The Resource Amyloid proteins : methods and protocols, edited by Einar M. Sigurdsson, (electronic resource)

Amyloid proteins : methods and protocols, edited by Einar M. Sigurdsson, (electronic resource)

Label
Amyloid proteins : methods and protocols
Title
Amyloid proteins
Title remainder
methods and protocols
Statement of responsibility
edited by Einar M. Sigurdsson
Contributor
Subject
Genre
Language
eng
Summary
In recent years, various in vitro and in vivo techniques, transgenic technologies, and novel animal models have been developed to understand amyloid diseases-such as Alzheimer's disease and some forms of diabetes-and to screen for drugs that can block amyloid formation and/or disassemble its associated fibrils. In Amyloid Proteins: Methods and Protocols, internationally known researchers describe in step-by-step detail their best techniques for studying these types of proteins and their involvement in the etiology, pathogenesis, diagnosis, and therapy of amyloid diseases. The contributors provide proven methods for the preparation of amyloid and its precursors (oligomers and protofibrils), in vitro assays and analytical techniques for their study, and cell culture models and assays for the production of amyloid proteins. Additional chapters present readily reproducible techniques for amyloid extraction from tissue, its detection in vitro and in vivo, as well as nontransgenic methods for developing amyloid mouse models. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Amyloid Proteins: Methods and Protocols offers both skilled and novice investigators a proven collection of standard and novel techniques for studying amyloid proteins and peptides, as well as their relation to amyloid diseases
Member of
Cataloging source
COO
Dewey number
616.3/995
Illustrations
illustrations
Index
index present
LC call number
QP552.A45
LC item number
A495 2005
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
NLM call number
QU 25
NLM item number
A531 2005
http://library.link/vocab/relatedWorkOrContributorName
Sigurdsson, Einar M
Series statement
Methods in molecular biology
Series volume
v. 299
http://library.link/vocab/subjectName
  • Amyloid
  • Amyloid beta-protein
  • Amyloid
  • Amyloid
  • Biological Assay
  • Cells, Cultured
  • MEDICAL
  • MEDICAL
  • Amyloid
  • Amyloid beta-protein
Label
Amyloid proteins : methods and protocols, edited by Einar M. Sigurdsson, (electronic resource)
Link
http://dx.doi.org/10.1385/1592598749
Instantiates
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • In vitro assays -- Cell culture assays -- In vivo-related assays
  • Preparation of aggregate-free, low molecular weight amyloid-beta for assembly and toxicity assays / G. Bitan and D.B. Teplow -- Determination of Peptide oligomerization state using rapid photochemical crosslinking / S.S. Vollers, D.B. Teplow and G. Bitan -- In vitro preparation of prefibrillar intermediates of amyloid-beta and alpha-synuclein / H.A. Lashuel and D. Grillo-Bosch -- Purification of recombinant tau protein and preparation of Alzheimer-paired helical filaments in vitro / S. Barghorn, J. Biernat and E. Mandelkow -- Cyclic amplification of protein misfolding and aggregation / P. Saa, J. Castilla and C. Soto -- X-ray diffraction studies of amyloid structure / O.S. Makin and L.C. Serpell -- Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis / H.A. Lashuel and J.S. Wall -- Time-lapse atomic force microscopy in the characterization of amyloid-like fibril assembly and oligomeric intermediates / C. Goldsbury and J. Green -- Fourier transform infrared and circular dichroism spectroscopies for amyloid studies / M. Calero and M. Gasset -- Quasielastic light scattering for protein assembly studies / A. Lomakin, D.B. Teplow and G.B. Benedek -- Intrinsic fluorescent detection of tau conformation and aggregation / M. von Bergen, L. Li and E. Mandelkow -- Quantitative measurement of fibrillogenesis by mass spectrometry / A.D. Miranker -- Isolation and culturing of human vascular smooth muscle cells / F.R. Thormodsson and I.H. Olafsson -- Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain / S.S. Jung and E. Levy -- Purification of human wild-type or variant cystatin C from conditioned media of transfected cells / F. Prelli [and others] -- Prion propagation in cell culture / S. Lehmann -- Preparation and propagation of amyloid-enhancing factor / R. Kisilevsky -- Purification of amyloid protein AA subspecies from amyloid-rich human tissues / G.T. Westermark and P. Westermark -- Purification of transthyretin and transthyretin