The Resource Dengue : methods and protocols, edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan

Dengue : methods and protocols, edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan

Label
Dengue : methods and protocols
Title
Dengue
Title remainder
methods and protocols
Statement of responsibility
edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan
Contributor
Editor of compilation
Subject
Genre
Language
eng
Summary
Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease. Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research
Member of
Cataloging source
GW5XE
Dewey number
616.9/1852
Illustrations
illustrations
Index
index present
LC call number
RC137
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
  • Padmanabhan, Radhakrishnan
  • Vasudevan, Subhash
Series statement
Methods in Molecular Biology, Methods and Protocols,
Series volume
1138
http://library.link/vocab/subjectName
  • Dengue
  • Medicine & Public Health
  • Infectious Diseases
  • Virology
  • Laboratory Medicine
Label
Dengue : methods and protocols, edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan
Link
Instantiates
Publication
Note
Includes index
Antecedent source
unknown
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
Dimensions
unknown
Extent
1 online resource (xiii, 403 pages)
File format
unknown
Form of item
online
Isbn
9781493903481
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-0348-1
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4434061-01okla_normanlaw
  • (SIRSI)4434061
  • (Sirsi) o877823584
  • (OCoLC)877823584
Label
Dengue : methods and protocols, edited by Radhakrishnan Padmanabhan, Subhash G. Vasudevan
Link
Publication
Note
Includes index
Antecedent source
unknown
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
Dengue Virus Growth, Purification and Fluorescent Labeling -- Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique -- Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique -- Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy -- Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion -- Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection -- Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging -- Development and Application of Dengue Virus Reverse Genetic Systems -- DENV4 Renilla Luciferase Reporter Replicons -- Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo Recombination -- Identification of Dengue-Specific Human Antibody Fragments Using Phage Display -- Next Generation Whole Genome Sequencing of Dengue Virus -- MPGAfold in Dengue Secondary Structure Prediction -- Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE -- Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 -- Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry -- Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore -- Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation -- Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import -- Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening -- Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral Inhibitors -- Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase -- Animal Models in Dengue -- Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
Dimensions
unknown
Extent
1 online resource (xiii, 403 pages)
File format
unknown
Form of item
online
Isbn
9781493903481
Isbn Type
(electronic bk.)
Level of compression
unknown
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.1007/978-1-4939-0348-1
Other physical details
illustrations (some color).
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4434061-01okla_normanlaw
  • (SIRSI)4434061
  • (Sirsi) o877823584
  • (OCoLC)877823584

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