The Resource Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey

Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey

Label
Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey
Title
Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey
Creator
Contributor
Subject
Language
eng
http://library.link/vocab/creatorName
Vupputuri, Sravanthi
LC call number
TG315 .V97 2013
http://library.link/vocab/relatedWorkOrContributorName
  • Fathepure, Babu
  • Wilber, Gregory
  • Nasrazadani, Seifollah
  • Ley, Tyler
  • Ramsey, Joshua D
http://library.link/vocab/subjectName
  • Bridge structures
  • Acid production
  • Concrete
  • Sulfur oxidizing microorganisms
  • 16S rRNA gene sequence
Label
Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey
Link
http://www.oktc.org/otc/files/finalReports/OTCREOS9.1-39-F.pdf
Instantiates
Publication
Note
  • DTRT06-G-0016
  • Samples obtained from deteriorated bridge structures in Texas were cultured in growth medium containing thiosulfate as an energy source and investigated for acid production, type of acid produced by microbes and the bio-deterioration of concrete cylinders. Enriched cultures decreased the pH to 3.5. Whereas in culture containing concrete the pH remained stable, although there was 97 % oxidation of thiosulfate to sulfate. Bio-deterioration was also evident from the 2-fold higher amount of calcium leached out from concrete compared to controls . Stereo microscope and scanning electron microscopy (SEM) images of the concrete cylinders attacked by acid revealed cracks, which could be due to the formation of expandable products gypsum and ettringite. Microbe responsible for acid production was isolated. The16S rRNA-gene of microbe was genetically sequenced, analyzed with the use of basic local alignment search tool (BLAST) and was identified as Streptomyces sp. This strain was capable of reducing the culture pH to 3.4 in absence of concrete. Whereas in presence of concrete a drop in pH was observed , when there was sufficient amount of thiosulfate for oxidation reaction. There was 98 % oxidation of thiosulfate to sulfate and the amount of calcium leached out from concrete was 6 fold higher than control. SEM images revealed cracks on concrete exposed to Streptomtces sp. culture. Approximately, 400 to 600 16S rRNA-gene sequences representing microbial communities on concrete surfaces from three different bridge structures were analyzed. Highly diverse bacterial and archael communities with only a few known acid producers existed at the time of sampling at all three sites. However, our laboratory studies revealed that the community composition dramatically shifted from a highly diverse to a highly rich dominated by mainly sulfur oxidizing acid-producers such as Thiobacillus thioparus, Alicyclobacillus ferrooxydans, Alicyclobacillus pomorum, Alicyclobacillus acidocaldarius, Alicyclobacillus sp. and Bacillus sp., when thiosulfate was provided. The acid producers were able to oxidize 40 mM thiosulfate to roughly 75 mM sulfate within 5 weeks suggesting almost stoichiometric conversion (94 %) of the added energy source. The pH of the culture decreased from 6.7 to 2.8. These results clearly demonstrate the role of sulfur oxidizing microorganisms in concrete corrosion and the availability of reduced sulfur compounds in the environment is important for corrosion to occur
Extent
78 pages
System control number
(EOS.Web)7591
Label
Characterization and Mediation of Microbial Deterioration of Concrete Bridge Structures / Sravanthi Vupputuri, Babu Fathepure, Gregory Wilber, Seifollah Nasrazadani, Tyler Ley, and Joshua D. Ramsey
Link
http://www.oktc.org/otc/files/finalReports/OTCREOS9.1-39-F.pdf
Publication
Note
  • DTRT06-G-0016
  • Samples obtained from deteriorated bridge structures in Texas were cultured in growth medium containing thiosulfate as an energy source and investigated for acid production, type of acid produced by microbes and the bio-deterioration of concrete cylinders. Enriched cultures decreased the pH to 3.5. Whereas in culture containing concrete the pH remained stable, although there was 97 % oxidation of thiosulfate to sulfate. Bio-deterioration was also evident from the 2-fold higher amount of calcium leached out from concrete compared to controls . Stereo microscope and scanning electron microscopy (SEM) images of the concrete cylinders attacked by acid revealed cracks, which could be due to the formation of expandable products gypsum and ettringite. Microbe responsible for acid production was isolated. The16S rRNA-gene of microbe was genetically sequenced, analyzed with the use of basic local alignment search tool (BLAST) and was identified as Streptomyces sp. This strain was capable of reducing the culture pH to 3.4 in absence of concrete. Whereas in presence of concrete a drop in pH was observed , when there was sufficient amount of thiosulfate for oxidation reaction. There was 98 % oxidation of thiosulfate to sulfate and the amount of calcium leached out from concrete was 6 fold higher than control. SEM images revealed cracks on concrete exposed to Streptomtces sp. culture. Approximately, 400 to 600 16S rRNA-gene sequences representing microbial communities on concrete surfaces from three different bridge structures were analyzed. Highly diverse bacterial and archael communities with only a few known acid producers existed at the time of sampling at all three sites. However, our laboratory studies revealed that the community composition dramatically shifted from a highly diverse to a highly rich dominated by mainly sulfur oxidizing acid-producers such as Thiobacillus thioparus, Alicyclobacillus ferrooxydans, Alicyclobacillus pomorum, Alicyclobacillus acidocaldarius, Alicyclobacillus sp. and Bacillus sp., when thiosulfate was provided. The acid producers were able to oxidize 40 mM thiosulfate to roughly 75 mM sulfate within 5 weeks suggesting almost stoichiometric conversion (94 %) of the added energy source. The pH of the culture decreased from 6.7 to 2.8. These results clearly demonstrate the role of sulfur oxidizing microorganisms in concrete corrosion and the availability of reduced sulfur compounds in the environment is important for corrosion to occur
Extent
78 pages
System control number
(EOS.Web)7591

Library Locations

  • Architecture LibraryBorrow it
    Gould Hall 830 Van Vleet Oval Rm. 105, Norman, OK, 73019, US
    35.205706 -97.445050
  • Bizzell Memorial LibraryBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Boorstin CollectionBorrow it
    401 W. Brooks St., Norman, OK, 73019, US
    35.207487 -97.447906
  • Chinese Literature Translation ArchiveBorrow it
    401 W. Brooks St., RM 414, Norman, OK, 73019, US
    35.207487 -97.447906
  • Engineering LibraryBorrow it
    Felgar Hall 865 Asp Avenue, Rm. 222, Norman, OK, 73019, US
    35.205706 -97.445050
  • Fine Arts LibraryBorrow it
    Catlett Music Center 500 West Boyd Street, Rm. 20, Norman, OK, 73019, US
    35.210371 -97.448244
  • Harry W. Bass Business History CollectionBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • History of Science CollectionsBorrow it
    401 W. Brooks St., Rm. 521NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • John and Mary Nichols Rare Books and Special CollectionsBorrow it
    401 W. Brooks St., Rm. 509NW, Norman, OK, 73019, US
    35.207487 -97.447906
  • Library Service CenterBorrow it
    2601 Technology Place, Norman, OK, 73019, US
    35.185561 -97.398361
  • Price College Digital LibraryBorrow it
    Adams Hall 102 307 West Brooks St., Norman, OK, 73019, US
    35.210371 -97.448244
  • Western History CollectionsBorrow it
    Monnet Hall 630 Parrington Oval, Rm. 300, Norman, OK, 73019, US
    35.209584 -97.445414
Processing Feedback ...