The Resource Cystic fibrosis methods and protocols, edited by William R. Skach, (electronic resource)

Cystic fibrosis methods and protocols, edited by William R. Skach, (electronic resource)

Label
Cystic fibrosis methods and protocols
Title
Cystic fibrosis methods and protocols
Statement of responsibility
edited by William R. Skach
Contributor
Subject
Genre
Language
eng
Summary
Since the cloning of the cystic fibrosis transmembrane conductance regulator (CFTR) nearly a decade ago, cystic fibrosis researchers, clinicians, and patients have come to rely increasingly on a diverse array of fundamental techniques to understand the molecular basis of this complex disease. Cystic Fibrosis Methods and Protocols consolidates a broad range of detailed and readily reproducible in vitro, cellular, and whole animal laboratory protocols into an indispensable resource. From electrophysiology and cell biology, to animal models and gene therapy, this comprehensive set of methods provides the step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Specific protocols describe new techniques for diagnosis, in vitro methods for the expression and functional analysis of CFTR, novel biochemical and cellular systems to determine how mutations subvert CFTR function, and in vivo protocols to examine how CFTR dysfunction produces multisystem pathology in human and animal models. Comprehensive, multidisciplinary, and highly practical, Cystic Fibrosis Methods and Protocols makes accessible to today's cystic fibrosis investigator the powerful new scientific techniques required to investigate the basic science of the disease and to translate this into effective clinical solutions
Member of
Action
digitized
Cataloging source
COO
Dewey number
616.3/7
Illustrations
illustrations
Index
index present
LC call number
RC858.C95
LC item number
C974 2002
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 25
NLM item number
C997 2002
http://library.link/vocab/relatedWorkOrContributorName
Skach, William
Series statement
Methods in molecular medicine
Series volume
70
http://library.link/vocab/subjectName
  • Cystic fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • Cystic Fibrosis
  • Mucoviscidose
  • MEDICAL
  • Kystische fibrose
  • Wetenschappelijke technieken
  • Cystic fibrosis
Label
Cystic fibrosis methods and protocols, edited by William R. Skach, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591876
Instantiates
Publication
Note
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut
Dimensions
unknown
Extent
1 online resource (xvi, 631 pages)
Form of item
online
Isbn
9780896038974
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)171125821
  • (OCoLC)ocn171125821
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Cystic fibrosis methods and protocols, edited by William R. Skach, (electronic resource)
Link
http://dx.doi.org/10.1385/1592591876
Publication
Note
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
CFTR mutation detection by multiplex heteroduplex (mhet) analysis on mde gel / J. Zielenski [and others]-Cdna microarrays for pharmacogenomic analysis of cystic fibrosis / M. Srivastava, O. Eidelman and H.B. Pollard-Natural animal models of human genetic diseases / J.J. Wine, M. Dean and D. Glavac-Electrophysiological approach to studying CFTR / H. Fischer-Quantitative analysis of atp-dependent gating of CFTR / A. Powe [and others]-CFTR regulation by phosphorylation / T. Zhu [and others]-Transepithelial measurements of bicarbonate secretion in calu-3 cells / R.J. Bridges-Transepithelial impedance analysis of chloride secretion / A.K. Singh [and others]-Studies of the molecular basis for cystic fibrosis using purified reconstituted CFTR protein / I. Kogan [and others]-Probing CFTR channel structure and function using the substituted-cysteine-accessibility method / M.H. Akabas-Methods for the study of intermolecular and intramolecular interactions regulating CFTR function / A.P. Naren-Fluorescent indicator methods to assay functional CFTR expression in cells / A.S. Verkman and S. Jayaraman-Immunolocalization of CFTR in intact tissue and cultured cells / C.R. Marino-Analysis of CFTR trafficking and polarization using green fluorescent protein and confocal microscopy / B.D. Moyer and B.A. Stanton-CFTR folding and maturation in cells / M. Benharouga, M. Sharma and G.L. Lukacs-Isolation of CFTR. Chaperone complexes by co-immunoprecipitation / G.C. Meacham and D.M. Cyr-CFTR expression and er-associated degradation in yeast / Y. Zhang, S. Michaelis and J.L. Brodsky-Manipulating the folding pathway of delta f508 CFTR using chemical chaperones / M. Howard and W.J. Welch-CFTR degradation and aggregation / M.J. Corboy, P.J. Thomas and W.C. Wigley-In vitro reconstitution of CFTR biogenesis and degradation / J. Oberdorf and W.R. Skach-In vitro CFTR folding assays / R.D. Stidham, W.C. Wigley and P.J. Thomas-Analysis of CFTR endocytosis by cell surface biotinylation / K. Weixel and N.A. Bradbury-CFTR regulation of enac / S.H. Donaldson, E.G. Poligone and M.J. Stutts-Yeast two-hybrid identification and analysis of protein interactions with CFTR / V. Raghuram, K.R. Hallows and J.K. Foskett-Biochemical assays for studying indirect interactions between CFTR and the cytoskeleton / P.J. Mohler [and others]-CFTR-associated atp transport and release / M.E. Egan-Inflammatory mediators in CF patients / J.B. Hilliard, M.W. Konstan and P.B. Davis-Bacterial colonization and infection in the CF lung / S.D. Sagel, E.B. Dowell and F.J. Accurso-Antimicrobial peptides and proteins in the CF airway / A.M. Cole and T. Ganz-Bacterial-epithelial interactions / R. Bryan and A. Prince-Thin-film measurements of airway surface liquid volume/composition and mucus transport rates in vitro / R. Tarran and R.C. Boucher-Murine models of CF airway infection and inflammation / J.F. Chmiel, M.W. Konstan and M. Berger-Analysis of lipid abnormalities in CF mice / S.D. Freedman [and others]-Bioelectric measurement of CFTR function in mice / B.R. Grubb-Xenograft model of the CF airway / M. Filali [and others]-Development of conditionally immortalized epithelial cell lines from CF and non-CF mice / C.U. Cotton-Technical approaches to analyze the in vivo ion composition of airway surface liquid / J.M. Zahm [and others]-Design of gene therapy clinical trials in CF patients / K.V. Curlee and E.J. Sorscher-Formulation of synthetic vectors for cystic fibrosis gene therapy / J. Marshall and S.H. Cheng-Adeno-associated viral vectors for CF gene therapy / T.R. Flotte, I. Virella-Lowell and K.A. Chesnut
Dimensions
unknown
Extent
1 online resource (xvi, 631 pages)
Form of item
online
Isbn
9780896038974
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)171125821
  • (OCoLC)ocn171125821
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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