Protocols, edited by Marilena Aquino do Muro, Ralph Rapley, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Target Format and Hybridization Conditions -- End Labeling Procedures -- Photobiotin Labeling -- Nick Translation -- Random Primed Labeling -- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization -- Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors -- The ?-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites -- Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma -- Detection of Chlamydia trachomatis by DNA Amplification -- Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes -- Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction -- Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes -- Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 -- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) -- Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species -- Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes -- In Situ Hybridization of Burkholderia cepacia Cells -- Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil -- Detection of Single-Copy Genes in DNA from Transgenic Plants -- Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission -- Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food -- Polymerase Chain Reaction for Detection of Listeria monocytogenes
Dimensions
unknown
Edition
1st ed. 2002.
Extent
1 online resource (284 p.)
Form of item
online
Isbn
9786610835959
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592384
Specific material designation
remote
System control number
  • (CKB)1000000000239938
  • (EBL)3036893
  • (SSID)ssj0000161309
  • (PQKBManifestationID)11161307
  • (PQKBTitleCode)TC0000161309
  • (PQKBWorkID)10191010
  • (PQKB)10748885
  • (MiAaPQ)EBC3036893
  • (DE-He213)978-1-59259-238-8
  • (EXLCZ)991000000000239938

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