Coverart for item
The Resource Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : A Replacement for Western Blot Analysis, by Michael Kinter, Caroline S. Kinter, (electronic resource)

Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : A Replacement for Western Blot Analysis, by Michael Kinter, Caroline S. Kinter, (electronic resource)

Label
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : A Replacement for Western Blot Analysis
Title
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics
Title remainder
A Replacement for Western Blot Analysis
Statement of responsibility
by Michael Kinter, Caroline S. Kinter
Creator
Contributor
Author
Author
Subject
Language
  • eng
  • eng
Summary
A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins
Member of
http://library.link/vocab/creatorName
Kinter, Michael
Dewey number
610
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsaut
  • nrthjwTFPjY
  • 1IZqqCDOrTI
Language note
English
LC call number
R-RZ
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Kinter, Caroline S.
Series statement
  • SpringerBriefs in systems biology
  • SpringerBriefs in Systems Biology,
http://library.link/vocab/subjectName
  • Medicine
  • Proteomics
  • Mass spectrometry
  • Biochemistry
  • Biomedicine general
  • Proteomics
  • Mass Spectrometry
  • Biochemistry, general
Label
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : A Replacement for Western Blot Analysis, by Michael Kinter, Caroline S. Kinter, (electronic resource)
Instantiates
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
The use of mass spectrometry for highly selective detection -- overview of how the selected reaction monitoring experiment works -- Designing a selected reaction monitoring method for a protein -- Example analyses include: sample processing, sample analysis, data processing -- Future Directions
Dimensions
unknown
Edition
1st ed. 2013.
Extent
1 online resource (76 p.)
Form of item
online
Isbn
9781461486664
Media category
computer
Media type code
c
Other control number
10.1007/978-1-4614-8666-4
Specific material designation
remote
System control number
  • (CKB)3710000000019061
  • (EBL)1466681
  • (SSID)ssj0001004885
  • (PQKBManifestationID)11650432
  • (PQKBTitleCode)TC0001004885
  • (PQKBWorkID)11046996
  • (PQKB)10423019
  • (DE-He213)978-1-4614-8666-4
  • (MiAaPQ)EBC1466681
  • (EXLCZ)993710000000019061
Label
Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics : A Replacement for Western Blot Analysis, by Michael Kinter, Caroline S. Kinter, (electronic resource)
Publication
Note
Description based upon print version of record
Bibliography note
Includes bibliographical references
Carrier category
online resource
Carrier category code
cr
Content category
text
Content type code
txt
Contents
The use of mass spectrometry for highly selective detection -- overview of how the selected reaction monitoring experiment works -- Designing a selected reaction monitoring method for a protein -- Example analyses include: sample processing, sample analysis, data processing -- Future Directions
Dimensions
unknown
Edition
1st ed. 2013.
Extent
1 online resource (76 p.)
Form of item
online
Isbn
9781461486664
Media category
computer
Media type code
c
Other control number
10.1007/978-1-4614-8666-4
Specific material designation
remote
System control number
  • (CKB)3710000000019061
  • (EBL)1466681
  • (SSID)ssj0001004885
  • (PQKBManifestationID)11650432
  • (PQKBTitleCode)TC0001004885
  • (PQKBWorkID)11046996
  • (PQKB)10423019
  • (DE-He213)978-1-4614-8666-4
  • (MiAaPQ)EBC1466681
  • (EXLCZ)993710000000019061

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