The Resource Corneal regenerative medicine : methods and protocols, edited by Bernice Wright, Che J. Connon, (electronic resource)

Corneal regenerative medicine : methods and protocols, edited by Bernice Wright, Che J. Connon, (electronic resource)

Label
Corneal regenerative medicine : methods and protocols
Title
Corneal regenerative medicine
Title remainder
methods and protocols
Statement of responsibility
edited by Bernice Wright, Che J. Connon
Contributor
Subject
Genre
Language
eng
Summary
Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings
Member of
Cataloging source
GW5XE
Dewey number
617.7/19
Illustrations
illustrations
Index
index present
LC call number
RE336
LC item number
.C67 2013
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
http://library.link/vocab/relatedWorkOrContributorName
  • Wright, Bernice
  • Connon, Che J
Series statement
Methods in molecular biology,
Series volume
1014
http://library.link/vocab/subjectName
  • Cornea
  • Regenerative medicine
Label
Corneal regenerative medicine : methods and protocols, edited by Bernice Wright, Che J. Connon, (electronic resource)
Link
Instantiates
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
  • Enrichment of human corneal epithelial stem/progenitor cells by magnetic bead sorting using SSEA4 as a negative marker
  • Martin N. Nakatsu and Sophie X. Deng
  • Limbal epithelial stem cell identification using immunoblotting analysis
  • Bernice Wright and Che J. Connon
  • Culture of limbal epithelial cells
  • Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder
  • Culture of limbal stromal cells and corneal endothelial cells
  • Naresh Polisetti and Nancy C. Joyce
  • Formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells
  • Shengli Mi and Che J. Connon
  • Limbal epithelial cell therapy : past, present, and future
  • Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry
  • Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett
  • Fabrication of a corneal-limbal tissue substitute using silk fibroin
  • Laura J. Bray ... [et al.]
  • Cultivation of limbal epithelial cells on electrospun poly(lactide-co-glycolide) scaffolds for delivery to the cornea
  • Pallavi Deshpande ... [et al.]
  • Growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds
  • Vladimir Holan, Eliska Javorkova, and Peter Trosan
  • Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels
  • Nicola C. Hunt and Liam M. Grover
  • Tor Paaske Utheim
  • In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans
  • Neil Lagali, May Griffith, and Per Fagerholm
  • Artificial cornea
  • Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith
  • Clonal analysis of limbal epithelial stem cell populations
  • Ursula Schlötzer-Schrehardt
  • Use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation
  • Ashley M. Crane and Sanjoy K. Bhattacharya
Dimensions
unknown
Extent
1 online resource (xii, 230 p.)
File format
unknown
Form of item
online
Isbn
9781627034326
Isbn Type
(electronic bk.)
Level of compression
unknown
Other physical details
ill.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4214344-01okla_normanlaw
  • (SIRSI)4214344
  • (Sirsi) o846581175
  • (OCoLC)846581175
Label
Corneal regenerative medicine : methods and protocols, edited by Bernice Wright, Che J. Connon, (electronic resource)
Link
Publication
Antecedent source
unknown
Bibliography note
Includes bibliographical references and index
Color
multicolored
Contents
  • Enrichment of human corneal epithelial stem/progenitor cells by magnetic bead sorting using SSEA4 as a negative marker
  • Martin N. Nakatsu and Sophie X. Deng
  • Limbal epithelial stem cell identification using immunoblotting analysis
  • Bernice Wright and Che J. Connon
  • Culture of limbal epithelial cells
  • Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder
  • Culture of limbal stromal cells and corneal endothelial cells
  • Naresh Polisetti and Nancy C. Joyce
  • Formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells
  • Shengli Mi and Che J. Connon
  • Limbal epithelial cell therapy : past, present, and future
  • Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry
  • Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett
  • Fabrication of a corneal-limbal tissue substitute using silk fibroin
  • Laura J. Bray ... [et al.]
  • Cultivation of limbal epithelial cells on electrospun poly(lactide-co-glycolide) scaffolds for delivery to the cornea
  • Pallavi Deshpande ... [et al.]
  • Growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds
  • Vladimir Holan, Eliska Javorkova, and Peter Trosan
  • Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels
  • Nicola C. Hunt and Liam M. Grover
  • Tor Paaske Utheim
  • In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans
  • Neil Lagali, May Griffith, and Per Fagerholm
  • Artificial cornea
  • Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith
  • Clonal analysis of limbal epithelial stem cell populations
  • Ursula Schlötzer-Schrehardt
  • Use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation
  • Ashley M. Crane and Sanjoy K. Bhattacharya
Dimensions
unknown
Extent
1 online resource (xii, 230 p.)
File format
unknown
Form of item
online
Isbn
9781627034326
Isbn Type
(electronic bk.)
Level of compression
unknown
Other physical details
ill.
Quality assurance targets
not applicable
Reformatting quality
unknown
Sound
unknown sound
Specific material designation
remote
System control number
  • 4214344-01okla_normanlaw
  • (SIRSI)4214344
  • (Sirsi) o846581175
  • (OCoLC)846581175

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