The Resource Developmental biology protocols, Volume II, edited by Rocky S. Tuan and Cecilia W. Lo, (electronic resource)

Developmental biology protocols, Volume II, edited by Rocky S. Tuan and Cecilia W. Lo, (electronic resource)

Label
Developmental biology protocols, Volume II
Title
Developmental biology protocols
Title number
Volume II
Statement of responsibility
edited by Rocky S. Tuan and Cecilia W. Lo
Contributor
Subject
Genre
Language
eng
Summary
The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 2nd volume of three describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. The 1st and 3rd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis
Member of
Cataloging source
N$T
Dewey number
571.8
Illustrations
  • illustrations
  • plates
Index
index present
LC call number
QH506
LC item number
.M45eb vol. 2
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Tuan, Rocky S
  • Lo, Cecilia W
Series statement
Methods in molecular biology
Series volume
v. 136
http://library.link/vocab/subjectName
  • Developmental biology
  • Molecular biology
  • Developmental Biology
  • Molecular Biology
  • SCIENCE
  • Developmental biology
  • Molecular biology
  • Developmental biology -- Laboratory manuals
  • Molecular biology -- Laboratory manuals
Label
Developmental biology protocols, Volume II, edited by Rocky S. Tuan and Cecilia W. Lo, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590659
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Drosophila as a genetic tool to define vertebrate pathway players / N.M. Bonini -- Bioassays for studying the role of the peptide growth factor activin in early amphibian embryogenesis / M. Asashima [and others] -- Analysis of mammary gland morphogenesis / C.D. Roskelley, C. Wu and A.M. Somasiri -- Specification of cardiac mesenchyme and heart morphogenesis in vitro / H.J. Yost -- Craniofacial development and patterning / H. Slavkin, G. Nuckolls and L. Shum -- Craniofacial skeletal morphogenesis in vitro / R.C. Ogle -- Skeletal morphogenesis / S. Mundlos -- Transplantation and culture techniques for the analysis of urodele limb regeneration / D.L. Stocum -- Retroviral infection of t-cell precursors in thymic organ culture / L.M. Spain, L.L. Lau and Y. Takahama -- Assay for the isolation of hepatogenic factors. Key molecules in hepatocyte formation and liver morphogenesis / J.A. Spijkers, T.B. Hakvoort and W.H. Lamers -- Skin morphogenesis. Embryonic chicken skin explant cultures / C.M. Chuong -- Apoptosis in development / L. Lin and Z.F. Zakeri -- Methods to detect patterns of cell death in drosophila / N.M. Bonini -- Mechanisms of neurulation / G.C. Schoenwolf and J.L. Smith -- Neurulation and neural tube closure defects / A. Copp [and others] -- Neural tube defects / T. Inagaki [and others] -- Experimental manipulation and morphometric analysis of neural tube development / M.E. Desmond and P.A. Haas -- Isolation of neuroepithelium and formation of minispheres / M.E. Desmond and M.C. Field -- Examination of normal and abnormal placentation in the mouse / M.R. Blackburn -- Palatal dysmorphogenesis. Palate organ culture / B.D. Abbott -- Palatal dysmorphogenesis. Quantitative rt-pcr / G.A. Held and B.D. Abbott -- Transspecies grafting as a tool to understand the basis of murine developmental limb abnormalities / S.M. Bell, C.M. Schreiner and W.J. Scott -- Assessment of laterality defects in rodent embryos / M. Fujinaga -- Cardiac morphogenesis and dysmorphogenesis. I. Normal development / A. Wessels and R. Markwald -- Analysis of two aspects of left-right patterning of the vertebrate heart. Heart tube position and heart tube chirality / A.J. Chin -- Biologically based risk assessment models for developmental toxicity / C. Lau and R.W. Setzer -- Positional cloning / L. Xu and Y. Li -- Gene trapping in embryonic stem cells in vitro to identify novel developmentally regulated genes in the mouse / G.E. Lyons [and others] -- PCR-based cloning of cortically localized rnas from xenopus oocytes / J. Zhang and M.L. King -- Analysis of mRNA expression during preimplantation development / K.E. Latham, E. De la Casa and R.M. Schultz -- Differential screens with subtracted pcr-generated cDNA libraries from subregions of single mouse embryos / A. Shimono and R.R. Behringer -- HPLC-based mRNA differential display / T.B. Knudsen -- Production of transgenic drosophila / M. Fujioka [and others] -- Transgenic manipulation of the sea urchin embryo / J.P. Rast -- Transgenic zebrafish / S. Lin -- Production of avian chimeras and germline transmission / K.L. Woods [and others] -- Incorporation of genetically modified cells in chicken chimeras / L.D. Zajchowski [and others] -- Long-term culture of chicken blastodermal cells (cbcs) and selection of transfected cbcs using antibiotic resistance / Q. Wei, K.L. Woods and R.J. Etches -- Nuclear transplantation and cloning in mammals / K.E. Latham and M.E. Westhusin -- Production of transgenic mice / J. Richa -- Production of transgenic mice with yeast artificial chromosomes / A. Jakobovits, B.T. Lamb and K.R. Peterson -- Gene-targeting strategies / S.S. Cheah and R.R. Behringer -- Chimeric animals and germline transmission / J.S. Khillan -- Conditional gene knockout using cre recombinase / Y. Le and B. Sauer -- Application of cre/loxp in drosophila. Site-specific recombination and transgene coplacement / M.L. Siegal and D.L. Hartl
