The Resource A Beginner’s Guide to Microarrays, edited by Eric M. Blalock, (electronic resource)

A Beginner’s Guide to Microarrays, edited by Eric M. Blalock, (electronic resource)

Label
A Beginner’s Guide to Microarrays
Title
A Beginner’s Guide to Microarrays
Statement of responsibility
edited by Eric M. Blalock
Contributor
Editor
Editor
Subject
Language
  • eng
  • eng
Summary
A Beginner's Guide to Microarrays addresses two audiences - the core facility manager who produces, hybridizes, and scans arrays, and the basic research scientist who will be performing the analysis and interpreting the results. User friendly coverage and detailed protocols are provided for the technical steps and procedures involved in many facets of microarray technology, including: -Cleaning and coating glass slides, -Designing oligonucleotide probes, -Constructing arrays for the detection and quantification of different bacterial species, -Preparing spotting solutions, -Troubleshooting spotting problems, -Setting up and running a core facility, -Normalizing background signal and controlling for systematic variance, -Designing experiments for maximum effect, -Analyzing data with statistical procedures, -Clustering data with machine-learning protocols.
Dewey number
572.8/636
http://bibfra.me/vocab/relation/httpidlocgovvocabularyrelatorsedt
QOt8HUD63wU
Image bit depth
0
Language note
English
LC call number
  • QH345
  • QD415-436
Literary form
non fiction
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Blalock, Eric M.
http://library.link/vocab/subjectName
  • Biochemistry
  • Evolution (Biology)
  • Life sciences
  • Data structures (Computer scienc
  • Trees
  • Biochemistry, general
  • Evolutionary Biology
  • Life Sciences, general
  • Data Structures and Information Theory
  • Tree Biology
Label
A Beginner’s Guide to Microarrays, edited by Eric M. Blalock, (electronic resource)
Instantiates
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
1 Slide Coating And DNA immobilization Chemistries -- Glass Properties -- Glass Cleaning -- Slide Coating Chemistries -- Dna Immobilization Chemistries -- Surface Analysis Methods -- Summary -- References -- 2 Diagnostic Oligonucleotide Micro Arrays For Microbiology -- Scheme of The Experimental Approach -- Sources of Variation -- Establishment of a Sequence Database -- Oligo Length And Melting Temperature (Tm); Designing Oligo Sets Tuned To Work Together -- Oligo Set Design -- Choice Of Oligo/Surface Binding Chemistry -- Array Printing -- Target Preparation -- Hybridisation -- Scanning -- Data Analysis -- Applications In Microbial Identification -- Acknowledgements -- Links Related To Dlagnostic Microbial Microarrays -- Reference List -- 3 Printing Technologies And Microarray Manufacturing Techniques: Making The Perfect Microarray -- Microarray Manufacturing -- Comparing Printing Technologies -- Conclusions -- Acknowledgments -- Reference List -- 4 Arrays For The Masses — Setting Up a Microarray Core Facility -- Preface -- Hedco/Oregon Cancer Institute Spotted Microarray Core at Ohsu -- Core Set-Up -- Array Printing -- The Printer -- Slides -- Amplification -- Printing The Array -- Hybridization -- Scanner -- Other Equipment -- Conclusion -- References -- 5 Microarraydatanormalization: The Art And Science Of Overcoming Technical Variance To Maximize The Detection Of Biologic Variance -- Normalization: Correcting For Technical Variance In Order To Study Biological Variation -- Single Channel Data Normalizations -- Normalizations Of Two-Channel Data -- The Role Of Experimental Design In The Removal Of Technical Variance -- Gene-Specific Normalizations And Clustering -- References -- 6 Experimental Design And Data Analysis -- Measuring RNA -- Fold Change Significance -- Variance -- Experimental Design -- Variance and Fold-Change -- Affymetrix Data -- Working With More Than Two Groups -- Functional Grouping -- Using Excel -- Acknowledgements -- References -- 7 Microarray Experiment Design And Statistic Analysis -- Designing Amicroarray Experiment -- General Procedures For Statistical Analysis Of Microarray Data -- Multiple Hypothesis Testing In Microarray Experiments -- Methods Based On P Value Adjustment -- Analysis Of Variance -- Summary Of The Chapter -- Some Useful Online Sources For Microarray Analysis -- References -- 8 Strategies For Clustering, Classifying Integrating, Standardizing And Visualizing Microarray Gene Expression Data -- Microarray Gene Expression Matrix -- Distance Functions -- Unsupervised Analysis And Clustering Of Microarray Data -- Methods For Validating Unsupervised Analysis -- Supervised Microarray Data Analysis -- Nearest Neighbors -- Support Vector Machines -- Methods To Improve Classifier Performance -- Genetic and Biochemical Networks -- Additional Methods For Microarray Data Analysis -- Microarray Data Visualization -- Microarray Data Standardization And Integration -- Microarray Gene Expression Markup Language (MAGE-ML) -- Microarray Data Repositories -- Challenges In Microarray Gene Expression Data Analysis -- Conclusions -- References
