The Resource Electron Microscopy Methods and Protocols, edited by M. A. Nasser Hajibagheri, (electronic resource)

Electron Microscopy Methods and Protocols, edited by M. A. Nasser Hajibagheri, (electronic resource)

Label
Electron Microscopy Methods and Protocols
Title
Electron Microscopy Methods and Protocols
Statement of responsibility
edited by M. A. Nasser Hajibagheri
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual
Member of
Is Subseries of
Dewey number
570/.28/25
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
FwlxvcKjn-U
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Hajibagheri, M. A. Nasser.
Series statement
Methods in Molecular Biology,
Series volume
117
http://library.link/vocab/subjectName
  • Biochemistry
  • Biochemistry, general
Label
Electron Microscopy Methods and Protocols, edited by M. A. Nasser Hajibagheri, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
General Preparation of Material and Staining of Sections -- Negative Staining of Thinly Spread Biological Particulates -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy -- The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling -- The Application of LR Gold Resin for Immunogold Labeling -- Low-Temperature Embedding in Acrylic Resins -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol -- Electron Microscopic Enzyme Cytochemistry -- In Situ Molecular Hybridization Techniques for Ultrathin Sections -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy -- Applications of Electron Microscopy for Studying Protein-DNA Complexes -- X-Ray Microanalysis Techniques
Dimensions
unknown
Edition
1st ed. 1999.
Extent
1 online resource (301 p.)
Form of item
online
Isbn
9781280821592
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592015
Specific material designation
remote
System control number
  • (CKB)111004365738498
  • (EBL)3036942
  • (SSID)ssj0000145153
  • (PQKBManifestationID)11162093
  • (PQKBTitleCode)TC0000145153
  • (PQKBWorkID)10156845
  • (PQKB)11729130
  • (MiAaPQ)EBC3036942
  • (DE-He213)978-1-59259-201-2
  • (EXLCZ)99111004365738498
Label
Electron Microscopy Methods and Protocols, edited by M. A. Nasser Hajibagheri, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Content category
text
Content type code
  • txt
Contents
General Preparation of Material and Staining of Sections -- Negative Staining of Thinly Spread Biological Particulates -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy -- The Production of Cryosections Through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabeling -- The Application of LR Gold Resin for Immunogold Labeling -- Low-Temperature Embedding in Acrylic Resins -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol -- Electron Microscopic Enzyme Cytochemistry -- In Situ Molecular Hybridization Techniques for Ultrathin Sections -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy -- Applications of Electron Microscopy for Studying Protein-DNA Complexes -- X-Ray Microanalysis Techniques
Dimensions
unknown
Edition
1st ed. 1999.
Extent
1 online resource (301 p.)
Form of item
online
Isbn
9781280821592
Media category
computer
Media type code
  • c
Other control number
10.1385/1592592015
Specific material designation
remote
System control number
  • (CKB)111004365738498
  • (EBL)3036942
  • (SSID)ssj0000145153
  • (PQKBManifestationID)11162093
  • (PQKBTitleCode)TC0000145153
  • (PQKBWorkID)10156845
  • (PQKB)11729130
  • (MiAaPQ)EBC3036942
  • (DE-He213)978-1-59259-201-2
  • (EXLCZ)99111004365738498

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