The Resource Calpain methods and protocols, edited by John S. Elce, (electronic resource)

Calpain methods and protocols, edited by John S. Elce, (electronic resource)

Label
Calpain methods and protocols
Title
Calpain methods and protocols
Statement of responsibility
edited by John S. Elce
Contributor
Subject
Genre
Language
eng
Summary
In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of æ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains
Member of
Action
digitized
Cataloging source
COO
Dewey number
572/.7516
Illustrations
illustrations
Index
index present
LC call number
QP609.C26
LC item number
C34 2000
Literary form
non fiction
NAL call number
QH506
NAL item number
.M45 v. 144
Nature of contents
  • dictionaries
  • bibliography
NLM call number
  • W1 ME9616J v.144 2000
  • QU 136
NLM item number
C1649 2000
http://library.link/vocab/relatedWorkOrContributorName
Elce, John S
Series statement
Methods in molecular biology
Series volume
v. 144
http://library.link/vocab/subjectName
  • Calpain
  • SCIENCE
  • Wetenschappelijke technieken
  • Calpain
  • Calpain
  • Calpain
  • Calpain
Label
Calpain methods and protocols, edited by John S. Elce, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590500
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • 3.
  • Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography
  • Elsa J.B. Murray, Keyvan Behnam and Mario S. Grisanti
  • [and others].
  • 4.
  • Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
  • Dorothy E. Croall.
  • 5.
  • Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain
  • Maurizio Molinari and Ernesto Carafoli.
  • pt. I.
  • 6.
  • Expression of m-Calpain in Escherichia coli
  • John S. Elce
  • Purification of Components of the Calpain System.
  • 1.
  • Purification of [mu]-Calpain, m-Calpain, and Calpastatin from Animal Tissues
  • Valery F. Thompson and Darrel E. Goll.
  • 2.
  • A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpastatin from Small Tissue Samples
  • Jan-Olof Karlsson.
Dimensions
unknown
Extent
1 online resource (xviii, 343 pages)
Form of item
online
Isbn
9780896036321
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)171042338
  • (OCoLC)ocn171042338
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.
Label
Calpain methods and protocols, edited by John S. Elce, (electronic resource)
Link
http://dx.doi.org/10.1385/1592590500
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • 3.
  • Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography
  • Elsa J.B. Murray, Keyvan Behnam and Mario S. Grisanti
  • [and others].
  • 4.
  • Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
  • Dorothy E. Croall.
  • 5.
  • Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain
  • Maurizio Molinari and Ernesto Carafoli.
  • pt. I.
  • 6.
  • Expression of m-Calpain in Escherichia coli
  • John S. Elce
  • Purification of Components of the Calpain System.
  • 1.
  • Purification of [mu]-Calpain, m-Calpain, and Calpastatin from Animal Tissues
  • Valery F. Thompson and Darrel E. Goll.
  • 2.
  • A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpastatin from Small Tissue Samples
  • Jan-Olof Karlsson.
Dimensions
unknown
Extent
1 online resource (xviii, 343 pages)
Form of item
online
Isbn
9780896036321
Isbn Type
(alk. paper)
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Reproduction note
Electronic reproduction.
Specific material designation
remote
System control number
  • (OCoLC)171042338
  • (OCoLC)ocn171042338
System details
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002.

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