Dimensions
unknown
Edition
1st ed. 2003.
Extent
1 online resource (XVI, 347 p.)
File format
multiple file formats
Form of item
online
Isbn
9781441987600
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-4419-8760-0
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3400000000087752
  • (SSID)ssj0000922448
  • (PQKBManifestationID)11494106
  • (PQKBTitleCode)TC0000922448
  • (PQKBWorkID)10848950
  • (PQKB)11414362
  • (SSID)ssj0000805287
  • (PQKBManifestationID)12352918
  • (PQKBTitleCode)TC0000805287
  • (PQKBWorkID)10823200
  • (PQKB)23954090
  • (DE-He213)978-1-4419-8760-0
  • (MiAaPQ)EBC3073841
  • (EXLCZ)993400000000087752
Label
A Beginner’s Guide to Microarrays, edited by Eric M. Blalock, (electronic resource)
Publication
Note
Bibliographic Level Mode of Issuance: Monograph
Antecedent source
mixed
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
cr
Color
not applicable
Content category
text
Content type code
txt
Contents
1 Slide Coating And DNA immobilization Chemistries -- Glass Properties -- Glass Cleaning -- Slide Coating Chemistries -- Dna Immobilization Chemistries -- Surface Analysis Methods -- Summary -- References -- 2 Diagnostic Oligonucleotide Micro Arrays For Microbiology -- Scheme of The Experimental Approach -- Sources of Variation -- Establishment of a Sequence Database -- Oligo Length And Melting Temperature (Tm); Designing Oligo Sets Tuned To Work Together -- Oligo Set Design -- Choice Of Oligo/Surface Binding Chemistry -- Array Printing -- Target Preparation -- Hybridisation -- Scanning -- Data Analysis -- Applications In Microbial Identification -- Acknowledgements -- Links Related To Dlagnostic Microbial Microarrays -- Reference List -- 3 Printing Technologies And Microarray Manufacturing Techniques: Making The Perfect Microarray -- Microarray Manufacturing -- Comparing Printing Technologies -- Conclusions -- Acknowledgments -- Reference List -- 4 Arrays For The Masses — Setting Up a Microarray Core Facility -- Preface -- Hedco/Oregon Cancer Institute Spotted Microarray Core at Ohsu -- Core Set-Up -- Array Printing -- The Printer -- Slides -- Amplification -- Printing The Array -- Hybridization -- Scanner -- Other Equipment -- Conclusion -- References -- 5 Microarraydatanormalization: The Art And Science Of Overcoming Technical Variance To Maximize The Detection Of Biologic Variance -- Normalization: Correcting For Technical Variance In Order To Study Biological Variation -- Single Channel Data Normalizations -- Normalizations Of Two-Channel Data -- The Role Of Experimental Design In The Removal Of Technical Variance -- Gene-Specific Normalizations And Clustering -- References -- 6 Experimental Design And Data Analysis -- Measuring RNA -- Fold Change Significance -- Variance -- Experimental Design -- Variance and Fold-Change -- Affymetrix Data -- Working With More Than Two Groups -- Functional Grouping -- Using Excel -- Acknowledgements -- References -- 7 Microarray Experiment Design And Statistic Analysis -- Designing Amicroarray Experiment -- General Procedures For Statistical Analysis Of Microarray Data -- Multiple Hypothesis Testing In Microarray Experiments -- Methods Based On P Value Adjustment -- Analysis Of Variance -- Summary Of The Chapter -- Some Useful Online Sources For Microarray Analysis -- References -- 8 Strategies For Clustering, Classifying Integrating, Standardizing And Visualizing Microarray Gene Expression Data -- Microarray Gene Expression Matrix -- Distance Functions -- Unsupervised Analysis And Clustering Of Microarray Data -- Methods For Validating Unsupervised Analysis -- Supervised Microarray Data Analysis -- Nearest Neighbors -- Support Vector Machines -- Methods To Improve Classifier Performance -- Genetic and Biochemical Networks -- Additional Methods For Microarray Data Analysis -- Microarray Data Visualization -- Microarray Data Standardization And Integration -- Microarray Gene Expression Markup Language (MAGE-ML) -- Microarray Data Repositories -- Challenges In Microarray Gene Expression Data Analysis -- Conclusions -- References
Dimensions
unknown
Edition
1st ed. 2003.
Extent
1 online resource (XVI, 347 p.)
File format
multiple file formats
Form of item
online
Isbn
9781441987600
Level of compression
uncompressed
Media category
computer
Media type code
c
Other control number
10.1007/978-1-4419-8760-0
Quality assurance targets
absent
Reformatting quality
access
Specific material designation
remote
System control number
  • (CKB)3400000000087752
  • (SSID)ssj0000922448
  • (PQKBManifestationID)11494106
  • (PQKBTitleCode)TC0000922448
  • (PQKBWorkID)10848950
  • (PQKB)11414362
  • (SSID)ssj0000805287
  • (PQKBManifestationID)12352918
  • (PQKBTitleCode)TC0000805287
  • (PQKBWorkID)10823200
  • (PQKB)23954090
  • (DE-He213)978-1-4419-8760-0
  • (MiAaPQ)EBC3073841
  • (EXLCZ)993400000000087752

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