Dimensions
unknown
Extent
1 online resource (xxv, 530 pages, [8] pages of plates)
File format
unknown
Form of item
online
Isbn
9781592590650
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592590659
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)170934659
  • (OCoLC)ocn170934659
Label
Developmental biology protocols, Volume II, edited by Rocky S. Tuan and Cecilia W. Lo, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590659
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Drosophila as a genetic tool to define vertebrate pathway players / N.M. Bonini -- Bioassays for studying the role of the peptide growth factor activin in early amphibian embryogenesis / M. Asashima [and others] -- Analysis of mammary gland morphogenesis / C.D. Roskelley, C. Wu and A.M. Somasiri -- Specification of cardiac mesenchyme and heart morphogenesis in vitro / H.J. Yost -- Craniofacial development and patterning / H. Slavkin, G. Nuckolls and L. Shum -- Craniofacial skeletal morphogenesis in vitro / R.C. Ogle -- Skeletal morphogenesis / S. Mundlos -- Transplantation and culture techniques for the analysis of urodele limb regeneration / D.L. Stocum -- Retroviral infection of t-cell precursors in thymic organ culture / L.M. Spain, L.L. Lau and Y. Takahama -- Assay for the isolation of hepatogenic factors. Key molecules in hepatocyte formation and liver morphogenesis / J.A. Spijkers, T.B. Hakvoort and W.H. Lamers -- Skin morphogenesis. Embryonic chicken skin explant cultures / C.M. Chuong -- Apoptosis in development / L. Lin and Z.F. Zakeri -- Methods to detect patterns of cell death in drosophila / N.M. Bonini -- Mechanisms of neurulation / G.C. Schoenwolf and J.L. Smith -- Neurulation and neural tube closure defects / A. Copp [and others] -- Neural tube defects / T. Inagaki [and others] -- Experimental manipulation and morphometric analysis of neural tube development / M.E. Desmond and P.A. Haas -- Isolation of neuroepithelium and formation of minispheres / M.E. Desmond and M.C. Field -- Examination of normal and abnormal placentation in the mouse / M.R. Blackburn -- Palatal dysmorphogenesis. Palate organ culture / B.D. Abbott -- Palatal dysmorphogenesis. Quantitative rt-pcr / G.A. Held and B.D. Abbott -- Transspecies grafting as a tool to understand the basis of murine developmental limb abnormalities / S.M. Bell, C.M. Schreiner and W.J. Scott -- Assessment of laterality defects in rodent embryos / M. Fujinaga -- Cardiac morphogenesis and dysmorphogenesis. I. Normal development / A. Wessels and R. Markwald -- Analysis of two aspects of left-right patterning of the vertebrate heart. Heart tube position and heart tube chirality / A.J. Chin -- Biologically based risk assessment models for developmental toxicity / C. Lau and R.W. Setzer -- Positional cloning / L. Xu and Y. Li -- Gene trapping in embryonic stem cells in vitro to identify novel developmentally regulated genes in the mouse / G.E. Lyons [and others] -- PCR-based cloning of cortically localized rnas from xenopus oocytes / J. Zhang and M.L. King -- Analysis of mRNA expression during preimplantation development / K.E. Latham, E. De la Casa and R.M. Schultz -- Differential screens with subtracted pcr-generated cDNA libraries from subregions of single mouse embryos / A. Shimono and R.R. Behringer -- HPLC-based mRNA differential display / T.B. Knudsen -- Production of transgenic drosophila / M. Fujioka [and others] -- Transgenic manipulation of the sea urchin embryo / J.P. Rast -- Transgenic zebrafish / S. Lin -- Production of avian chimeras and germline transmission / K.L. Woods [and others] -- Incorporation of genetically modified cells in chicken chimeras / L.D. Zajchowski [and others] -- Long-term culture of chicken blastodermal cells (cbcs) and selection of transfected cbcs using antibiotic resistance / Q. Wei, K.L. Woods and R.J. Etches -- Nuclear transplantation and cloning in mammals / K.E. Latham and M.E. Westhusin -- Production of transgenic mice / J. Richa -- Production of transgenic mice with yeast artificial chromosomes / A. Jakobovits, B.T. Lamb and K.R. Peterson -- Gene-targeting strategies / S.S. Cheah and R.R. Behringer -- Chimeric animals and germline transmission / J.S. Khillan -- Conditional gene knockout using cre recombinase / Y. Le and B. Sauer -- Application of cre/loxp in drosophila. Site-specific recombination and transgene coplacement / M.L. Siegal and D.L. Hartl
Dimensions
unknown
Extent
1 online resource (xxv, 530 pages, [8] pages of plates)
File format
unknown
Form of item
online
Isbn
9781592590650
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1385/1592590659
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • (OCoLC)170934659
  • (OCoLC)ocn170934659

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