The Resource Gene Knockout Protocols, edited by Martin J. Tymms, Ismail Kola, (electronic resource)

Gene Knockout Protocols, edited by Martin J. Tymms, Ismail Kola, (electronic resource)

Label
Gene Knockout Protocols
Title
Gene Knockout Protocols
Statement of responsibility
edited by Martin J. Tymms, Ismail Kola
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
As the major task of sequencing the human genome nears completion and the full complement of human genes are catalogued, the task of understanding the normal biological functions of genes and how their alteration leads to diseased states becomes more imperative. In Gene Knockout Protocols, highly skilled investigators with extensive experience in gene targeting and mouse genetics describe their best techniques for the design of targeting constructs and for genetic phenotype analysis. These proven methods contain step-by-step instructions, as well as notes on pitfalls to avoid, and emphasize techniques that are relevant to researchers carrying out gene targeting work. These include embryo transplantation, in vitro embryonic stem cell differentiation, creation of aggregation chimeras, mouse pathology, embryo cryopreservation, and transplantation. Issues such as the use of existing mouse mutation resources and the influence of genetic background and epigenetic effects upon phenotype are also covered. State-of-the-art and highly practical, Gene Knockout Protocols not only constitutes an invaluable source of readily reproducible techniques for those just entering the field of gene targeting, but also a key reference for all genetic researchers today
Member of
Is Subseries of
Dewey number
572.8/77
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • pEdA4e5pDdY
  • GhMRrgwMBck
Language note
English
LC call number
  • RB155-155.8
  • QH431
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Tymms, Martin J.
  • Kola, Ismail.
Series statement
Methods in Molecular Biology,
Series volume
158
http://library.link/vocab/subjectName
  • Human genetics
  • Human Genetics
Label
Gene Knockout Protocols, edited by Martin J. Tymms, Ismail Kola, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Overview -- Isolation and Maintenance of Primate Embryonic Stem Cells -- Gene Targeting in ES Cells -- Manipulating Mouse Embryonic Stem Cells -- Gene Targeting in a Centralized Facility -- The LoxP/CRE System and Genome Modification -- Creation and Use of a Cre Recombinase Transgenic Database -- Choice of Mouse Strains for Gene Targeting -- Isolation, Microinjection, and Transfer of Mouse Blastocysts -- Aggregation Chimeras -- How to Study Pathologic Phenotypes of Knockout Mice -- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models -- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function -- Influence of Genetic Background on Knockout Mouse Phenotypes -- Lineage Allocation During Early Embryogenesis -- Generation of Double-Knockout Embryonic Stem Cells -- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes -- Embryonic Stem Cells in the Study of Hematopoiesis -- Interferon-Inducible ES Cell Expression Systems -- Transgenic Studies in the Mouse -- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping -- Epigenetic Effects on Transgene Expression -- Positional-Candidate Cloning of Genes from Mouse Mutants -- Genetically Engineered Mice -- Embryo Cryopreservation for Transgenic Mouse Lines
Dimensions
unknown
Edition
1st ed. 2001.
Extent
1 online resource (444 p.)
Form of item
online
Isbn
9786610830251
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592201
Specific material designation
remote
System control number
  • (CKB)1000000000239936
  • (EBL)3036925
  • (SSID)ssj0000161307
  • (PQKBManifestationID)11163087
  • (PQKBTitleCode)TC0000161307
  • (PQKBWorkID)10199165
  • (PQKB)11137802
  • (MiAaPQ)EBC3036925
  • (DE-He213)978-1-59259-220-3
  • (EXLCZ)991000000000239936
Label
Gene Knockout Protocols, edited by Martin J. Tymms, Ismail Kola, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
Overview -- Isolation and Maintenance of Primate Embryonic Stem Cells -- Gene Targeting in ES Cells -- Manipulating Mouse Embryonic Stem Cells -- Gene Targeting in a Centralized Facility -- The LoxP/CRE System and Genome Modification -- Creation and Use of a Cre Recombinase Transgenic Database -- Choice of Mouse Strains for Gene Targeting -- Isolation, Microinjection, and Transfer of Mouse Blastocysts -- Aggregation Chimeras -- How to Study Pathologic Phenotypes of Knockout Mice -- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models -- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function -- Influence of Genetic Background on Knockout Mouse Phenotypes -- Lineage Allocation During Early Embryogenesis -- Generation of Double-Knockout Embryonic Stem Cells -- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes -- Embryonic Stem Cells in the Study of Hematopoiesis -- Interferon-Inducible ES Cell Expression Systems -- Transgenic Studies in the Mouse -- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping -- Epigenetic Effects on Transgene Expression -- Positional-Candidate Cloning of Genes from Mouse Mutants -- Genetically Engineered Mice -- Embryo Cryopreservation for Transgenic Mouse Lines
Dimensions
unknown
Edition
1st ed. 2001.
Extent
1 online resource (444 p.)
Form of item
online
Isbn
9786610830251
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592201
Specific material designation
remote
System control number
  • (CKB)1000000000239936
  • (EBL)3036925
  • (SSID)ssj0000161307
  • (PQKBManifestationID)11163087
  • (PQKBTitleCode)TC0000161307
  • (PQKBWorkID)10199165
  • (PQKB)11137802
  • (MiAaPQ)EBC3036925
  • (DE-He213)978-1-59259-220-3
  • (EXLCZ)991000000000239936

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