The Resource Candida Albicans : Methods and Protocols, edited by Ronald L. Cihlar, Richard A. Calderone, (electronic resource)

Candida Albicans : Methods and Protocols, edited by Ronald L. Cihlar, Richard A. Calderone, (electronic resource)

Label
Candida Albicans : Methods and Protocols
Title
Candida Albicans
Title remainder
Methods and Protocols
Statement of responsibility
edited by Ronald L. Cihlar, Richard A. Calderone
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
Over the course of the past decade, there have been remarkable advances in the study of human pathogenic fungi. These developments have taken place throughout a wide range of disciplines, and have come as the result of newly available genome sequences of pathogens such as candida albicans and other model fungi. In Candida Albicans: Methods and Protocols, expert researchers explore these exciting new insights, focusing on the study of medically important fungi and Candida spp in particular. Chapters examine critical aspects of molecular methods, providing information on reporter gene assays, transformation, gene expression in vivo, and methods for large-scale gene disruption. At the same time, the work includes in-depth descriptions of disease models of candidiaisis, facts about strain identification, and guidelines on the preparation of samples for proteomic investigations and tandem affinity purification. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting edge, Candida Albicans: Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology
Member of
Dewey number
614.559 22
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
  • s5gt13pp040
  • acP-xIMNVr4
Image bit depth
0
Language note
English
LC call number
QR1-502
Literary form
non fiction
http://library.link/vocab/relatedWorkOrContributorName
  • Cihlar, Ronald L.
  • Calderone, Richard A.
Series statement
Methods in Molecular Biology,
Series volume
499
http://library.link/vocab/subjectName
  • Microbiology
  • Bacteriology
  • Proteomics
  • Microbiology
  • Bacteriology
  • Proteomics
Label
Candida Albicans : Methods and Protocols, edited by Ronald L. Cihlar, Richard A. Calderone, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Immunogical Methods -- Isolation of Dendritic Cells from Human Blood for In Vitro Interaction Studies with Fungal Antigens -- Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluids -- Phagocytosis and Killing Assays for Candida Species -- Immunization Protocols for Use in Animal Models of Candidiasis -- Virulence and Biofilms -- Penetration of Antifungal Agents Through Candida Biofilms -- Candida Biofilm Analysis in the Artificial Throat Using FISH -- Conditions for Optimal Candida Biofilm Development in Microtiter Plates -- Virulence Measurements: In vitro, ex vivo, and in vivo -- Animal Models of Candidiasis -- Candida albicans Gene Expression in an In Vivo Infection Model -- In Vitro and Ex Vivo Assays of Virulence in Candida albicans -- Strain Typing and Identification -- Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity -- DNA Fingerprinting Candida Species -- Genomics and Proteomics -- The Application of Tandem-Affinity Purification to Candida albicans -- Preparation of Samples for Proteomic Analysis of the Candida albicans Cell Wall -- Reporter Gene Assays in Candida albicans -- Genetic Transformation of Candida albicans -- Large-Scale Gene Disruption Using the UAU1 Cassette -- Standard Growth Media and Common Techniques for Use with Candida albicans
Dimensions
unknown
Edition
1st ed. 2009.
Extent
1 online resource (X, 206 p. 28 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781603271516
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60327-151-6
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000560558
  • (SSID)ssj0000321734
  • (PQKBManifestationID)11246227
  • (PQKBTitleCode)TC0000321734
  • (PQKBWorkID)10280139
  • (PQKB)11229333
  • (DE-He213)978-1-60327-151-6
  • (EXLCZ)991000000000560558
Label
Candida Albicans : Methods and Protocols, edited by Ronald L. Cihlar, Richard A. Calderone, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
Immunogical Methods -- Isolation of Dendritic Cells from Human Blood for In Vitro Interaction Studies with Fungal Antigens -- Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluids -- Phagocytosis and Killing Assays for Candida Species -- Immunization Protocols for Use in Animal Models of Candidiasis -- Virulence and Biofilms -- Penetration of Antifungal Agents Through Candida Biofilms -- Candida Biofilm Analysis in the Artificial Throat Using FISH -- Conditions for Optimal Candida Biofilm Development in Microtiter Plates -- Virulence Measurements: In vitro, ex vivo, and in vivo -- Animal Models of Candidiasis -- Candida albicans Gene Expression in an In Vivo Infection Model -- In Vitro and Ex Vivo Assays of Virulence in Candida albicans -- Strain Typing and Identification -- Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity -- DNA Fingerprinting Candida Species -- Genomics and Proteomics -- The Application of Tandem-Affinity Purification to Candida albicans -- Preparation of Samples for Proteomic Analysis of the Candida albicans Cell Wall -- Reporter Gene Assays in Candida albicans -- Genetic Transformation of Candida albicans -- Large-Scale Gene Disruption Using the UAU1 Cassette -- Standard Growth Media and Common Techniques for Use with Candida albicans
Dimensions
unknown
Edition
1st ed. 2009.
Extent
1 online resource (X, 206 p. 28 illus.)
File format
multiple file formats
Form of item
online
Isbn
9781603271516
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-60327-151-6
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)1000000000560558
  • (SSID)ssj0000321734
  • (PQKBManifestationID)11246227
  • (PQKBTitleCode)TC0000321734
  • (PQKBWorkID)10280139
  • (PQKB)11229333
  • (DE-He213)978-1-60327-151-6
  • (EXLCZ)991000000000560558

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