fragments from amyloid-rich human tissues / P. Westermark and G.T. Westermark -- Extraction and chemical characterization of tissue-deposited proteins from minute diagnostic biopsy specimens / F. Goni and G. Gallo -- Tissue processing prior to protein analysis and amyloid-beta quantitation / S.D. Schmidt [and others] -- ELISA method for measurement of amyloid-beta levels / S.D. Schmidt, R.A. Nixon and P.M. Mathews -- Histological staining of amyloid-beta in mouse brains / E.M. Sigurdsson -- The mouse model for scrapie: inoculation, clinical scoring, and histopathological techniques / H.C. Meeker, X. Ye and R.I. Carp -- Radiolabeling of amyloid-beta peptides / M. Calero and J. Ghiso -- In vivo imaging of amyloid-beta deposits in mouse brain with multiphoton microscopy / J. Skoch [and others] -- Magnetic resonance imaging of amyloid plaques in transgenic mice / Y.Z. Wadghiri [and others]
Dimensions
unknown
Extent
1 online resource (xiv, 392 pages)
Form of item
online
Isbn
9781588293374
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)61049338
  • (OCoLC)ocm61049338
Label
Amyloid proteins : methods and protocols, edited by Einar M. Sigurdsson, (electronic resource)
Link
http://dx.doi.org/10.1385/1592598749
Publication
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • In vitro assays -- Cell culture assays -- In vivo-related assays
  • Preparation of aggregate-free, low molecular weight amyloid-beta for assembly and toxicity assays / G. Bitan and D.B. Teplow -- Determination of Peptide oligomerization state using rapid photochemical crosslinking / S.S. Vollers, D.B. Teplow and G. Bitan -- In vitro preparation of prefibrillar intermediates of amyloid-beta and alpha-synuclein / H.A. Lashuel and D. Grillo-Bosch -- Purification of recombinant tau protein and preparation of Alzheimer-paired helical filaments in vitro / S. Barghorn, J. Biernat and E. Mandelkow -- Cyclic amplification of protein misfolding and aggregation / P. Saa, J. Castilla and C. Soto -- X-ray diffraction studies of amyloid structure / O.S. Makin and L.C. Serpell -- Molecular electron microscopy approaches to elucidating the mechanisms of protein fibrillogenesis / H.A. Lashuel and J.S. Wall -- Time-lapse atomic force microscopy in the characterization of amyloid-like fibril assembly and oligomeric intermediates / C. Goldsbury and J. Green -- Fourier transform infrared and circular dichroism spectroscopies for amyloid studies / M. Calero and M. Gasset -- Quasielastic light scattering for protein assembly studies / A. Lomakin, D.B. Teplow and G.B. Benedek -- Intrinsic fluorescent detection of tau conformation and aggregation / M. von Bergen, L. Li and E. Mandelkow -- Quantitative measurement of fibrillogenesis by mass spectrometry / A.D. Miranker -- Isolation and culturing of human vascular smooth muscle cells / F.R. Thormodsson and I.H. Olafsson -- Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy: isolation of smooth muscle and endothelial cells from mouse brain / S.S. Jung and E. Levy -- Purification of human wild-type or variant cystatin C from conditioned media of transfected cells / F. Prelli [and others] -- Prion propagation in cell culture / S. Lehmann -- Preparation and propagation of amyloid-enhancing factor / R. Kisilevsky -- Purification of amyloid protein AA subspecies from amyloid-rich human tissues / G.T. Westermark and P. Westermark -- Purification of transthyretin and transthyretin fragments from amyloid-rich human tissues / P. Westermark and G.T. Westermark -- Extraction and chemical characterization of tissue-deposited proteins from minute diagnostic biopsy specimens / F. Goni and G. Gallo -- Tissue processing prior to protein analysis and amyloid-beta quantitation / S.D. Schmidt [and others] -- ELISA method for measurement of amyloid-beta levels / S.D. Schmidt, R.A. Nixon and P.M. Mathews -- Histological staining of amyloid-beta in mouse brains / E.M. Sigurdsson -- The mouse model for scrapie: inoculation, clinical scoring, and histopathological techniques / H.C. Meeker, X. Ye and R.I. Carp -- Radiolabeling of amyloid-beta peptides / M. Calero and J. Ghiso -- In vivo imaging of amyloid-beta deposits in mouse brain with multiphoton microscopy / J. Skoch [and others] -- Magnetic resonance imaging of amyloid plaques in transgenic mice / Y.Z. Wadghiri [and others]
Dimensions
unknown
Extent
1 online resource (xiv, 392 pages)
Form of item
online
Isbn
9781588293374
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (OCoLC)61049338
  • (OCoLC)ocm61049338

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