The Resource Developmental Biology Protocols : Volume III, edited by Rocky S. Tuan, Cecilia W. Lo, (electronic resource)

Developmental Biology Protocols : Volume III, edited by Rocky S. Tuan, Cecilia W. Lo, (electronic resource)

Label
Developmental Biology Protocols : Volume III
Title
Developmental Biology Protocols
Title remainder
Volume III
Statement of responsibility
edited by Rocky S. Tuan, Cecilia W. Lo
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 3rd volume and last volume introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. The 1st and 2nd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis
Member of
Is Subseries of
Dewey number
616.99/465
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • QI6mZ19cMmk
  • D4NsekwB5ts
Language note
English
LC call number
QH491
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Tuan, Rocky S.
  • Lo, Cecilia W.
Series statement
Methods in Molecular Biology,
Series volume
137
http://library.link/vocab/subjectName
  • Developmental biology
  • Developmental Biology
Label
Developmental Biology Protocols : Volume III, edited by Rocky S. Tuan, Cecilia W. Lo, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
Developmental Biology Protocols -- Manipulation Of Developmental Gene Expression and Function -- Ectopic Expression in Drosophila -- Clonal Analysis in the Examination of Gene Function in Drosophila -- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos -- Application of Functional Blocking Antibodies -- Analysis of Gene Expression -- Gene Expression Analyzed by Ribonuclease Protection Assay -- Relative Reverse Transcription-Polymerase Chain Reaction -- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control -- In Situ PCR Detection of HIV Expression in the Human Placenta -- Gene Expression Analysis by In Situ Hybridization -- Radio-Isotopic In Situ Hybridization on Tissue Sections -- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry -- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development -- Multicolor Whole-Mount In Situ Hybridization -- Methods for Double Detection of Gene Expression -- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins -- Monoclonal Antibodies in the Analysis of Embryonic Development -- Models of Morphogenesis and Development -- Mesoderm Induction in Xenopus -- Amphibian Organizer Activity -- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery -- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration -- The Chimeric Human/Mouse Model of Angiogenesis -- Analysis of Embryonic Vascular Morphogenesis -- Epithelial-Mesenchymal Interactions -- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning -- Palate Development -- In Vitro Models and Analysis of Differentiation and Development -- In Vitro Fertilization -- Trophoblast Differentiation -- Bone Marrow-Derived Mesenchymal Progenitor Cells -- Identification, Characterization, and Differentiation of Human Prostate Cells -- Preparation of Chick Striated Muscle Cultures -- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm -- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation -- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells -- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis -- Skeletogenesis -- Studying Early Hematopoiesis Using Avian Blastoderm Cultures -- Isolation and Culture of Mouse Germ Cells -- Cadherin-Mediated Cell-Cell Interactions -- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins -- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells -- Developmental Models Of Diseases -- Pax3 and Vertebrate Development -- Genetic-Engineered Models of Skeletal Diseases I -- Genetic-Engineered Models of Skeletal Diseases II -- Transgenic Mouse Models of Craniofacial Disorders
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (557 p.)
Form of item
online
Isbn
9781280820571
Media category
computer
Media type code
c
Other control number
10.1385/1592590667
Specific material designation
remote
System control number
  • (CKB)1000000000239896
  • (EBL)3036810
  • (SSID)ssj0000293850
  • (PQKBManifestationID)11234273
  • (PQKBTitleCode)TC0000293850
  • (PQKBWorkID)10302667
  • (PQKB)10944746
  • (SSID)ssj0000138028
  • (PQKBManifestationID)12002741
  • (PQKBTitleCode)TC0000138028
  • (PQKBWorkID)10097016
  • (PQKB)11216466
  • (MiAaPQ)EBC3036810
  • (DE-He213)978-1-59259-066-7
  • (EXLCZ)991000000000239896
Label
Developmental Biology Protocols : Volume III, edited by Rocky S. Tuan, Cecilia W. Lo, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
Developmental Biology Protocols -- Manipulation Of Developmental Gene Expression and Function -- Ectopic Expression in Drosophila -- Clonal Analysis in the Examination of Gene Function in Drosophila -- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos -- Application of Functional Blocking Antibodies -- Analysis of Gene Expression -- Gene Expression Analyzed by Ribonuclease Protection Assay -- Relative Reverse Transcription-Polymerase Chain Reaction -- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control -- In Situ PCR Detection of HIV Expression in the Human Placenta -- Gene Expression Analysis by In Situ Hybridization -- Radio-Isotopic In Situ Hybridization on Tissue Sections -- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry -- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development -- Multicolor Whole-Mount In Situ Hybridization -- Methods for Double Detection of Gene Expression -- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins -- Monoclonal Antibodies in the Analysis of Embryonic Development -- Models of Morphogenesis and Development -- Mesoderm Induction in Xenopus -- Amphibian Organizer Activity -- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery -- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration -- The Chimeric Human/Mouse Model of Angiogenesis -- Analysis of Embryonic Vascular Morphogenesis -- Epithelial-Mesenchymal Interactions -- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning -- Palate Development -- In Vitro Models and Analysis of Differentiation and Development -- In Vitro Fertilization -- Trophoblast Differentiation -- Bone Marrow-Derived Mesenchymal Progenitor Cells -- Identification, Characterization, and Differentiation of Human Prostate Cells -- Preparation of Chick Striated Muscle Cultures -- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm -- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation -- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells -- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis -- Skeletogenesis -- Studying Early Hematopoiesis Using Avian Blastoderm Cultures -- Isolation and Culture of Mouse Germ Cells -- Cadherin-Mediated Cell-Cell Interactions -- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins -- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells -- Developmental Models Of Diseases -- Pax3 and Vertebrate Development -- Genetic-Engineered Models of Skeletal Diseases I -- Genetic-Engineered Models of Skeletal Diseases II -- Transgenic Mouse Models of Craniofacial Disorders
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (557 p.)
Form of item
online
Isbn
9781280820571
Media category
computer
Media type code
c
Other control number
10.1385/1592590667
Specific material designation
remote
System control number
  • (CKB)1000000000239896
  • (EBL)3036810
  • (SSID)ssj0000293850
  • (PQKBManifestationID)11234273
  • (PQKBTitleCode)TC0000293850
  • (PQKBWorkID)10302667
  • (PQKB)10944746
  • (SSID)ssj0000138028
  • (PQKBManifestationID)12002741
  • (PQKBTitleCode)TC0000138028
  • (PQKBWorkID)10097016
  • (PQKB)11216466
  • (MiAaPQ)EBC3036810
  • (DE-He213)978-1-59259-066-7
  • (EXLCZ)991000000000239896

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