The Resource Cell Engineering : Transient Expression, edited by Mohamed Al-Rubeai, (electronic resource)

Cell Engineering : Transient Expression, edited by Mohamed Al-Rubeai, (electronic resource)

Label
Cell Engineering : Transient Expression
Title
Cell Engineering
Title remainder
Transient Expression
Statement of responsibility
edited by Mohamed Al-Rubeai
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects
Member of
Dewey number
571.31
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
lNgBsGmYH0I
Image bit depth
0
Language note
English
LC call number
QL801-950.9
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Al-Rubeai, Mohamed.
Series statement
Cell Engineering,
Series volume
2
http://library.link/vocab/subjectName
  • Morphology (Animals)
  • Zoology
  • Biotechnology
  • Medical virology
  • Biochemistry
  • Biochemical engineering
  • Animal Anatomy / Morphology / Histology
  • Zoology
  • Biotechnology
  • Virology
  • Biochemistry, general
  • Biochemical Engineering
Label
Cell Engineering : Transient Expression, edited by Mohamed Al-Rubeai, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Bibliography note
Includes bibliographical references at the end of each chapters and index
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Application of Recombinant Baculoviruses in Biopharmaceutical Research -- Engineering Post-Translational Processing of Recombinant Proteins Produced in Insect Cell Culture -- A Guide to Successful Scale-up of the Baculovirus Expression System -- Transient Gene Expression In Mammalian Cells Based on the Calcium Phosphate Transfection Method -- Adenovirus Vectors in Functional Genomics -- Adeno-Associated Virus: A Promising Tool For Gene Delivery -- Alphavirus Vectors: Highly Efficient Systems for Transient Gene Expression -- Transient Expression in Mammalian Cells: Applications and Perspectives
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (VII, 223 p.)
File format
multiple file formats
Form of item
online
Isbn
9789401143158
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-94-011-4315-8
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3400000000123058
  • (SSID)ssj0000922893
  • (PQKBManifestationID)11476237
  • (PQKBTitleCode)TC0000922893
  • (PQKBWorkID)10855538
  • (PQKB)10534000
  • (DE-He213)978-94-011-4315-8
  • (MiAaPQ)EBC3106562
  • (EXLCZ)993400000000123058
Label
Cell Engineering : Transient Expression, edited by Mohamed Al-Rubeai, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Bibliography note
Includes bibliographical references at the end of each chapters and index
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Application of Recombinant Baculoviruses in Biopharmaceutical Research -- Engineering Post-Translational Processing of Recombinant Proteins Produced in Insect Cell Culture -- A Guide to Successful Scale-up of the Baculovirus Expression System -- Transient Gene Expression In Mammalian Cells Based on the Calcium Phosphate Transfection Method -- Adenovirus Vectors in Functional Genomics -- Adeno-Associated Virus: A Promising Tool For Gene Delivery -- Alphavirus Vectors: Highly Efficient Systems for Transient Gene Expression -- Transient Expression in Mammalian Cells: Applications and Perspectives
Dimensions
unknown
Edition
1st ed. 2000.
Extent
1 online resource (VII, 223 p.)
File format
multiple file formats
Form of item
online
Isbn
9789401143158
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-94-011-4315-8
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3400000000123058
  • (SSID)ssj0000922893
  • (PQKBManifestationID)11476237
  • (PQKBTitleCode)TC0000922893
  • (PQKBWorkID)10855538
  • (PQKB)10534000
  • (DE-He213)978-94-011-4315-8
  • (MiAaPQ)EBC3106562
  • (EXLCZ)993400000000123058

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